|
|
| Zap-70 (Ab-319) Antibody, Rabbit Polyclonal Antibody |
 |
|
|
 |
| Catalog Number: 18-785-210480 |
|
|
|
| Related Product Names:
|
-
ZAP70 antibody; ZAP70; SRK; Zap-70 (Ab-319)
- EC 2.7.10.2; 70 kDa zeta-associated protein; Syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70 |
|
- Gene Information -
Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.
|
|
| Gene Name: ZAP70 |
Gene Name Synonym: SRK |
Gi #:
N/A |
NCBI Acc #:
N/A |
Swiss Prot Acc #:
P43403 |
Length (aa): N/A |
Mol. Weight (Da): 69872 |
Chrom Location: N/A |
|
|
 Binding Site: around Tyr319
WB Dilution: 500~1000
IHC Dilution: 50~100
Source/Host: Rabbit
Purity/Purification: Affinity Purified
Clonality: Polyclonal
Crossreactivity: Human, Mouse, Rat
Format: In PBS, 0.02% sodium azide and 50% glycerol.
Storage: 4C for short term (weeks) and -20C for long term. Avoid frequent freeze and thaw.
Stability: 1 year at -20C
Shipping: Products may be shipped on ice pack or dry ice. |
|
| APPLICATIONS for ZAP70 ANTIBODY: |
| IHC, WB: Tested |
|
|
|
|
| ZAP70 ANTIBODY TARGET DESCRIPTION: |
Synonym Names for ZAP70 antibody: ZAP70; SRK; EC 2.7.10.2; 70 kDa zeta-associated protein; Syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70
Function: Associates with the T-cell antigen receptor zeta chain (CD3Z). Plays a role in lymphocyte activation.
Catalytic Activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subunit: Interacts with SLA2 when it is phosphorylated. Interacts with CD3Z and with phosphorylated NFAM1. Interacts with CBLB (By similarity). Interacts with CBL and SLA when it is phosphorylated. The association with SLA (or SLA2) and CBL probably leads to its destruction. Interacts with SHB. Interacts with DEF6 (By similarity).
Tissue Specificity: Expressed in T- and natural killer cells.
Domain: The SH2 domain binds to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z.
Ptm: Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
Disease: Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
Similarity: Contains 1 protein kinase domain.
Similarity: Contains 2 SH2 domains.
Zap-70 (Ab-319) reacts with human, mouse, rat.
OMIM: 176947; gene+phenotype. [NCBI / EBI]
Pathways:
KEGG pathway: Natural killer cell mediated cytotoxicity 04650
KEGG pathway: T cell receptor signaling pathway 04660
Products similar to ZAP70 antibody:
IgG
 Zap-70 (Phospho-Tyr493), IgG
ZAP70 phospho Y315, Y319, IgG
ZAP 70, IgG
Zap-70 (Phospho-Tyr319), IgG
ZAP70 phospho Y292, IgG
Peptide
Zap-70 (Phospho-Tyr493) Antibody Blocking Peptide, Peptide
Zap-70 (Phospho-Tyr319) Antibody Blocking Peptide, Peptide
Tyrosine-protein kinase ZAP-70, Protein
ZAP70 Human, Protein
|
|
| PRODUCT INTERACTIONS: |
Zap-70 (Ab-319) interacts with Proto-oncogene tyrosine-protein kinase LCK, Protein (GenWay Catalog #: 18-785-210480).
Zap-70 (Ab-319) interacts with Lck phospho Y505, IgG (GenWay Catalog #: 18-785-210480).
Zap-70 (Ab-319) interacts with Lck, IgG (GenWay Catalog #: 18-785-210480).
Zap-70 (Ab-319) interacts with Lck [LCK-01], IgG (GenWay Catalog #: 18-785-210480).
Zap-70 (Ab-319) interacts with Lck (Phospho-Tyr393), IgG (GenWay Catalog #: 18-785-210480).
Zap-70 (Ab-319) interacts with Lck (Phospho-Tyr393) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 18-785-210480).
Zap-70 (Ab-319) interacts with LCK, IgG (GenWay Catalog #: 18-785-210480).
Zap-70 (Ab-319) interacts with Lck, IgG (GenWay Catalog #: 18-785-210480).
Zap-70 (Ab-319) interacts with Lck phospho Y192, IgG (GenWay Catalog #: 18-785-210480).
Zap-70 (Ab-319) interacts with Lck phospho S158, IgG (GenWay Catalog #: 18-785-210480).
Zap-70 (Ab-319) interacts with Lck/p56, IgG (GenWay Catalog #: 18-785-210480).
Zap-70 (Ab-319) interacts with LCK Phospho (pY505), IgG (GenWay Catalog #: 18-785-210480).
|
|
|
SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.
|
|
| BACKGROUND
REFERENCES for ZAP70 ANTIBODY: |
| Background references for antibody target are not specific to GenWay products |
[1] Chan A.C., Iwashima M., Turck C.W., Weiss A.
ZAP-70: a 70 kd protein-tyrosine kinase that associates with the TCR zeta chain.
[2] Isakov N., Wange R.L., Burgess W.H., Watts J.D., Aebersold R., Samelson L.E.
ZAP-70 binding specificity to T cell receptor tyrosine-based activation motifs: the tandem SH2 domains of ZAP-70 bind distinct tyrosine-based activation motifs with varying affinity.
[3] Tang J., Sawasdikosol S., Chang J.-H., Burakoff S.J.
SLAP, a dimeric adapter protein, plays a functional role in T cell receptor signaling.
[4] Di Bartolo V., Mege D., Germain V., Pelosi M., Dufour E., Michel F., Magistrelli G., Isacchi A., Acuto O.
Tyrosine 319, a newly identified phosphorylation site of ZAP-70, plays a critical role in T cell antigen receptor signaling.
[5] Lindholm C.K., Henriksson M.L., Hallberg B., Welsh M.
Shb links SLP-76 and Vav with the CD3 complex in Jurkat T cells.
[6] Salomon A.R., Ficarro S.B., Brill L.M., Brinker A., Phung Q.T., Ericson C., Sauer K., Brock A., Horn D.M., Schultz P.G., et al.
Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry.
[7] Brill L.M., Salomon A.R., Ficarro S.B., Mukherji M., Stettler-Gill M., Peters E.C.
Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry.
[8] Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J., et al.
Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.
[9] Tao W.A., Wollscheid B., O'Brien R., Eng J.K., Li X.-J., Bodenmiller B., Watts J.D., Hood L., Aebersold R.
Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometry.
[10] Wissing J., Jaensch L., Nimtz M., Dieterich G., Hornberger R., Keri G., Wehland J., Daub H.
Proteomics analysis of protein kinases by target class-selective prefractionation and tandem mass spectrometry. |
|
Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.
Precautions: ZAP70 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.
Important Notes: During shipment, small volumes of ZAP70 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.
Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about ZAP70 antibody, all related information, articles, resources about ZAP70 antibody are being stored on our online database. Let us know if you have questions regarding this product.
Disclaimer: For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed. |
|
|
|
GenWay is a Protein and Antibody Solutions Provider. Customer Satisfaction is Our Top Priority.
|
|
Thank you for visiting GenWay Biotech's Datasheet. GenWay is the world's leader in chicken, IgY antibody production, producing over 900 quality polyclonal IgY antibodies to date. From our unique recombinant protein production, utilizing a variety of protein expression systems, GenWay's polyclonal IgY antibodies incorporate more epitopes, increasing the success rate for ELISA, western blot, immunohistochemistry (IHC), immunocytochemistry (ICC), and many more immunoassays. |
|
|
|
