|
|
| IRDye® 800CW Conjugated Affinity Purified Anti-HUMAN IgG F(c) (GOAT) Secondary Antibody |
 |
|
|
 |
| Catalog Number: 25-732-294136 |
|
|
|
| Related Product Names:
|
-
IGHG1 secondary antibody; IGHG1; IRDye® 800CW Conjugated Affinity Purified Anti-HUMAN IgG F(c) (GOAT)
- IRDye800CW Conjugated Affinity Purified anti-Human IgG F(c) [Goat]; N/A; Ig gamma-1 chain C region |
|
- Gene Information -
Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.
|
|
| Gene Name: IGHG1 |
Gene Name Synonym: N/A |
Gi #:
N/A |
NCBI Acc #:
N/A |
Swiss Prot Acc #:
P01857 |
Length (aa): N/A |
Mol. Weight (Da): 36106 |
Chrom Location: N/A |
|
|
 Antigen: F(c)
Immunogen: Human IgG F(c) fragment
Buffer: 0.02% M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
Preservative: 0.01% (w/v) Soidum Azide.
Family: Affinity Purified
Fraction: Affinity Purified IgG
Purity Note: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against Human IgG F(ab')2.
Conjugation: IRDYE800CW
Conjugation Reference: LI-COR Biosciences, Lincoln, NE.
Application: Fluorescence technology is widely used to detect proteins. However, many common visible fluorophores often result inconsiderable background fluorescence in the visible range. Visible fluorophores are rarely used for membrane-based protein detectionbecause of this high background. IRDye800 antibody and reagent conjugates are specifically designed for protein detection methods thatuse longer-wavelength, near-infrared (IR) fluorophores to visualize proteins in western blotting and other applications. Very low backgroundfluorescence in the IR range provides for a much higher signal-to-noise ratio than visible fluorophores. Detection levels in the picogram rangeon Western blots rival the sensitivity of chemiluminescence on film. IRDye800 conjugates are optimized for the Odyssey® Infrared ImagingSystem developed by LI-COR. IRDye800 conjugates are also suitable for immunofluorescence microscopy using commercially availableexcitation/emission filters in the 780nm/820nm range. Dual simultaneous labeling in western blots or microscopy is achieved whenIRDye800 conjugates are used in conjunction with Cy5.5 conjugates. IRDye800 conjugates provide an ultra-sensitive and convenientalternative to standard chemiluminescent protein detection methods, as well as a valuable tool for multicolor imaging.
IRDye800CW conjugates are specifically designed for LI-COR’s In-Cell Western assay for the Odyssey® Infrared Imaging System.IRDye800CW conjugates can be used for western blotting applications, but the fluorochrome/protein ratio is not optimal for this application sodetection sensitivity may be reduced. The In-Cell Western assay is a faster method for analyzing signal transduction pathways. In less timethan a gel-based Western, you can quantify proteins in a 96- or 384-well microplate. In-Cell Westerns use infrared dye-labeled secondaryantibodies to directly label proteins in fixed cultured cells, and quantify total fluorescence from each well. Time consuming and error-pronesteps such as lysate preparation, gel loading and electrophoresis, and membrane transfer are eliminated with In-Cell Westerns.Simultaneous, two-target detection enables quantitative and accurate measurement of abundance or phosphorylation of one target bynormalization against another target. Near-infrared probes yield high sensitivity for measuring small changes in protein amount or modification.Direct detection of proteins in their cellular context eliminates artifacts caused by cell lysis. The fast, microplate-based assay eliminateslysates, gels, and membranes required for conventional Western blots. The In-Cell Western assay is a moderate throughput method, ideal forscreening cell treatments or drug candidates for their effects on target proteins. Recommended plates for In-Cell Westerns are Nunc-Nalgene(p/n 167008) for 96-well format and Falcon (BD Biosciences p/n 353961) for 384-well format. Primary and secondary antibodies should bevalidated by Odyssey western blotting before performing the In-Cell Western assay to confirm specificity and performance of the reagent in theinfrared system.
Recommended Dilution: This product was tested by the In-Cell Western assay using A431 cells and Human host primary antibody. A 1:800 dilution is sufficient to detect signal in the In-Cell Western assay. Researchers should determine optimal titers for their targets and cell types.
Physical State: Lyophilized
Fluorochrome/Protein Ratio: 2.0 moles IRDye800CW/mole of Goat IgG
Emission Wavelength: 800 nm
Absorption Wavelength: 774 nm (in PBS)
Antibody Concentration: Lot specific and is stated on the vial
Label: IRDye800CW (MW 1166.2)
Stabilizer: 10 mg/ml BSA IgG and Protease free
Source/Host: Goat
Purity/Purification: Affinity Purified
Crossreactivity: Human IgG F(c)
Format: Lyophilized
Storage: Store vial at 4C prior to restoration. Restore with 0.5ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one(1) year from date of restoration.
Shipping: Products may be shipped on ice pack or dry ice. |
|
| APPLICATIONS for IGHG1 SECONDARY ANTIBODY: |
| WB, ELISA, IHC, Fluorescence Microscopy, FACS: Tested |
|
|
|
|
| IGHG1 SECONDARY ANTIBODY TARGET DESCRIPTION: |
Synonym Names for IGHG1 secondary antibody: IGHG1; IRDye800CW Conjugated Affinity Purified anti-Human IgG F(c) [Goat]; N/A; Ig gamma-1 chain C region
Subcellular Location: Secreted.
Disease: Chromosomal aberrations involving IGHG1 may be a cause of multiple myeloma [MIM:254500]. Translocation t(11;14)(q13;q32) with CCND1; translocation t(4;14)(p16.3;q32.3) with FGFR3; translocation t(6;14)(p25;q32) with IRF4.
Miscellaneous: Nie has the G1M(17) allotypic marker, 97-K, and the G1M(1) markers, 239-D and 241-L. KOL and EU sequences have the G1M(3) marker and the G1M (non-1) markers.
Miscellaneous: Nie also differs in the amidation states of 35, 116, 198, 269 and 272.
Miscellaneous: EU also differs in the amidation states of residues 155, 166, 177, 195, 198, 269, and 272 and in the order of residues 268-272.
Miscellaneous: KOL also differs in the amidation states of residues 198, 267 and 272.
IRDye® 800CW Conjugated Affinity Purified Anti-HUMAN IgG F(c) (GOAT) reacts with human igg f(c).
OMIM: 147100; gene. [NCBI / EBI]
254500; phenotype. [NCBI / EBI]
Products similar to IGHG1 secondary antibody:
IgG
 Human lgG (3E8), IgG
|
|
| PRODUCT INTERACTIONS: |
IRDye® 800CW Conjugated Affinity Purified Anti-HUMAN IgG F(c) (GOAT) interacts with Mouse CD32/CD16, IgG (GenWay Catalog #: 25-732-294136).
IRDye® 800CW Conjugated Affinity Purified Anti-HUMAN IgG F(c) (GOAT) interacts with Mouse CD32/CD16, IgG (GenWay Catalog #: 25-732-294136).
IRDye® 800CW Conjugated Affinity Purified Anti-HUMAN IgG F(c) (GOAT) interacts with CD32, IgG (GenWay Catalog #: 25-732-294136).
IRDye® 800CW Conjugated Affinity Purified Anti-HUMAN IgG F(c) (GOAT) interacts with Fc gamma RIIb (CD32) Phospho (pY292), IgG (GenWay Catalog #: 25-732-294136).
IRDye® 800CW Conjugated Affinity Purified Anti-HUMAN IgG F(c) (GOAT) interacts with Fc gamma RIIb (C-term), IgG (GenWay Catalog #: 25-732-294136).
|
|
Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.
Precautions: IGHG1 secondary antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.
Important Notes: During shipment, small volumes of IGHG1 secondary antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.
Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about IGHG1 secondary antibody, all related information, articles, resources about IGHG1 secondary antibody are being stored on our online database. Let us know if you have questions regarding this product.
Disclaimer: For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed. |
|
|
|
GenWay is a Protein and Antibody Solutions Provider. Customer Satisfaction is Our Top Priority.
|
|
|
|
|
|
