RELA antibody, Anti-NFKB p65 (Rel A) phospho specific pS276 Antibody, Rabbit N/A Antibody

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Anti-NFKB p65 (Rel A) phospho specific pS276 Antibody, Rabbit N/A Antibody

Catalog Number: 18-732-292083

Buy RELA antibody -

Volume:

Related Product Names:

- RELA antibody; RELA; NFKB3; Anti-NFKB p65 (Rel A) phospho specific pS276
- anti-NFkB p65 (Rel A) pS276 [Rabbit]; Nuclear factor NF-kappa-B p65 subunit; Transcription factor p65

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: RELA	Gene Name Synonym: NFKB3
Gi #: N/A	NCBI Acc #: NP_068810.2	Swiss Prot Acc #: Q04206
Length (aa): N/A	Mol. Weight (Da): 60219	Chrom Location: N/A

Antigen: NFKB p65 pS276

Immunogen: NFkB p65 (Rel A) peptide corresponding to a region near phospho Serine 276 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). Sequence information: QLRRPpSDRELSC

Family: NFkB Signaling

Fraction: Serum

Application Note: This phospho specific polyclonal antibody reacts human pS276 p65 and shows minimal reactivity by western blot with non-phosphorylated p65 and minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.

Preservative: 0.1% (w/v) Sodium Azide.

Recommended Dilution: For immunoblotting a 1:1,000 dilution is recommended. A 65 kDa band corresponding to human p65 is detected. HeLa cells or TNF inducible KBM-5 cells can be used as a positive control. For ELISA a 1:10,000 to 1:30,000 dilution is recommended. Researchers should determine optimal titers for other applications.

Physical State: Liquid (sterile filtered)

Purity and Specificity: This product was prepared from monospecific antiserum by delipidation and defibrination. This phospho specific polyclonal antibody is specific for phosphorylated pS276 human p65. Reactivity with non-phosphorylated p65 is minimal. Cross reactivity with pS276 phosphorylated p65 from mouse, rat or other species has not been determined.

Antibody Concentration: 90.0 mg/ml (by Refractometry)

Stabilizer: None

Ship Code: DRYICE

Source/Host: Rabbit

Purity/Purification: Monospecific Antiserum

Crossreactivity: Bovine, Human, Mouse, Rat, Sheep

Format: Liquid (sterile filtered)

Storage: -20C or Colder

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for RELA ANTIBODY:

IHC, ELISA, WB, IP: Tested

TESTING: (secondary reagents and protocols )

Not Available

RELA ANTIBODY TARGET DESCRIPTION:

Synonym Names for RELA antibody: RELA; NFKB3; anti-NFkB p65 (Rel A) pS276 [Rabbit]; Nuclear factor NF-kappa-B p65 subunit; Transcription factor p65

Function: NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex.

Subunit: Component of the NF-kappa-B p65-p50 complex. Component of the NF-kappa-B p65-c-Rel complex. Homodimer; component of the NF-kappa-B p65-p65 complex. Component of the NF-kappa-B p65-p52 complex. May interact with ETHE1. Binds AES and TLE1. Interacts with TP53BP2. Binds to and is phosphorylated by the activated form of either RPS6KA4 or RPS6KA5. Interacts with ING4 and this interaction may be indirect. Interacts with CARM1, USP48 and UNC5CL. Interacts with IRAK1BP1 (By similarity).Interacts with NFKBID (By similarity). Interacts with NFKBIA. Interacts with GSK3B. Interacts with NFKBIB (By similarity). Interacts with NFKBIE. Interacts with NFKBIZ (By similarity). Part of a 70-90 kDa complex at least consisting of CHUK, IKBKB, NFKBIA, RELA, IKBKAP and MAP3K14. Interacts with HDAC3; HDAC3 mediates the deacetylation of RELA. Interacts with HDAC1; the interaction requires non-phosphorylated RELA. Interacts with CBP; the interaction requires phosphorylated RELA. Interacts (phosphorylated at 'Thr-254') with PIN1; the interaction inhibits p65 binding to NFKBIA. Interacts with SOCS1. Interacts with UXT.

Subcellular Location: Nucleus. Cytoplasm. Note=Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).

Ptm: Ubiquitinated, leading to its proteosomal degradation. Degradation is required for termination of NF-kappa-B response.

Ptm: Phosphorylation on 'Ser-536' stimulates acetylation on 'Lys-310' and interaction with CBP; the phosphorylated and acetylated forms show enhanced transcriptional activity.

Ptm: Reversibly acetylated; the acetylation seems to be mediated by CBP, the deacetylation by HDAC3. Acetylation at 'Lys-122' enhances DNA binding and impairs association with NFKBIA. Acetylation at 'Lys-310' is required for full transcriptional activity in the absence of effects on DNA binding and NFKBIA association. Acetylation can also lower DNA-binding and results in nuclear export.

Similarity: Contains 1 RHD (Rel-like) domain.

Anti-NFKB p65 (Rel A) phospho specific pS276 reacts with bovine, human, mouse, rat, sheep.

OMIM: 164014; gene. [NCBI / EBI]

Pathways:
KEGG pathway: Adipocytokine signaling pathway 04920
KEGG pathway: Apoptosis 04210
KEGG pathway: Chronic myeloid leukemia 05220
KEGG pathway: Epithelial cell signaling in Helicobacter pylori infection 05120
KEGG pathway: Pancreatic cancer 05212
KEGG pathway: Prostate cancer 05215
KEGG pathway: Small cell lung cancer 05222
KEGG pathway: Toll-like receptor signaling pathway 04620
Reactome Event:Signaling in Immune system 168256
Reactome Event:Signalling by NGF 166520

Products similar to RELA antibody:

IgG

Placental Growth Factor [37203.111], IgG

C Reactive Protein [C7], IgG

NF-kappaB-p65 (Ab-311), IgG

NFkB p65 [NF-12], IgG

C Reactive Protein [C2], IgG

Peptide

NFkappaB-p65 (phospho-Ser311) Antibody Blocking Peptide, Peptide

NFkappaB-p65 (Phospho-Ser468) Antibody Blocking Peptide, Peptide

NFkappaB-p65 (Phospho-Thr254) Antibody Blocking Peptide, Peptide

NFkappaB-p65 (Phospho-Ser529) Antibody Blocking Peptide, Peptide

NFkappaB-p65 (Phospho-Thr435) Antibody Blocking Peptide, Peptide

PRODUCT INTERACTIONS:

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with AATF (apoptosis antagonizing transcription factor), IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with apoptosis antagonizing transcription factor, IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with AATF (apoptosis antagonizing transcription factor), IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with Anti-ATF3 (RABBIT), IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with Inhibitor of nuclear factor kappa-B kinase alpha subunit, IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with IKK, IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with IKK alpha [14A231], IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with IKK -alpha (Phospho-Thr23), IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with IKK -alpha (Ab-23), IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with IKK -alpha (Phospho-Thr23) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with Rabbit A' IKK-alpha, IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with IKK-alpha Control Peptide, Peptide (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with Anti-IKK alpha, IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with Anti-IKK alpha CONTROL PEPTIDE, IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with IKK alpha phospho S176, S180, IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with CREB BINDING PROTEIN, IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with CBP, IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with HDAC1 (histone deacetylase 1), IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with HDAC1 [HDAC1-21], IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with Anti-HDAC-1, IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with HDAC1, IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with HDAC1 (ready-to-use), IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with HDAC1, IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with NFKB1 (nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (p105)), IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with NFkB p50, IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with NFkB, IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with NFkB p50 (ready-to-use), IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with NF-kappaB-p105/p50 (Phospho-Ser932), IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with NF-kappaB-p105/p50 (Ab-932), IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with NF-kappaB-p105/p50 (Phospho-Ser337), IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with NF-kappaB-p105/p50 (Phospho-Ser893), IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with NF-kappaB-p105/p50 (Phospho-Ser907), IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with NF-kappaB-p105/p50 (Ab-337), IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with NF-kappaB-p105/p50 (Ab-893), IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with NF-kappaB-p105/p50 (Ab-907), IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with NFkappaB-p105/p50 (Phospho-Ser337) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with NFkappaB-p105/p50 (Phospho-Ser893) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with NFkappaB-p105/p50 (Phospho-Ser907) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with NFkappaB-p105/p50 Phospho-Ser932) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with Rabbit anti p50 (NFkB1) subunit, IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with Rabbit anti NFkB p105 subunit, IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with Anti-NFKB p50 (NFKB1), IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with Anti-NFKB p105, IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with NFkB p50, IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with NFkB, IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with NF KAPPA B INHIBITOR ALPHA, IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with NF KAPPA B INHIBITOR, IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with IKB alpha [6A920], IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with IKB alpha phospho S32, S36 [39A1431], IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with IKB alpha cleaved, IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with I-kappaB-alpha (Phospho-Tyr42), IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with I-kappaB-alpha (Ab-42), IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with I-kappaB-alpha (Ab-32/36), IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with IkappaB-alpha (Phospho-Ser32/Ser36) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with IkappaB-alpha (Phospho-Tyr42) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with NFKBIA, IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with Rabbit anti IkB-alpha (C-terminal), IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with IkB-alpha (C-terminal) Control Peptide, Peptide (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with Anti-IKB alpha (Human MOUSE Rat Specific) C-terminal, IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with IKB alpha, IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with IKB alpha phospho S32, S36, IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with NFKBIB (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor. beta), IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with Rabbit A' IkB-beta (C-terminal), IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with IkB-beta (C-terminal) Control Peptide, Peptide (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with Anti-IKB beta, IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with NFkB c-Rel, IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with Rabbit anti c-Rel subunit, IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with NFkB c-Rel subunit Control Peptide, Peptide (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with Anti-NFKB cRel, IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with c-Rel, IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with NFkB c Rel, IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with MSK1 phospho T581, IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with MSK1 (Ab-376), IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with TGaseII Human, Protein (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with t-TG Human, Protein (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with Transglutaminase 2 (ready-to-use), IgG (GenWay Catalog #: 18-732-292083).

Anti-NFKB p65 (Rel A) phospho specific pS276 interacts with Rabbit anti Transglutaminase C, IgG (GenWay Catalog #: 18-732-292083).

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

BACKGROUND REFERENCES for RELA ANTIBODY:

Background references for antibody target are not specific to GenWay products

Ashikawa K, Majumdar S, Banerjee S, Bharti AC, Shishodia S, and Aggarwal BB (2002) Piceatannol inhibits TNF-induced NF-kappaB activation and NF-kappaB-mediated gene expression through suppression of IkappaBalpha kinase and p65 phosphorylation. J Immunol 169-11: 6490-7.
Baldwin, A.S. (2001) Control of oncogenesis and cancer therapy resistance by the transcription factor NF-kB. J. Clinical Investigation 107: 241-246.
Baldwin, A.S., Jr. (2001) Series introduction: the transcription factor NF-kB and human disease. J. Clinical Investigation 107: 3-6.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: RELA antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of RELA antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about RELA antibody, all related information, articles, resources about RELA antibody are being stored on our online database. Let us know if you have questions regarding this product.

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