COL4A1 antibody, Anti-Collagen Type IV Antibody, Rabbit N/A Antibody

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Anti-Collagen Type IV Antibody, Rabbit N/A Antibody

Catalog Number: 18-732-292345

Buy COL4A1 antibody -

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Related Product Names:

- COL4A1 antibody; COL4A1; Anti-Collagen Type IV
- Affinity Purified Anti-Collagen Type IV [Rabbit] (Minimum Cross Reactivity to Type I, II, III, V and VI Collagens); Arresten; Collagen alpha-1(IV) chain

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: COL4A1	Gene Name Synonym: N/A
Gi #: N/A	NCBI Acc #: NP_001836.2	Swiss Prot Acc #: P02462
Length (aa): N/A	Mol. Weight (Da): 160615	Chrom Location: N/A

Immunogen: Collagen Type IV from human and bovine placenta

Family: Extracellular Matrix

Application: Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for native (non-denaturing, non-dissociating) PAGE and western blotting for highly sensitive qualitative analysis.

Recommended Dilution: This product was assayed by immunoblot and found to be reactive against Collagen IV at a dilution of 1:5,000 to 1:10,000. This product was also assayed against 1.0 ug of Collagen IV in a direct ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) (code #611-1302) and (ABTS (2,2'-azino-bis-[3- ethylbenthiazoline-6-sulfonic acid]) (code # ABTS-100) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:8,000 of the stock concentration is suggested for this product. For immunohistochemistry on frozen tissue sections, dilute the product 1:50 to 1:200. Optimal titers for other applications should be determined by the researcher.

Physical State: Liquid (sterile filtered)

Purity and Specificity: This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Antibody Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA; pH 8.0

Stabilizer: None

Source/Host: Rabbit

Purity/Purification: Affinity Purified

Crossreactivity: Human, Bovine, Mouse, Rat

Format: Liquid (sterile filtered)

Storage: -20C or Colder

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for COL4A1 ANTIBODY:

IHC, ELISA, WB, IP: Tested

TESTING: (secondary reagents and protocols )

Not Available

COL4A1 ANTIBODY TARGET DESCRIPTION:

Synonym Names for COL4A1 antibody: COL4A1; Affinity Purified Anti-Collagen Type IV [Rabbit] (Minimum Cross Reactivity to Type I, II, III, V and VI Collagens); Arresten; Collagen alpha-1(IV) chain

Rockland produces highly active antibodies and conjugates to collagens. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of ‘type’ specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDSPAGE and immunoblotting.

Function: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Potently inhibits endothelial cell proliferation and angiogenesis. Inhibits angiogenesis potentially via mechanisms involving cell surface proteoglycans and the alpha and beta integrins of endothelial cells.

Subunit: There are six type IV collagen isoforms, alpha 1(IV)-alpha 6(IV), each of which can form a triple helix structure with 2 other chains to generate type IV collagen network.

Subcellular Location: Secreted, extracellular space, extracellular matrix, basement membrane.

Tissue Specificity: Highly expressed in placenta.

Domain: Alpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain.

Ptm: Lysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in all cases and bind carbohydrates.

Ptm: Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

Ptm: Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.

Ptm: The trimeric structure of the NC1 domains may be stabilized by covalent bonds between Lys and Met residues.

Disease: Defects in COL4A1 are a cause of brain small vessel disease with hemorrhage [MIM:607595]. Brain small vessel diseases underlie 20 to 30 percent of ischemic strokes and a larger proportion of intracerebral hemorrhages. Inheritance is autosomal dominant.

Disease: Defects in COL4A1 are the cause of hereditary angiopathy with nephropathy, aneurysms, and muscle cramps (HANAC) [MIM:611773]. The clinical renal manifestations include hematuria and bilateral large cysts. Histologic analysis revealed complex basement membrane defects in kidney and skin. The systemic angiopathy appears to affect both small vessels and large arteries.

Disease: Defects in COL4A1 are a cause of porencephaly type 1 [MIM:175780]; also known as encephaloclastic porencephaly. Porencephaly is a term used for any cavitation or cerebrospinal fluid-filled cyst in the brain. Porencephaly type 1 is usually unilateral and results from focal destructive lesions such as fetal vascular occlusion or birth trauma. Inheritance is autosomal dominant.

Similarity: Belongs to the type IV collagen family.

Similarity: Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain.

Anti-Collagen Type IV reacts with human, bovine, mouse, rat.

OMIM: 120130; gene. [NCBI / EBI]
175780; phenotype. [NCBI / EBI]
607595; phenotype. [NCBI / EBI]
611773; phenotype. [NCBI / EBI]

Pathways:
KEGG pathway: Cell Communication 01430
KEGG pathway: ECM-receptor interaction 04512
KEGG pathway: Focal adhesion 04510
KEGG pathway: Small cell lung cancer 05222

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SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

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Precautions: COL4A1 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of COL4A1 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about COL4A1 antibody, all related information, articles, resources about COL4A1 antibody are being stored on our online database. Let us know if you have questions regarding this product.

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