DDB1 antibody, Rabbit anti DDB1 (a.a. 198-213) Antibody, Rabbit Polyclonal Antibody

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Peptides \| Secondary Antibodies \| ELISA Kits

Rabbit anti DDB1 (a.a. 198-213) Antibody, Rabbit Polyclonal Antibody

Catalog Number: 18-511-244065

Buy DDB1 antibody -

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Related Product Names:

- DDB1 antibody; DDB1; XAP1; Rabbit anti DDB1 (a.a. 198-213)
- Rabbit anti DDB1 (a.a. 198-213); Damage-specific DNA-binding protein 1; UV-damaged DNA-binding factor; DDB p127 subunit; DNA damage-binding protein a; DDBa; UV-damaged DNA-binding protein 1; UV-DDB 1; Xeroderma pigmentosum group E-complementing protein; X; DNA damage-binding protein 1

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: DDB1	Gene Name Synonym: XAP1
Gi #: N/A	NCBI Acc #: NP_001914.3	Swiss Prot Acc #: Q16531
Length (aa): N/A	Mol. Weight (Da): 126968	Chrom Location: N/A

Host Animal: Rabbit

Immunogen: A synthetic peptide corresponding to amino acids 198-213 of Human DDB1 (internal) coupled to KLH. Sequence: REKEFNKGPWKQENVE

Specificity: Damage-Specific DNA Binding Protein 1, DDB1 (a.a. 198-213)

Specificity: Cross reacts with human and mouse DDB1. Cross reactivity with DDB1 from other sources is not known.

Type of Product: Polyclonal Antibodies for Cancer Research

Concentration: Total Protein: Lot specific (Refractometry)

Preservatives: NaN3

Buffer: Not applicable.

Applications Notes : Suitable for use in ELISA (1:2,000 – 1:10,000) and Western blot (1:500 – 1:1,000). The antibody immunoprecipitates in vitro translated protein and protein from cell lysates (using HeLa, NIH-3T3, and others). A 127 kDa band corresponding to human DDB1 is detected. Coimmunoprecipitation of related proteins has not been tested. Most cell lines expressing DDB1 can be used as a positive control. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Warning: This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1â€“1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.

Source/Host: Rabbit

Purity/Purification: Delipidation and defibrination Product is sterile filtered.

Clonality: Polyclonal

Format: Neat, Liquid

Storage: Upon receipt, aliquot and store at -20 C. Avoid multiple freeze/thaw cycles. Prepare working dilution only prior to immediate use.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for DDB1 ANTIBODY:

ELISA, IP , WB: Tested

TESTING: (secondary reagents and protocols )

Not Available

DDB1 ANTIBODY TARGET DESCRIPTION:

Synonym Names for DDB1 antibody: DDB1; XAP1; Rabbit anti DDB1 (a.a. 198-213); Damage-specific DNA-binding protein 1; UV-damaged DNA-binding factor; DDB p127 subunit; DNA damage-binding protein a; DDBa; UV-damaged DNA-binding protein 1; UV-DDB 1; Xeroderma pigmentosum group E-complementing protein; X; DNA damage-binding protein 1

Rabbit anti DDB1 (a.a. 198-213). Rabbit Antibody to Human Damage-Specific DNA Binding Protein 1 (DDB1, DDB p127 subunit), (amino acids 198-213)

Function: Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2.

Pathway: Protein modification; protein ubiquitination.

Subunit: Component of the UV-DDB complex which includes DDB1 and DDB2. The UV-DDB complex interacts with monoubiquitinated histone H2A and binds to XPC via the DDB2 subunit. Component of numerous DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which consist of a core of DDB1, CUL4A or CUL4B and RBX1. DDB1 may recruit specific substrate targeting subunits to the DCX complex. These substrate targeting subunits are generally known as DCAF (DDB1- and CUL4-associated factor) or CDW (CUL4-DDB1-associated WD40-repeat) proteins. Interacts with AMBRA1, ATG16L1, BTRC, C2ORF37, C4ORF30, DCAF15, DDA1, DET1, DTL, ERCC8, FBXW5, FBXW8, GRWD1, IQWD1, KATNB1, NLE1, NUP43, PAFAH1B1, PHIP, PWP1, RBBP4, RBBP5, RBBP7, RFWD2, VPRBP, WDR5, WDR5B, WDR12, WDR21A, WDR22, WDR23, WDR26, WDR32, WDR39, WDR40A, WDR42, WDR42A, WDR53, WDR57, WDR59, WDR61, WDR68, WSB1, WSB2 and WDTC1. DCX complexes may associate with the COP9 signalosome, and this inhibits the E3 ubiquitin-protein ligase activity of the complex. Interacts with NF2, TSC1 and TSC2. Interacts with Simian virus 5 protein V and the HBV X protein. Interaction with SV5 protein V may prevent the recruitment of DCAF proteins to DCX complexes.

Subcellular Location: Cytoplasm. Nucleus. Note=Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage.

Ptm: Ubiquitinated by CUL4A. Subsequently degraded by ubiquitin-dependent proteolysis.

Similarity: Belongs to the DDB1 family.

OMIM: 600045; gene. [NCBI / EBI]

Pathways:
Reactome Event:DNA Repair 73894

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PRODUCT INTERACTIONS:

Rabbit anti DDB1 (a.a. 198-213) interacts with damage-specific DNA binding protein 2, 48kDa (DDB2), mRNA, IgG (GenWay Catalog #: 18-511-244065).

Rabbit anti DDB1 (a.a. 198-213) interacts with Anti-Human p48-DDB2 (C-terminal specific), IgG (GenWay Catalog #: 18-511-244065).

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

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Precautions: DDB1 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of DDB1 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about DDB1 antibody, all related information, articles, resources about DDB1 antibody are being stored on our online database. Let us know if you have questions regarding this product.

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