PAPPA protein, PAPP-A Fragment: Human, Recombinant, E Coli recombinant protein

Alphabetical Listing of Primary Antibodies:	f\| 0-4 \| 5-9 \| A \| B \| C \| D \| E \| F \| G \| H \| I \| J \| K \| L \| M \| N \| O \| P \| Q \| R \| S \| T \| U \| V \| W \| X \| Y \| Z \|
Alphabetical Listing of Proteins:	f\| 0-4 \| 5-9 \| A \| B \| C \| D \| E \| F \| G \| H \| I \| J \| K \| L \| M \| N \| O \| P \| Q \| R \| S \| T \| U \| V \| W \| X \| Y \| Z \|
Peptides \| Secondary Antibodies \| ELISA Kits

PAPP-A Fragment: Human, Recombinant, E Coli recombinant protein

Catalog Number: 10-731-288126

Buy PAPPA protein -

Size:

Related Product Names:

- PAPPA protein; PAPPA; PAPP-A Fragment: Human, Recombinant, E Coli
- Pappalysin-1; Pregnancy-Associated Plasma Protein-1;Insulin-like growth factor-dependent IGF binding protein-4, EC3.4.24.79; IGF-dependent IGFBP-4 protease; IGFBP-4ase.; EC 3.4.24.79; Pregnancy-associated plasma protein A; PAPP-A; Insulin-like growth fact; Pappalysin-1

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: PAPPA	Gene Name Synonym: N/A
Gi #: N/A	NCBI Acc #: NP_002572.2	Swiss Prot Acc #: Q13219
Length (aa): N/A	Mol. Weight (Da): 181141	Chrom Location: N/A

Source/Host: E Coli

Purity/Purification: >95% by SDS-PAGE

Format: Liquid

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for PAPPA PROTEIN:

SDS-Page: Tested

TESTING: (secondary reagents and protocols )

Test : ( anti-PAPPA protein )

PAPPA protein Test image
PAPPA

PAPPA PROTEIN TARGET DESCRIPTION:

Synonym Names for PAPPA protein: PAPPA; Pappalysin-1; Pregnancy-Associated Plasma Protein-1;Insulin-like growth factor-dependent IGF binding protein-4, EC3.4.24.79; IGF-dependent IGFBP-4 protease; IGFBP-4ase.; EC 3.4.24.79; Pregnancy-associated plasma protein A; PAPP-A; Insulin-like growth fact; Pappalysin-1

PAPP-A is a large zinc binding protein, which acts as ametalloprotease and has been shown to cleave insulin-like growth factor binding protein-4. PAPP-A can act as a regulator of IGFbioactivity in several biological systems, including the human ovaryand cardiovascular systems. Recent studies have shown that levels are elevated in patients with unstable angina or acute myocardialinfarction. PAPP-A is also produced in high concentrations duringpregnancy and is released into the maternal circulation. Low levelsof PAPP-A have been linked with a number of foetal chromosomalabnormalities, as well as pre-eclampsia and stillbirth.
RANDOX recombinant PAPP-A comprises a 191 amino acidfragment (81-271) corresponding to the PAPP-A ‘Jelly-Roll’ domain fragment and is expressed in E. coli with an amino-terminal hexahistidine tag. This product is for research use only and is not intended for diagnostic or therapeutic use.

Function: Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF.

Catalytic Activity: Cleavage of the 135-Met-|-Lys-136 bond in insulin-like growth factor binding protein (IGFBP)-4, and the 143-Ser-|-Lys-144 bond in IGFBP-5.

Cofactor: Binds 1 zinc ion per subunit (By similarity).

Enzyme Regulation: Inhibited by complexation with the proform of PRG2.

Subunit: Homodimer; disulfide-linked. In pregnancy serum, predominantly found as a disulfide-linked 2:2 heterotetramer with the proform of PRG2.

Subcellular Location: Secreted.

Tissue Specificity: High levels in placenta and pregnancy serum. In placenta, expressed in X cells in septa and anchoring villi, and in syncytiotrophoblasts in the chorionic villi. Lower levels are found in a variety of other tissues including kidney, myometrium, endometrium, ovaries, breast, prostate, bone marrow, colon, fibroblasts and osteoblasts.

Developmental Stage: Present in serum and placenta during pregnancy; levels increase throughout pregnancy.

Induction: By 8-bromoadenosine-3',5'-phosphate.

Ptm: There appear to be no free sulfhydryl groups.

Similarity: Belongs to the peptidase M43B family [view classification].

Similarity: Contains 5 Sushi (CCP/SCR) domains.

OMIM: 176385; gene. [NCBI / EBI]

Products similar to PAPPA protein:

IgG

PAPP A [11E4], IgG

SUGGESTED PROTEIN REAGENTS - GenWay offers reagents to facilitate your research with cell-based assays, Mass Spectrometry, standards, calibrators, Western blot, cell culture, antibiotics, microwell substrates, and more!

BACKGROUND REFERENCES for PAPPA PROTEIN:

Background references for antibody target are not specific to GenWay products

1: Conover, C.A. et al., 2004 Development 131 (5) 1187-1194

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: PAPPA protein is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of PAPPA protein vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about PAPPA protein, all related information, articles, resources about PAPPA protein are being stored on our online database. Let us know if you have questions regarding this product.

Disclaimer: For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed.

GenWay is a Protein and Antibody Solutions Provider. Customer Satisfaction is Our Top Priority.

GenWay has a diverse line of recombinant protein expression platforms, including E. coli expression, Bacliovirus expression, yeast expression, and mammalian expression. Combining the technologies of bioinformatics, protein domain analysis, gene codon optimization, protein fusion, and protein expression in different host cells, GenWay can produce soluble and functional proteins. In particular, GenWay has the capability and vector systems for expression of difficult proteins such as proteases, kinases, glycosylated and membrane proteins. We have a very high success rate (almost 100%) and can deliver the proteins in a timely fashion. This is a core competency necessary for efficient and effective production of immunogens and affinity ligands for antibody production and purification. GenWay has over 300

recombinant proteins

offered in our catalog.



GenWay Biotech, Inc. 6777 Nancy Ridge Drive, San Diego, CA 92121 Tel: (858)458-0866 Fax: (858)458-0833 Email: sales@genwaybio.com www.genwaybio.com ©1998 - 2010 GenWay Biotech, Inc. - All Rights Reserved