Alphabetical Listing of Primary Antibodies:
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0-4
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5-9
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A
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B
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C
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D
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E
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F
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G
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H
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I
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J
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K
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L
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M
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N
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O
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P
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Q
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R
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S
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T
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U
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V
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W
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X
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Y
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Z
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Alphabetical Listing of Proteins:
f
|
0-4
|
5-9
|
A
|
B
|
C
|
D
|
E
|
F
|
G
|
H
|
I
|
J
|
K
|
L
|
M
|
N
|
O
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P
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Q
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R
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S
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T
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U
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V
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W
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X
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Y
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Z
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Peptides
|
Secondary Antibodies
|
ELISA Kits
Human TrkB-FcA Chimera recombinant protein
Catalog Number: 10-006-220088
Buy TrkB protein -
Size:
0.025 mg ($260.00)
0.02 mg ($230.00)
Related Product Names:
- TrkB protein; TrkB; Human TrkB-FcA Chimera
- human cell expressed TrkB-FcA Chimera; TrkB [Xenopus]; TrkB
- Gene Information -
Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact
sales@genwaybio.com
.
Gene Name:
TrkB
Gene Name Synonym:
N/A
Gi #:
546785
NCBI Acc #:
AAB30791.1
Swiss Prot Acc #:
Q91373
Length (aa):
N/A
Mol. Weight (Da):
32820
Chrom Location:
N/A
GenWay Biotech supplies human recombinant proteins expressed from human cells (hcx). This protein is expressed from modified human 293 cells, which has the cellular machinery necessary to attach human post-translational modifications (PTMs) to itself. As such, this protein is different from its recombinant form expressed from other species. GenWay utilizes conventional chromatography purification techniques to purify the proteins. No tag-based affinity chromatography techniques are used for ligands. Each protein has purity greater than 95% by silver stain, with most proteins >97% pure.
Source:
A DNA sequence encoding the signal peptide and extracellular domain of human TrkB (aa 1-428) was fused to the Fc region of human IgG1 (aa 93-330). The chimeric protein was expressed in modified human 293 cells.
Molecular Mass:
TrkB-FcB Chimera migrates as a broad band between 90 and 115 kDa on SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified TrkB-Fc Chimera that has a predicted molecular mass of 71.0kDa.
PI:
TrkB-FcB Chimera separates into a number of glycoforms with a pI between 4.9 and 6.7 on 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified TrkB-Fc Chimera that has a predicted pI of 5.79.
Theoretical Sequence:
CPTSCKCSAS RIWCSDPSPG IVAFPRLEPN SVDPENITEI FIANQKRLEI INEDDVEAYV GLRNLTIVDS GLKFVAHKAF LKNSNLQHIN FTRNKLTSLS RKHFRHLDLS ELILVGNPFT CSCDIMWIKT LQEAKSSPDT QDLYCLNESS KNIPLANLQI PNCGLPSANL AAPNLTVEEG KSITLSCSVA GDPVPNMYWD VGNLVSKHMN ETSHTQGSLR ITNISSDDSG KQISCVAENL VGEDQDSVNL TVHFA
Activity:
TrkB-FcB Chimera bound to protein A sepharose beads is able to pull down its ligand, BDNF.
% Carbohydrate:
purified TrkB-FcB Chimera consists of 20-40% carbohydrate by weight.
Glycosylation:
TrkB-FcB Chimera has N-linked and may have O-linked oligosaccharides.
Reconstitution:
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Purity/Purification:
>95%, as determined by SDS-PAGE and visualized by Coomassie Brilliant Blue.
Crossreactivity:
Human
Format:
When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Storage:
Lyophilized products should be stored at 2 to 8C. Following reconstitution short-term storage at 4C is recommended, with longer-term storage in aliquots at -18 to -20C. Repeated freeze thawing is not recommended.
Shipping:
Products may be shipped on ice pack or dry ice.
TESTING: (
secondary reagents
and
protocols
)
1D GEL:
( anti-TrkB protein )
TrkB protein 1D GEL image
2D GEL:
( anti-TrkB protein )
TrkB protein 2D GEL image
Densitometry:
( anti-TrkB protein )
TrkB protein Densitometry image
TRKB PROTEIN TARGET DESCRIPTION:
Synonym Names for TrkB protein:
TrkB; human cell expressed TrkB-FcA Chimera; TrkB [Xenopus]; TrkB
The tropomyosin-related kinase (Trk) family consists of TrkA, TrkB and TrkC. These receptors each bind to and are activated by one or more of the neurotrophins, which include neural growth factor (NGF), brain derived neurotrophic factor (BDNF) and neurotrophic factors 3 and 4/5 (NT-3, NT-4/5). TrkB is a high affinity receptor for BDNF and NT-4/5, and also binds NT-3 with lower affinity. TrkB is widely expressed, primarily in nervous tissue. The biological effects of TrkB activation are crucial in neuronal differentiation during embryonic development, specifically in the formation of the sympathetic nervous system. Additionally, the TrkB/BDNF complex facilitates the survival of post-mitotic neurons, axon growth and guidance as well as synaptic plasticity. TrkB also contributes to learning and spatial memory formation and the attenuation of injury-induced neuronal cell death. In addition to regulating CNS physiology TrkB and BDNF are involved in the formation of follicular growth and oocyte survival in the mammalian ovary.TrkB is a heavily glycosylated type I transmembrane protein of 791 amino acids, and the TrkB-Fc Chimera contains 13 potential N-linked glycosylation sites. For a review of the role of TrkB in neuronal signal transduction please refer to Huang EJ & Reichardt LF (2003) Annu Rev Biochem. 72: 609-42.
Similarity:
Belongs to the protein kinase superfamily. Tyr protein kinase family.
Human TrkB-FcA Chimera reacts with human.
Products similar to TrkB protein:
IgG
TrkB [1.A.37], IgG
TrkB, IgG
SUGGESTED PROTEIN REAGENTS
- GenWay offers reagents to facilitate your research with cell-based assays, Mass Spectrometry, standards, calibrators, Western blot, cell culture, antibiotics, microwell substrates, and more!
Order Confirmation:
Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.
Precautions:
TrkB protein is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.
Important Notes:
During shipment, small volumes of TrkB protein vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.
Copyright:
This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about TrkB protein, all related information, articles, resources about TrkB protein are being stored on our online database. Let us know if you have questions regarding this product.
Disclaimer:
For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed.
GenWay is a Protein and Antibody Solutions Provider. Customer Satisfaction is Our Top Priority.
GenWay has a diverse line of recombinant protein expression platforms, including E. coli expression, Bacliovirus expression, yeast expression, and mammalian expression. Combining the technologies of bioinformatics, protein domain analysis, gene codon optimization, protein fusion, and protein expression in different host cells, GenWay can produce soluble and functional proteins. In particular, GenWay has the capability and vector systems for expression of difficult proteins such as proteases, kinases, glycosylated and membrane proteins. We have a very high success rate (almost 100%) and can deliver the proteins in a timely fashion. This is a core competency necessary for efficient and effective production of immunogens and affinity ligands for antibody production and purification. GenWay has over 300
recombinant proteins
offered in our catalog.
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