Alphabetical Listing of Primary Antibodies:
f
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0-4
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5-9
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A
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B
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C
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D
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E
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F
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G
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H
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I
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J
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K
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L
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M
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N
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O
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P
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Q
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R
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S
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T
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U
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V
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W
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X
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Y
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Z
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Alphabetical Listing of Proteins:
f
|
0-4
|
5-9
|
A
|
B
|
C
|
D
|
E
|
F
|
G
|
H
|
I
|
J
|
K
|
L
|
M
|
N
|
O
|
P
|
Q
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R
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S
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T
|
U
|
V
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W
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X
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Y
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Z
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Peptides
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Secondary Antibodies
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ELISA Kits
Human IL-5 R alpha (322aa) - FcA Chimera recombinant protein
Catalog Number: 10-006-220051
Buy IL-5 R alpha protein -
Size:
0.02 mg ($260.00)
Related Product Names:
- IL-5 R alpha protein; IL-5 R alpha; Human IL-5 R alpha (322aa) - FcA Chimera
- human cell expressed IL-5 R alpha (322aa) - FcA Chimera; N/A; IL-5 receptor alpha chain; N/A
- Gene Information -
Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact
sales@genwaybio.com
.
Gene Name:
IL-5 R alpha
Gene Name Synonym:
N/A
Gi #:
1247447
NCBI Acc #:
CAA01794.1
Swiss Prot Acc #:
N/A
Length (aa):
420
Mol. Weight (Da):
N/A
Chrom Location:
N/A
GenWay Biotech supplies human recombinant proteins expressed from human cells (hcx). This protein is expressed from modified human 293 cells, which has the cellular machinery necessary to attach human post-translational modifications (PTMs) to itself. As such, this protein is different from its recombinant form expressed from other species. GenWay utilizes conventional chromatography purification techniques to purify the proteins. No tag-based affinity chromatography techniques are used for ligands. Each protein has purity greater than 95% by silver stain, with most proteins >97% pure.
Source:
A DNA sequence encoding the signal peptide and extracellular domain of human IL-5 R alpha (aa 1-342) was fused to the Fc region of human IgG1 (aa 90-330). The chimeric protein was expressed in modified human 293 cells.
Molecular Mass:
Under reducing conditions IL-5 R alpha (322aa) - FcB Chimera migrates as a broad band between 65 and 85 kDa on SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with unmodified IL-5 R alpha (322aa) - Fc Chimera that has a predicted monomeric molecular mass of 64 kDa.
PI:
IL-5 R alpha (322aa) - FcB Chimera separates into a number of glycoforms with a pI between 5.5 and 7.5 on 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified IL-5 R alpha (322aa) – Fc Chimera that has a predicted pI of 6.2.
Theoretical Sequence:
DLLPDEKISL LPPVNFTIKV TGLAQVLLQW KPNPDQEQRN VNLEYQVKIN APKEDDYETR ITESKCVTIL HKGFSASVRT ILQNDHSLLA SSWASAELHA PPGSPGTSIV NLTCTTNTTE DNYSRLRSYQ VSLHCTWLVG TDAPEDTQYF LYYRYGSWTE ECQEYSKDTL GRNIACWFPR TFILSKGRDW LAVLVNGSSK HSAIRPFDQL FALHAIDQIN PPLNVTAEIE GTRLSIQWEK PVSAFPIHCF DYEVK
Activity:
The ED50 of IL-5 R alpha (322aa) - FcB Chimera is typically 0.25 - 0.4 ug/ml as measured by its ability to neutralize IL-5 mediated proliferation of the human growth-factor dependent TF-1 cell line.
% Carbohydrate:
purified IL-5 R alpha (322aa) - FcB Chimera consists of 5-30% carbohydrate by weight.
Glycosylation:
IL-5 R alpha (322aa) - FcB Chimera contains N- and O-linked oligosaccharides.
Reconstitution:
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Purity/Purification:
>95%, as determined by SDS-PAGE and visualized by Coomassie Brilliant Blue.
Crossreactivity:
Human
Format:
When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Storage:
Lyophilized products should be stored at 2 to 8C. Following reconstitution short-term storage at 4C is recommended, with longer-term storage in aliquots at -18 to -20C. Repeated freeze thawing is not recommended.
Shipping:
Products may be shipped on ice pack or dry ice.
TESTING: (
secondary reagents
and
protocols
)
1D GEL:
( anti-IL-5 R alpha protein )
IL-5 R alpha protein 1D GEL image
2D GEL:
( anti-IL-5 R alpha protein )
IL-5 R alpha protein 2D GEL image
Densitometry:
( anti-IL-5 R alpha protein )
IL-5 R alpha protein Densitometry image
IL-5 R ALPHA PROTEIN TARGET DESCRIPTION:
Synonym Names for IL-5 R alpha protein:
IL-5 R alpha; human cell expressed IL-5 R alpha (322aa) - FcA Chimera; N/A; IL-5 receptor alpha chain; N/A
Interleukin 5 (IL-5) plays an important role in B cell development by inducing the terminal differentiation of late-developing B cells. Additionally, IL-5 regulates the differentiation, survival and degranulation of eosinophils. IL-5 is expressed predominately by T-lymphocytes and mast cells, and to a lesser extent by eosinophils, natural killer cells and endothelial cells. IL-5 specifically binds to the IL-5 receptor-alpha (IL-5 R alpha; IL-5Ra) subunit expressed predominately on CD5+ B cells, eosinophils, mast cells, CD34+ stem cells, and basophils. Binding allows association with the common beta subunit (ßc), shared by the IL-3 and GM-CSF receptors. In addition to the membrane bound form of IL-5Ra, soluble forms of IL-5Ra have been identified and are inhibitory to IL-5 activity. There is also evidence to suggest that the levels of membrane bound and soluble IL-5 receptors are altered in disease states such as asthma where there is an increase in the membrane bound form and a concomitant decrease in the soluble receptor form. For a review of the structure and function of IL-5 receptor please see Takatsu K (1995) Yakugaku Zasshi 115:570-583.
N/A
N/A
Human IL-5 R alpha (322aa) - FcA Chimera reacts with human.
SUGGESTED PROTEIN REAGENTS
- GenWay offers reagents to facilitate your research with cell-based assays, Mass Spectrometry, standards, calibrators, Western blot, cell culture, antibiotics, microwell substrates, and more!
BACKGROUND REFERENCES for IL-5 R ALPHA PROTEIN:
Background references for antibody target are not specific to GenWay products
[1]
Devos,R., Fiers,W., Plaetinck,G., Tavernier,J. and van der Heyden,J.
Human Interleukin-5 receptor
Order Confirmation:
Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.
Precautions:
IL-5 R alpha protein is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.
Important Notes:
During shipment, small volumes of IL-5 R alpha protein vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.
Copyright:
This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about IL-5 R alpha protein, all related information, articles, resources about IL-5 R alpha protein are being stored on our online database. Let us know if you have questions regarding this product.
Disclaimer:
For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed.
GenWay is a Protein and Antibody Solutions Provider. Customer Satisfaction is Our Top Priority.
GenWay has a diverse line of recombinant protein expression platforms, including E. coli expression, Bacliovirus expression, yeast expression, and mammalian expression. Combining the technologies of bioinformatics, protein domain analysis, gene codon optimization, protein fusion, and protein expression in different host cells, GenWay can produce soluble and functional proteins. In particular, GenWay has the capability and vector systems for expression of difficult proteins such as proteases, kinases, glycosylated and membrane proteins. We have a very high success rate (almost 100%) and can deliver the proteins in a timely fashion. This is a core competency necessary for efficient and effective production of immunogens and affinity ligands for antibody production and purification. GenWay has over 300
recombinant proteins
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