ATF3 antibody, Anti-ATF3 (RABBIT) Antibody, Rabbit N/A Antibody

Alphabetical Listing of Primary Antibodies:	f\| 0-4 \| 5-9 \| A \| B \| C \| D \| E \| F \| G \| H \| I \| J \| K \| L \| M \| N \| O \| P \| Q \| R \| S \| T \| U \| V \| W \| X \| Y \| Z \|
Alphabetical Listing of Proteins:	f\| 0-4 \| 5-9 \| A \| B \| C \| D \| E \| F \| G \| H \| I \| J \| K \| L \| M \| N \| O \| P \| Q \| R \| S \| T \| U \| V \| W \| X \| Y \| Z \|
Peptides \| Secondary Antibodies \| ELISA Kits

Anti-ATF3 (RABBIT) Antibody, Rabbit N/A Antibody

Catalog Number: 18-732-292437

Buy ATF3 antibody -

Size:

Related Product Names:

- ATF3 antibody; ATF3; Anti-ATF3 (RABBIT)
- Affinity Purified anti-ATF3 [Rabbit]; Activating transcription factor 3; Cyclic AMP-dependent transcription factor ATF-3

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: ATF3	Gene Name Synonym: N/A
Gi #: N/A	NCBI Acc #: NP_001025458.1	Swiss Prot Acc #: P18847
Length (aa): N/A	Mol. Weight (Da): 20576	Chrom Location: N/A

Antigen: Human ATF3

Immunogen: This antibody was produced from a synthetic peptide corresponding to aa 113-130 of human ATF3.

Preservative: 0.01% (w/v) Sodium Azide.

Family: Transcription Factor

Fraction: Affinity Purified IgG

Application Note: Affinity purified anti-ATF3 has been tested by ELISA and western blotting against recombinant forms of the protein. Although not tested, this antibody is likely function in most immunoassays including immunofluorescence microscopy, immunohistochemistry.

Recommended Dilution: This product was assayed by immunoblot and was found to be reactive against recombinant ATF3 present in transfected mammalian or E.coli whole cell extracts. For western blot a 1:200 dilution yields strong signal with relatively clean background staining. Use IRDYE800 Conjugated Affinity Purified Anti-RABBIT IgG (H&L) (GOAT) Min X Bv Ch Gt GP Hs Hu Ms Rt & Sh Serum Proteins (code 611-132- 122) as a secondary antibody for detection at a 1:5,000 dilution. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. For ELISA a 1:5,000 to 1:20,000 dilution gave strong signal against the immunizing peptide. Similar dilutions should generate sufficient ELISA signal against native or recombinant ATF3 protein. The researcher should determine optimal titers for other applications.

Physical State: Liquid (sterile filtered)

Purity and Specificity: This affinity-purified antibody is directed against human ATF3 and is useful in determining its presence in various assays including ELISA and western blotting. This polyclonal antibody recognizes over-expressed ATF3 protein found in various expression systems. Reactivity is observed against human ATF3. Cross reactivity with ATF3 from other mammalian sources has not been tested.

Antibody Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Ship Code: Dry Ice

Source/Host: Rabbit

Purity/Purification: Affinity Purified

Crossreactivity: Human, Bovine, Mouse, Rat

Format: Liquid (sterile filtered)

Storage: Store vial at -20C. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use. Expiration date is One (1) year from date of restoration.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for ATF3 ANTIBODY:

ELISA, WB, IP: Tested

TESTING: (secondary reagents and protocols )

Application ( anti-ATF3 )

ATF3 antibody licat image
ATF3

ATF3 ANTIBODY TARGET DESCRIPTION:

Synonym Names for ATF3 antibody: ATF3; Affinity Purified anti-ATF3 [Rabbit]; Activating transcription factor 3; Cyclic AMP-dependent transcription factor ATF-3

ATF3, or Activating Transcription Factor 3, is a member of mammalian activation TF/CREB protein family of transcription factors. ATF3 binds the cAMP response element (cre) (consensus: 5'-gtgacgt[ac][ag]-3'), a sequence present in many viral and cellular promoters. However, ATF3 represses rather than activates transcription from promoters with ATF sites stabilizing inhibitory co-factors at the promoter. Alternate splicing forms of ATF3, called ATF3 delta Zip, lack the leucine zipper domain and do not bind DNA. ATF3 delta Zip stimulates transcription, presumably by sequestering inhibitory co-factors away from the promoter. Human ATF3 (SwissProt 18847) is a 20575 Da protein composed of 181 amino acids.

Function: This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.

Subunit: Binds DNA as a homodimer or a heterodimer.

Subcellular Location: Nucleus.

Similarity: Belongs to the bZIP family. ATF subfamily.

Similarity: Contains 1 bZIP domain.

Anti-ATF3 (RABBIT) reacts with human, bovine, mouse, rat.

OMIM: 603148; gene. [NCBI / EBI]

PRODUCT INTERACTIONS:

Anti-ATF3 (RABBIT) interacts with FHL2 (four and a half LIM domains 2), IgG (GenWay Catalog #: 18-732-292437).

Anti-ATF3 (RABBIT) interacts with FHL2, IgG (GenWay Catalog #: 18-732-292437).

Anti-ATF3 (RABBIT) interacts with RELA, IgG (GenWay Catalog #: 18-732-292437).

Anti-ATF3 (RABBIT) interacts with NFkB p65, IgG (GenWay Catalog #: 18-732-292437).

Anti-ATF3 (RABBIT) interacts with NF-kappaB-p65 (Phospho-Ser529), IgG (GenWay Catalog #: 18-732-292437).

Anti-ATF3 (RABBIT) interacts with NF-kappaB-p65 (Ab-529), IgG (GenWay Catalog #: 18-732-292437).

Anti-ATF3 (RABBIT) interacts with NF-kappaB-p65 ( (phospho-Ser311), IgG (GenWay Catalog #: 18-732-292437).

Anti-ATF3 (RABBIT) interacts with NF-kappaB-p65 (Ab-311), IgG (GenWay Catalog #: 18-732-292437).

Anti-ATF3 (RABBIT) interacts with NF-kappaB-p65 (Phospho-Thr254), IgG (GenWay Catalog #: 18-732-292437).

Anti-ATF3 (RABBIT) interacts with NF-kappaB-p65 (Phospho-Ser276), IgG (GenWay Catalog #: 18-732-292437).

Anti-ATF3 (RABBIT) interacts with NF-kappaB-p65 (Phospho-Thr435), IgG (GenWay Catalog #: 18-732-292437).

Anti-ATF3 (RABBIT) interacts with NF-kappaB-p65 (Phospho-Ser468), IgG (GenWay Catalog #: 18-732-292437).

Anti-ATF3 (RABBIT) interacts with NF-kappaB-p65 (Phospho-Ser536), IgG (GenWay Catalog #: 18-732-292437).

Anti-ATF3 (RABBIT) interacts with NF-kappaB-p65 (Ab-254), IgG (GenWay Catalog #: 18-732-292437).

Anti-ATF3 (RABBIT) interacts with NF-kappaB-p65 (Ab-276), IgG (GenWay Catalog #: 18-732-292437).

Anti-ATF3 (RABBIT) interacts with NF-kappaB -p65 (Ab-435), IgG (GenWay Catalog #: 18-732-292437).

Anti-ATF3 (RABBIT) interacts with NF-kappaB-p65 (Ab-468), IgG (GenWay Catalog #: 18-732-292437).

Anti-ATF3 (RABBIT) interacts with NF-kappaB-p65 (Ab-536), IgG (GenWay Catalog #: 18-732-292437).

Anti-ATF3 (RABBIT) interacts with NFkappaB-p65 (Phospho-Thr254) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 18-732-292437).

Anti-ATF3 (RABBIT) interacts with NFkappaB-p65 (Phospho-Ser276) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 18-732-292437).

Anti-ATF3 (RABBIT) interacts with NFkappaB-p65 (Phospho-Thr435) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 18-732-292437).

Anti-ATF3 (RABBIT) interacts with NFkappaB-p65 (Phospho-Ser468) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 18-732-292437).

Anti-ATF3 (RABBIT) interacts with NFkappaB-p65 (Phospho-Ser536) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 18-732-292437).

Anti-ATF3 (RABBIT) interacts with NFkappaB-p65 (Phospho-Ser529) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 18-732-292437).

Anti-ATF3 (RABBIT) interacts with NFkappaB-p65 (phospho-Ser311) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 18-732-292437).

Anti-ATF3 (RABBIT) interacts with Rabbit anti p65 (RelA) N-terminal, IgG (GenWay Catalog #: 18-732-292437).

Anti-ATF3 (RABBIT) interacts with Rabbit anti p65 (RelA) C-terminal, IgG (GenWay Catalog #: 18-732-292437).

Anti-ATF3 (RABBIT) interacts with Anti-NFKB p65 (Rel A) phospho specific pS276, IgG (GenWay Catalog #: 18-732-292437).

Anti-ATF3 (RABBIT) interacts with Anti-NFKB p65 (Rel A) phospho specific pS529, IgG (GenWay Catalog #: 18-732-292437).

Anti-ATF3 (RABBIT) interacts with Anti-NFKB p65 (Rel A), IgG (GenWay Catalog #: 18-732-292437).

Anti-ATF3 (RABBIT) interacts with Anti-NFKB p65 (Rel A) N-TERMINAL SPECIFIC, IgG (GenWay Catalog #: 18-732-292437).

Anti-ATF3 (RABBIT) interacts with Anti-NFKB p65 (Rel A) phospho specific pS536, IgG (GenWay Catalog #: 18-732-292437).

Anti-ATF3 (RABBIT) interacts with Anti-NFKB p65 NLS specific, IgG (GenWay Catalog #: 18-732-292437).

Anti-ATF3 (RABBIT) interacts with NFkB p65 [NF-12], IgG (GenWay Catalog #: 18-732-292437).

Anti-ATF3 (RABBIT) interacts with NFkB p65, IgG (GenWay Catalog #: 18-732-292437).

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

BACKGROUND REFERENCES for ATF3 ANTIBODY:

Background references for antibody target are not specific to GenWay products

Kool J, Hamdi M, Cornelissen-Steijger P, van der Eb AJ, Terleth C, van Dam H. (2003) Induction of ATF3 by ionizing radiation is mediated via a signaling pathway that includes ATM, Nibrin1, stress-induced MAPkinases and ATF-2.Oncogene 2003 Jul 3;22(27):4235-42.
Fan F, Jin S, Amundson SA, Tong T, Fan W, Zhao H, Zhu X, Mazzacurati L, Li X, Petrik KL, Fornace AJ Jr, Rajasekaran B, Zhan Q. (2002) ATF3 induction following DNA damage is regulated by distinct signaling pathways and over-expression of ATF3 protein suppresses cells growth. Oncogene 2002 Oct 24;21(49):7488-96.
T. Hai, C.D. Wolfgang, D.K. Marsee, A.E. Allen, and U. Sivaprasad. (1999) ATF3 and stress responses, Gene Expression 7:321-335. B.P.C. Chen, C.D. Wolfgang and T. Hai. (1996) Analysis of ATF3: a transcription factor induced by physiological stresses and modulated by gadd153/Chop10. Mol. Cell. Biol. 16:1157-1168.
Chen,B.P.C.; Liang,G.; Whelan,J.; Hai,T. (1994) ATF3 and ATF3 delta Zip. Transcriptional repression versus activation by alternatively spliced isoforms. J. Biol. Chem. 269:15819.
Hai,T.; Liu,F.; Coukos,W.J.; Green,M.R.; (1989) Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers. Genes Dev. 3:2083.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: ATF3 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of ATF3 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about ATF3 antibody, all related information, articles, resources about ATF3 antibody are being stored on our online database. Let us know if you have questions regarding this product.

Disclaimer: For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed.

GenWay is a Protein and Antibody Solutions Provider. Customer Satisfaction is Our Top Priority.

Thank you for visiting GenWay Biotech's Datasheet. GenWay is the world's leader in chicken, IgY antibody production, producing over 900 quality polyclonal IgY antibodies to date. From our unique recombinant protein production, utilizing a variety of protein expression systems, GenWay's polyclonal IgY antibodies incorporate more epitopes, increasing the success rate for ELISA, western blot, immunohistochemistry (IHC), immunocytochemistry (ICC), and many more immunoassays.



GenWay Biotech, Inc. 6777 Nancy Ridge Drive, San Diego, CA 92121 Tel: (858)458-0866 Fax: (858)458-0833 Email: sales@genwaybio.com www.genwaybio.com ©1998 - 2010 GenWay Biotech, Inc. - All Rights Reserved