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| Human TGF-beta 1 recombinant protein |
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| Catalog Number: 10-006-220077 |
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| Related Product Names:
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TGF-beta 1 protein; TGF-beta 1; Human TGF-beta 1
- human cell expressed TGF-beta 1A; TGF-beta activated kinase 1a; Mitogen-activated protein kinase kinase kinase 7 |
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- Gene Information -
Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.
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| Gene Name: TGF-beta 1 |
Gene Name Synonym: N/A |
Gi #:
2924624 |
NCBI Acc #:
BAA25025.1 |
Swiss Prot Acc #:
O43318 |
Length (aa): N/A |
Mol. Weight (Da): 67196 |
Chrom Location: N/A |
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 GenWay Biotech supplies human recombinant proteins expressed from human cells (hcx). This protein is expressed from modified human 293 cells, which has the cellular machinery necessary to attach human post-translational modifications (PTMs) to itself. As such, this protein is different from its recombinant form expressed from other species. GenWay utilizes conventional chromatography purification techniques to purify the proteins. No tag-based affinity chromatography techniques are used for ligands. Each protein has purity greater than 95% by silver stain, with most proteins >97% pure. |
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Source: A DNA sequence encoding the human TGF-beta 1 protein sequence (containing the signal peptide, latency associated peptide and the mature TGF-beta 1 sequence) was expressed in modified human 293 cells. The latency associated peptide is cleaved from the mature protein and removed during purification.
Molecular Mass: TGF-beta 1B migrates at approximately 12-15 kDa on SDS-PAGE. This compares with the predicted molecular mass of 12.8 kDa.
PI: TGF-beta 1B separates into a number of glycoforms with a pI from 8.5 to 10 on 2D PAGE due to post-translational modifications. This compares with the unmodified TGF-beta 1 that has a predicted pI of 8.6.
Theoretical Sequence: ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS
Activity: The ED50 of TGFb-1 is typically 0.1 - 0.5 ng/ml as measured in a cytotoxicity assay using the Mv1Lu mink lung cell line.
Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Purity/Purification: >95%, as determined by SDS-PAGE and visualized by Coomassie Brilliant Blue.
Crossreactivity: Human
Format: When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Storage: Lyophilized products should be stored at 2 to 8C. Following reconstitution short-term storage at 4C is recommended, with longer-term storage in aliquots at -18 to -20C. Repeated freeze thawing is not recommended.
Shipping: Products may be shipped on ice pack or dry ice. |
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| TGF-BETA 1 PROTEIN TARGET DESCRIPTION: |
Synonym Names for TGF-beta 1 protein: TGF-beta 1; human cell expressed TGF-beta 1A; TGF-beta activated kinase 1a; Mitogen-activated protein kinase kinase kinase 7
TGF-beta 1 is a member of the TGF beta superfamily, which includes at least five other isoforms. It is synthesized as a 390 amino acid glycoprotein and contains a 29 amino acid signal sequence and a 249 amino acid latency associated pro-peptide sequence.
TGF-beta 1 is a pleotropic cytokine that regulates various functions, such proliferation and differentiation, of immune and non-immune somatic cells via paracrine and autocrine mechanisms. It is a potent cell growth inhibitor of fibroblasts, hepatocytes, keratinocytes, and endothelial and neuronal cells. For example, TGF-beta 1 can impair the immune response by deactivating macrophages and promoting the regulation of T cells, and it plays a role in T-lymphocyte and natural killer cell growth inhibition, via down-regulating IL-1 and IL-2 mediated proliferative signals. Furthermore, TGF-beta 1 downregulates hematopoietic progenitor cell growth and megakaryocytopoiehsis, and is involved in extracellular matrix (ECM) degradation/ remodeling via inducing ECM protein expression, such as integrins, collagen and fibronectin. It also contributes to bone formation, bone remodeling and participates in regulating chemotaxis and differentiation of osteoblasts.
The abnormal activation or inhibition of TGF-beta regulated processes is implicated in many diseases including cancer, fibrosis and inflammatory disorders such as rheumatoid arthritis and nephritis. TGF-beta 1 also exhibits cardioprotective properties and has been suggested to be an important regulator of contractile function of the heart. For a recent review please refer to Piek, E., Heldin, C.-H., Dijke, P.T. (1999) The FASEB Journal 13:2105-2124.
Function: Component of a protein kinase signal transduction cascade. Mediator of TGF-beta signal transduction. Stimulates NF-kappa-B activation and the p38 MAPK pathway.
Catalytic Activity: ATP + a protein = ADP + a phosphoprotein.
Cofactor: Magnesium.
Subunit: Binds both upstream activators and downstream substrates in multimolecular complexes. Interacts with MAP3K7IP1 and MAP3K7IP2. Interacts with PPM1L.
•GenWay Biotech supplies human recombinant proteins expressed from human cells (hcx). This protein is expressed from modified human 293 cells, which has the cellular machinery necessary to attach human post-translational modifications (PTMs) to itself. As such, this protein is different from its recombinant form expressed from other species. GenWay utilizes conventional chromatography purification techniques to purify the proteins. No tag-based affinity chromatography techniques are used for ligands. Each protein has purity greater than 95% by silver stain, with most proteins >97% pure.
Ptm: Association with MAP3K7IP1 promotes autophosphorylation and subsequent activation.
Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Similarity: Contains 1 protein kinase domain.
Human TGF-beta 1 reacts with human.
OMIM: 602614; gene. [NCBI / EBI]
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SUGGESTED PROTEIN REAGENTS - GenWay offers reagents to facilitate your research with cell-based assays, Mass Spectrometry, standards, calibrators, Western blot, cell culture, antibiotics, microwell substrates, and more!
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Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.
Precautions: TGF-beta 1 protein is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.
Important Notes: During shipment, small volumes of TGF-beta 1 protein vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.
Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about TGF-beta 1 protein, all related information, articles, resources about TGF-beta 1 protein are being stored on our online database. Let us know if you have questions regarding this product.
Disclaimer: For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed. |
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GenWay is a Protein and Antibody Solutions Provider. Customer Satisfaction is Our Top Priority.
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GenWay has a diverse line of recombinant protein expression platforms, including E. coli expression, Bacliovirus expression, yeast expression, and mammalian expression. Combining the technologies of bioinformatics, protein domain analysis, gene codon optimization, protein fusion, and protein expression in different host cells, GenWay can produce soluble and functional proteins. In particular, GenWay has the capability and vector systems for expression of difficult proteins such as proteases, kinases, glycosylated and membrane proteins. We have a very high success rate (almost 100%) and can deliver the proteins in a timely fashion. This is a core competency necessary for efficient and effective production of immunogens and affinity ligands for antibody production and purification. GenWay has over 300 recombinant proteins offered in our catalog. |
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