PAPPA native protein, PAPP-A native protein

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PAPP-A native protein

Catalog Number: 11-511-248609

Buy PAPPA native protein -

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Related Product Names:

- PAPPA native protein; PAPPA; PAPP-A
- PAPP-A; EC 3.4.24.79; Pregnancy-associated plasma protein A; PAPP-A; Insulin-like growth factor-dependent IGF-binding protein 4 protease; IGF-dependent IGFBP-4 protease; IGFBP-4ase; Pappalysin-1

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: PAPPA	Gene Name Synonym: N/A
Gi #: N/A	NCBI Acc #: NP_002572.2	Swiss Prot Acc #: Q13219
Length (aa): N/A	Mol. Weight (Da): 181141	Chrom Location: N/A

Specificity: Pregnancy Associated Plasma Protein A (PAPP-A)

Type of Product: Purified Hormones and Steroids

Inactivation: Not applicable.

Buffer: Lyophilized from 10mM Tris-HCl, 0.15M Sodium chloride, pH 7.5

Applications Notes : Tested in immunofluorescent assay with monoclonal antibodies specific to PAPP-A. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Concentration: Concentration is lot specific

Source/Host: Retroplacental Blood

Purity/Purification: >85% pure (SDS-PAGE). Ion exchange chromatography, affinity chromatography, gel-filtration and negative immunoaffinity chromatography

Format: Purified, Lyophilized Reconstitute with sterile distilled water.

Storage: Upon receipt, store at -20 C.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for PAPPA NATIVE PROTEIN:

ELISA: Tested

TESTING: (secondary reagents and protocols )

Not Available

PAPPA NATIVE PROTEIN TARGET DESCRIPTION:

Synonym Names for PAPPA native protein: PAPPA; PAPP-A; EC 3.4.24.79; Pregnancy-associated plasma protein A; PAPP-A; Insulin-like growth factor-dependent IGF-binding protein 4 protease; IGF-dependent IGFBP-4 protease; IGFBP-4ase; Pappalysin-1

PAPP-A. Pregnancy Associated Plasma Protein A (PAPP-A). Heterotetrameric complex, consisting of PAPP-A and proMBP subunits

Function: Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF.

Catalytic Activity: Cleavage of the 135-Met-|-Lys-136 bond in insulin-like growth factor binding protein (IGFBP)-4, and the 143-Ser-|-Lys-144 bond in IGFBP-5.

Cofactor: Binds 1 zinc ion per subunit (By similarity).

Enzyme Regulation: Inhibited by complexation with the proform of PRG2.

Subunit: Homodimer; disulfide-linked. In pregnancy serum, predominantly found as a disulfide-linked 2:2 heterotetramer with the proform of PRG2.

Subcellular Location: Secreted.

Tissue Specificity: High levels in placenta and pregnancy serum. In placenta, expressed in X cells in septa and anchoring villi, and in syncytiotrophoblasts in the chorionic villi. Lower levels are found in a variety of other tissues including kidney, myometrium, endometrium, ovaries, breast, prostate, bone marrow, colon, fibroblasts and osteoblasts.

Developmental Stage: Present in serum and placenta during pregnancy; levels increase throughout pregnancy.

Induction: By 8-bromoadenosine-3',5'-phosphate.

Ptm: There appear to be no free sulfhydryl groups.

Similarity: Belongs to the peptidase M43B family [view classification].

Similarity: Contains 5 Sushi (CCP/SCR) domains.

OMIM: 176385; gene. [NCBI / EBI]

Products similar to PAPPA native protein:

IgG

PAPP A [11E4], IgG

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Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: PAPPA native protein is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of PAPPA native protein vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about PAPPA native protein, all related information, articles, resources about PAPPA native protein are being stored on our online database. Let us know if you have questions regarding this product.

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