Alphabetical Listing of Primary Antibodies:
f
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0-4
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5-9
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A
|
B
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C
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D
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E
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F
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G
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H
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I
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J
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K
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L
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M
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N
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O
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P
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Q
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R
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S
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T
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U
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V
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W
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X
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Y
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Z
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Alphabetical Listing of Proteins:
f
|
0-4
|
5-9
|
A
|
B
|
C
|
D
|
E
|
F
|
G
|
H
|
I
|
J
|
K
|
L
|
M
|
N
|
O
|
P
|
Q
|
R
|
S
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T
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U
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V
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W
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X
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Y
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Z
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Peptides
|
Secondary Antibodies
|
ELISA Kits
Human IFNAR2 - FcA Chimera recombinant protein
Catalog Number: 10-006-220029
Buy IFNAR2 protein -
Size:
0.05 mg ($470.00)
0.025 mg ($260.00)
Related Product Names:
- IFNAR2 protein; IFNAR2; Human IFNAR2 - FcA Chimera
- human cell expressed IFNAR2 - FcA Chimera; IFNAR2; IFNAR2 protein
- Gene Information -
Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact
sales@genwaybio.com
.
Gene Name:
IFNAR2
Gene Name Synonym:
N/A
Gi #:
49456591
NCBI Acc #:
CAG46616.1
Swiss Prot Acc #:
Q6FHD7
Length (aa):
N/A
Mol. Weight (Da):
37393
Chrom Location:
N/A
GenWay Biotech supplies human recombinant proteins expressed from human cells (hcx). This protein is expressed from modified human 293 cells, which has the cellular machinery necessary to attach human post-translational modifications (PTMs) to itself. As such, this protein is different from its recombinant form expressed from other species. GenWay utilizes conventional chromatography purification techniques to purify the proteins. No tag-based affinity chromatography techniques are used for ligands. Each protein has purity greater than 95% by silver stain, with most proteins >97% pure.
Source:
A DNA sequence encoding the signal peptide and extracellular domain of human IFNAR2 (aa 1-243) was fused to the Fc region of human IgG1 (aa 90-330). The chimeric protein was expressed in modified human 293 cells.
Molecular Mass:
Under reducing conditions IFNAR2 - FcB Chimera migrates as a broad band between 60 and 85 kDa on SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with unmodified IFNAR2 - Fc Chimera that has a predicted monomeric molecular mass of 52 kDa.
PI:
IFNAR2 - FcB Chimera separates into a number of glycoforms with a pI between 5.0 and 6.0 on 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified IFNAR2 – Fc Chimera that has a predicted pI of 5.75.
Theoretical Sequence:
ISYDSPDYTD ESCTFKISLR NFRSILSWEL KNHSIVPTHY TLLYTIMSKP EDLKVVKNCA NTTRSFCDLT DEWRSTHEAY VTVLEGFSGN TTLFSCSHNF WLAIDMSFEP PEFEIVGFTN HINVMVKFPS IVEEELQFDL SLVIEEQSEG IVKKHKPEIK GNMSGNFTYI IDKLIPNTNY CVSVYLEHSD EQAVIKSPLK CTLLPPGQES ESAESAKGSS NTKVDKKVEP KSCDKTHTCP PCPAPELLGG PSVFL
Activity:
IFNAR2 - FcB Chimera bound to protein A sepharose beads was able to pull down its ligand, IFNa2b.
Glycosylation:
purified IFNAR2 - FcB Chimera consists of 15-40% carbohydrate by weight.
Reconstitution:
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Purity/Purification:
>95%, as determined by SDS-PAGE and visualized by Coomassie Brilliant Blue.
Crossreactivity:
Human
Format:
When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Storage:
Lyophilized products should be stored at 2 to 8C. Following reconstitution short-term storage at 4C is recommended, with longer-term storage in aliquots at -18 to -20C. Repeated freeze thawing is not recommended.
Shipping:
Products may be shipped on ice pack or dry ice.
TESTING: (
secondary reagents
and
protocols
)
1D GEL:
( anti-IFNAR2 protein )
IFNAR2 protein 1D GEL image
2D GEL:
( anti-IFNAR2 protein )
IFNAR2 protein 2D GEL image
Densitometry:
( anti-IFNAR2 protein )
IFNAR2 protein Densitometry image
IFNAR2 PROTEIN TARGET DESCRIPTION:
Synonym Names for IFNAR2 protein:
IFNAR2; human cell expressed IFNAR2 - FcA Chimera; IFNAR2; IFNAR2 protein
Type I interferons (IFN alpha, beta, omega, tau, kappa and zeta) mediate antiviral, antitumor and immunomodulatory functions and these effects are exerted through binding to the type I interferon receptor (IFNR). This receptor is composed of 2 chains, IFNAR1 and IFNAR2. IFNAR2 is a type I membrane protein tyrosine kinase that is widely expressed in different tissues and is a member of the type II cytokine receptor family. In addition to the membrane bound form a soluble form of the IFNAR2 consisting of the extra-cellular ligand binding domain has been described with the ability to act as a Type 1 IFN agonist, by interacting with and activating cellular membrane-bound IFN receptors, or as an Type I IFN antagonist, with the potential for treatment of autoimmune diseases caused by overproduction of Type 1 IFNs. For a review on Type I Interferons please refer to Oritani et al., (2001) Cytokine Growth Factor Rev. 12(4): 337-48.
N/A
Human IFNAR2 - FcA Chimera reacts with human.
SUGGESTED PROTEIN REAGENTS
- GenWay offers reagents to facilitate your research with cell-based assays, Mass Spectrometry, standards, calibrators, Western blot, cell culture, antibiotics, microwell substrates, and more!
Order Confirmation:
Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.
Precautions:
IFNAR2 protein is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.
Important Notes:
During shipment, small volumes of IFNAR2 protein vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.
Copyright:
This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about IFNAR2 protein, all related information, articles, resources about IFNAR2 protein are being stored on our online database. Let us know if you have questions regarding this product.
Disclaimer:
For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed.
GenWay is a Protein and Antibody Solutions Provider. Customer Satisfaction is Our Top Priority.
GenWay has a diverse line of recombinant protein expression platforms, including E. coli expression, Bacliovirus expression, yeast expression, and mammalian expression. Combining the technologies of bioinformatics, protein domain analysis, gene codon optimization, protein fusion, and protein expression in different host cells, GenWay can produce soluble and functional proteins. In particular, GenWay has the capability and vector systems for expression of difficult proteins such as proteases, kinases, glycosylated and membrane proteins. We have a very high success rate (almost 100%) and can deliver the proteins in a timely fashion. This is a core competency necessary for efficient and effective production of immunogens and affinity ligands for antibody production and purification. GenWay has over 300
recombinant proteins
offered in our catalog.
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