Alphabetical Listing of Primary Antibodies:
f
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0-4
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5-9
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A
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B
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C
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D
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E
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F
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G
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H
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I
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J
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K
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L
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M
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N
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O
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P
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Q
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R
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S
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T
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U
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V
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W
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X
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Y
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Z
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Alphabetical Listing of Proteins:
f
|
0-4
|
5-9
|
A
|
B
|
C
|
D
|
E
|
F
|
G
|
H
|
I
|
J
|
K
|
L
|
M
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N
|
O
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P
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Q
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R
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S
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T
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U
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V
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W
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X
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Y
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Z
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Peptides
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Secondary Antibodies
|
ELISA Kits
Anti-Collagen Type V Antibody, Rabbit N/A Antibody
Catalog Number: 18-732-292346
Buy COL5A1 antibody -
Size:
0.1 mg ($330.00)
Related Product Names:
- COL5A1 antibody; COL5A1; Anti-Collagen Type V
- Affinity Purified Anti-Collagen Type V [Rabbit] Minimum Cross Reactivity to Type I, II, III, IV and VI Collagens; N/A; Collagen alpha-1(V) chain
- Gene Information -
Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact
sales@genwaybio.com
.
Gene Name:
COL5A1
Gene Name Synonym:
N/A
Gi #:
N/A
NCBI Acc #:
NP_000084.3
Swiss Prot Acc #:
P20908
Length (aa):
N/A
Mol. Weight (Da):
183560
Chrom Location:
N/A
Family:
Extracellular Matrix
Conjugation Reference:
Collagen Type V from human and bovine placenta
Application:
Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for native (non-denaturing, non-dissociating) PAGE and western blotting for highly sensitive qualitative analysis.
Recommended Dilution:
ELISA : 1:5,000 - 1:10,000
WESTERN BLOT : 1:5,000 - 1:10,000
IMMUNOHISTOCHEMISTRY : 1:50 - 1:200
OTHER APPLICATIONS : User Optimized
Physical State:
Liquid (sterile filtered)
Purity and Specificity:
This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type V collagens and has negligible cross-reactivity with Type I, II, III, IV or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Antibody Concentration:
1.1 mg/ml (by UV absorbance at 280 nm)
Buffer:
0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA; pH 8.0
Stabilizer:
None
Source/Host:
Rabbit
Purity/Purification:
Affinity Purified
Crossreactivity:
Human, Bovine, Mouse, Rat
Format:
Liquid (sterile filtered)
Storage:
-20C or Colder
Shipping:
Products may be shipped on ice pack or dry ice.
APPLICATIONS for COL5A1 ANTIBODY:
IHC, ELISA, WB, IP: Tested
TESTING: (
secondary reagents
and
protocols
)
Not Available
COL5A1 ANTIBODY TARGET DESCRIPTION:
Synonym Names for COL5A1 antibody:
COL5A1; Affinity Purified Anti-Collagen Type V [Rabbit] Minimum Cross Reactivity to Type I, II, III, IV and VI Collagens; N/A; Collagen alpha-1(V) chain
Rockland produces highly active antibodies and conjugates to collagens. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.
Function:
Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
Subunit:
Trimers of two alpha 1(V) and one alpha 2(V) chains in most tissues and trimers of one alpha 1(V), one alpha 2(V), and one alpha 3(V) chains in placenta. Interacts with CSPG4.
Subcellular Location:
Secreted, extracellular space, extracellular matrix (By similarity).
Ptm:
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Ptm:
Sulfated on 40% of tyrosines.
Disease:
Defects in COL5A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
Disease:
Defects in COL5A1 are a cause of Ehlers-Danlos syndrome type 2 (EDS2) [MIM:130010]; also known as Ehlers-Danlos syndrome mitis.
Similarity:
Belongs to the fibrillar collagen family.
Similarity:
Contains 1 laminin G-like domain.
Similarity:
Contains 1 TSP N-terminal (TSPN) domain.
Anti-Collagen Type V reacts with human, bovine, mouse, rat.
OMIM:
120215; gene. [
NCBI
/
EBI
]
130000; phenotype. [
NCBI
/
EBI
]
130010; phenotype. [
NCBI
/
EBI
]
Pathways:
KEGG pathway: Cell Communication
01430
KEGG pathway: ECM-receptor interaction
04512
KEGG pathway:
Focal adhesion
04510
Products similar to COL5A1 antibody:
IgG
Collagen V [MC5-HA], IgG
SUGGESTED ANITBODY REAGENTS
- GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.
Order Confirmation:
Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.
Precautions:
COL5A1 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.
Important Notes:
During shipment, small volumes of COL5A1 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.
Copyright:
This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about COL5A1 antibody, all related information, articles, resources about COL5A1 antibody are being stored on our online database. Let us know if you have questions regarding this product.
Disclaimer:
For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed.
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