Vcam1 antibody, Rat Anti-Mouse CD106/VCAM-1 Antibody, Rat Monoclonal Antibody

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Peptides \| Secondary Antibodies \| ELISA Kits

Rat Anti-Mouse CD106/VCAM-1 Antibody, Rat Monoclonal Antibody

Catalog Number: 20-787-275170

Buy Vcam1 antibody -

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Related Product Names:

- Vcam1 antibody; Vcam1; Vcam-1; Rat Anti-Mouse CD106/VCAM-1
- Rat Anti-Mouse CD106/VCAM-1-FITC; V-CAM 1; CD106 antigen; VCAM-1 [Mus musculus]; Vascular cell adhesion protein 1

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: Vcam1	Gene Name Synonym: Vcam-1
Gi #: 298117	NCBI Acc #: CAA47989.1	Swiss Prot Acc #: P29533
Length (aa): 739	Mol. Weight (Da): 81317	Chrom Location: N/A

Isotype: Rat IgG1 kappa

Clone: M/K-2

Characterization: To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested for conformance with characteristics of a standard reference reagent using immunofluorescence staining and analysis by flow cytometry on a Becton Dickinson FACScan™.

Specificity: CD106/VCAM-1 (~Mr 100-kDa), VCAM-1 is an adhesion molecule and a major mediator of the inflammatory response. It is expressed on activated microvascular endothelial cells in response to signals arising from immune responses in infection, graft rejection, tumor recognition and killing. The complementary binding ligand for VCAM-1 is VLA-4/CD49d. In addition to VCAM-1, VLA-4 also recognizes the extracellular matrix molecule fibronectin. This pairing of VCAM-1 and VLA-4 is able to provide a second signal (i.e., non-antigen specific) for T cell stimulation, such as that seen in transplantation. The monoclonal antibody MK-2 has been used in transplant studies to suppress cardiac rejection and induce long-term cardiac graft survival. In addition to inflammatory responses, VCAM-1 has a significant role in hemopoiesis through its ability to retain lymphocyte and myeloid precursors on stromal cells in the marrow and lymphoid organs. CD106/VCAM-1 exists as an integral membrane protein. The M/K-2 monoclonal antibody immunoprecipitates a peptide that gives a single band on SDS-PAGE gels with an apparent Mr of ~100 kDa under reducing conditions and 92 kDa under nonreducing conditions. 1-5

Immunofluorescent Staining: Product: Rat Anti-Mouse CD106-FITC, Amount Used: 1 ug/106 cells, BALB/c bone marrow cells were stained with rat anti-mouse CD106/VCAM-1-FITC, following which large cells were gated and analyzed by flow cytometry.

Working Dilution: Flow Cytometry: 1 ug/106 cells

Warning: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Source/Host: Rat

Clonality: Monoclonal

Crossreactivity: Mouse

Format: Fluorescein (FITC) Conjugate

Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C.
The fluorescein (FITC) conjugate is supplied as 0.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for VCAM1 ANTIBODY:

Flow Cytometry (FACS), IF, IHC: Tested

TESTING: (secondary reagents and protocols )

Flow Cytometry: ( anti-Vcam1 )

Vcam1 antibody Flow Cytometry image
Vcam1

VCAM1 ANTIBODY TARGET DESCRIPTION:

Synonym Names for Vcam1 antibody: Vcam1; Vcam-1; Rat Anti-Mouse CD106/VCAM-1-FITC; V-CAM 1; CD106 antigen; VCAM-1 [Mus musculus]; Vascular cell adhesion protein 1

Function: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.

Subcellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein.

Subcellular Location: Isoform 2: Cell membrane; Lipid-anchor, GPI-anchor.

Tissue Specificity: Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue. Expressed in the bone marrow.

Ptm: The GPI-anchor is located on position 319 of isoform 2.

Similarity: Contains 7 Ig-like C2-type (immunoglobulin-like) domains.

Rat Anti-Mouse CD106/VCAM-1 reacts with mouse.

Products similar to Vcam1 antibody:

IgG

Rat Anti-Mouse CD106/VCAM-1, IgG

VASCULAR CELL ADHESION MOLECULE 1 (VCAM-1), IgG

VCAM1 [1G11B1] (Biotin), IgG

VCAM-1. CD106, IgG

Protein

VCAM-1: Human, Recombinant, E Coli, Protein

VCAM-1 Fragment: Human, Recombinant, E Coli, Protein

VCAM-1: Human, Recombinant, E Coli, Protein

VCAM-1 Fragment: Human, Recombinant, E Coli, Protein

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

BACKGROUND REFERENCES for VCAM1 ANTIBODY:

Background references for antibody target are not specific to GenWay products

1. Miyake, K., I.L. Weissman, J.S. Greenberger and P. W. Kincade. 1991. J. Exp. Med. 173:599.
2. Miyake, K., et al.. 1991. J. Cell. Biol. 114(3):557. .
3. Schlegel, P.G., et al. 1995. J. Immunol. 155:3856. .
4. Bergese, S., et al. 1995. Am J. Phathol. 146:989. .
5. Orosz, C.G., et al. 1993. Transplantation 56:453.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: Vcam1 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of Vcam1 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about Vcam1 antibody, all related information, articles, resources about Vcam1 antibody are being stored on our online database. Let us know if you have questions regarding this product.

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