SUMO1 antibody, Anti-SUMO (Yeast) Antibody, Rabbit N/A Antibody

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Anti-SUMO (Yeast) Antibody, Rabbit N/A Antibody

Catalog Number: 18-732-292212

Buy SUMO1 antibody -

Size:

Related Product Names:

- SUMO1 antibody; SUMO1; SMT3C; SMT3H3; UBL1; Anti-SUMO (Yeast)
- IgG fraction of Anti-SUMO (Yeast) (Rabbit); SUMO-1; Sentrin; Ubiquitin-like protein SMT3C; SMT3 homolog 3; Ubiquitin-homology domain protein PIC1; Ubiquitin-like protein UBL1; GAP-modifying protein 1; GMP1; Small ubiquitin-related modifier 1

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: SUMO1	Gene Name Synonym: SMT3C; SMT3H3; UBL1
Gi #: N/A	NCBI Acc #: NP_001005781.1	Swiss Prot Acc #: P63165
Length (aa): N/A	Mol. Weight (Da): 11557	Chrom Location: N/A

Immunogen: This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast SUMO protein.

Family: Ubiquitin & UBL

Application Note: This purified polyclonal antibody reacts yeast SUMO by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.

Recommended Dilution: For immunoblotting a 1:1,000 dilution is recommended. A 12 kDa band corresponding to yeast SUMO is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation. For ELISA a 1:1,000 to 1:5,000 dilution is recommended. Researchers should determine optimal titers for other applications.

Physical State: Lyophilized

Purity and Specificity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Antibody Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01% (w/v) Sodium Azide

Source/Host: Rabbit

Purity/Purification: IgG Fraction

Crossreactivity: Yeast

Format: Liquid (sterile filtered)

Storage: Store vial at 4° C prior to restoration. Restore with 0.5 ml of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for SUMO1 ANTIBODY:

ELISA, WB, IP: Tested

TESTING: (secondary reagents and protocols )

Application ( anti-SUMO1 )

SUMO1 antibody licat image
SUMO1

SUMO1 ANTIBODY TARGET DESCRIPTION:

Synonym Names for SUMO1 antibody: SUMO1; SMT3C; SMT3H3; UBL1; IgG fraction of Anti-SUMO (Yeast) (Rabbit); SUMO-1; Sentrin; Ubiquitin-like protein SMT3C; SMT3 homolog 3; Ubiquitin-homology domain protein PIC1; Ubiquitin-like protein UBL1; GAP-modifying protein 1; GMP1; Small ubiquitin-related modifier 1

Covalent modification of cellular proteins by the ubiquitin-like modifier SUMO (small ubiquitin-like modifier) regulates various cellular processes, such as nuclear transport, signal transduction, stress responses and cell cycle progression. But, in contrast to ubiquination, sumoylation does not tag proteins for degradation by the 26S proteasome, but rather seems to enhance stability or modulate their subcellular compartmentalization. Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. Once covalently attached to cellular targets, SUMO regulates protein:protein and protein:DNA interactions, as well as localization and stability of the target protein. Sumoylation occurs in most eukaryotic systems, and SUMO is highly conserved from yeast to humans. Where invertebrates have only a single SUMO gene termed SMT3, three members of the SUMO family have been identified in vertebrates: SUMO-1 and the close homologues SUMO-2 and SUMO-3. SUMO has been called SMT3 (yeast), sentrin, PIC1, GMP1 and UBL1. SUMO has been shown to bind and regulate mammalian SP-RINGs (such as Mdm2, PIAS and PML), RanGAP1, RanBP2, p53, p73, HIPK2, TEL, c-Jun, Fas, Daxx, TNFRI, Topo-I, Topo-II, WRN, Sp100, I?B-?, Androgen receptor (AR), GLUT1/4, Drosophila Ttk69, Dorsal, CaMK, yeast Septins, and viral CMV-IE1/2, EBV-BZLF1, HPV/BPV-E1. These bindings implicate SUMO in the stabilization of the target proteins and/or their localization to subcellular complexes. SUMO has an apparent molecular weight of ~12kDa and human SUMO-1 (a 101 amino acid polypeptide) shares 50% sequence identity with SUMO-2 and SUMO-3 and with yeast SMT3. SUMO and ubiquitin only show about 18% homology, but both possess a common three-dimensional structure characterized by a tightly packed globular fold with ?- sheets wrapped around an ?-helix.

Function: Ubiquitin-like protein which can be covalently attached to target lysines as a monomer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4.

Subunit: Interacts with SAE2, UBE2I, RANBP2, PIAS1 and PIAS2. Interacts with PARK2. Covalently attached to a number of proteins such as PML, RANGAP1, HIPK2, SP100, p53, p73-alpha, MDM2, JUN, DNMT3B and TDG. Also interacts with HIF1A, HIPK2, HIPK3, CHD3, EXOSC9, RAD51 and RAD52.

Subcellular Location: Nucleus membrane. Nucleus speckle. Cytoplasm.

Ptm: Cleavage of precursor form by SENP1 or SENP2 is necessary for function.

Similarity: Belongs to the ubiquitin family. SUMO subfamily.

Similarity: Contains 1 ubiquitin-like domain.

Anti-SUMO (Yeast) reacts with yeast.

OMIM: 601912; gene. [NCBI / EBI]

Products similar to SUMO1 antibody:

IgG

SUMO-1, IgG

Anti-SUMO, IgG

SUMO-1, IgG

SUMO-I Human, Protein

PRODUCT INTERACTIONS:

Anti-SUMO (Yeast) interacts with DAXX, IgG (GenWay Catalog #: 18-732-292212).

Anti-SUMO (Yeast) interacts with DAXX [DAXX-01], IgG (GenWay Catalog #: 18-732-292212).

Anti-SUMO (Yeast) interacts with DAXX, IgG (GenWay Catalog #: 18-732-292212).

Anti-SUMO (Yeast) interacts with Ubc9 (Human ubiquitin conjugating enzyme 9), Protein (GenWay Catalog #: 18-732-292212).

Anti-SUMO (Yeast) interacts with Ubc9 Human His, Protein (GenWay Catalog #: 18-732-292212).

Anti-SUMO (Yeast) interacts with UbC9 Human, Protein (GenWay Catalog #: 18-732-292212).

Anti-SUMO (Yeast) interacts with UBE2I (ubiquitin-conjugating enzyme E2I (UBC9 homolog. yeast)), IgG (GenWay Catalog #: 18-732-292212).

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

BACKGROUND REFERENCES for SUMO1 ANTIBODY:

Background references for antibody target are not specific to GenWay products

Muller, S. , Hoege, C. , Pyrowolakis, G. and Jentsch, S. (2001) SUMO, ubiquitin's mysterious cousin. Nat Rev Mol Cell Biol, 2(3): 202-10.
Hochstrasser, M. (2001) SP-RING for SUMO: new functions bloom for a ubiquitin-like protein. Cell, 107(1): 5-8.
Kahyo, T.,Nishida, T. and Yasuda, H. (2001) Involvement of PIAS1 in the sumoylation of tumor suppressor p53. Mol Cell, 8(3) 713-8.
Takahashi, Y., Kahyo, T., Toh-E, A.,Yasuda, H. and Kikuchi, Y. (2001) Yeast Ull1/Siz1 is a novel SUMO1/Smt3 ligase for septin components and functions as an adaptor between conjugating enzyme and substrates. J Biol Chem 276(52): 48973-7.
Yeh, E.T., Gong, L. and Kamitani, T. (2000) Ubiquitin-like proteins: new wines in new bottles. Gene, 248(1-2):1-14.
Keane, M.M., Ettenberg, S.A., Nau, M.M., Banerjee, P., Cuello, M., Penninger, J., and Lipkowitz, S. (1999) cbl-3: a new mammalian cbl family protein. Oncogene, 18(22):3365-75.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: SUMO1 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of SUMO1 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about SUMO1 antibody, all related information, articles, resources about SUMO1 antibody are being stored on our online database. Let us know if you have questions regarding this product.

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