ADIPOQ protein, Adiponectin; globular domain) recombinant protein

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Peptides \| Secondary Antibodies \| ELISA Kits

Adiponectin; globular domain) recombinant protein

Catalog Number: 10-002-38002

Buy ADIPOQ protein -

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Related Product Names:

- ADIPOQ protein; ADIPOQ; ACDC; ACRP30; APM1; GBP28; Adiponectin; globular domain)
- Adiponectin (111-247aa; globular domain), Mouse, Recombinant, expressed in E Coli; Adipocyte, C1q and collagen domain-containing protein; 30 kDa adipocyte complement-related protein; Adipocyte complement-related 30 kDa protein; ACRP30; Adipose most abunda; Adiponectin

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: ADIPOQ	Gene Name Synonym: ACDC; ACRP30; APM1; GBP28
Gi #: N/A	NCBI Acc #: NP_004788.1	Swiss Prot Acc #: Q15848
Length (aa): N/A	Mol. Weight (Da): 26414	Chrom Location: N/A

Sequence: MAYMYRSAFS VGLETRVTVP NVPIRFTKIF YNQQNHYDGS TGKFYCNIPG LYYFSYHITV YMKDVKVSLF KKDKAVLFTY DQYQEKNVDQ ASGSVLLHLE VGDQVWLQVY GDGDHNGLYA 1015255010152550 14% SDS-PAGE DNVNDSTFTG FLLYHDTN

Concentration: 1mg/ml

Purity/Purification: >= 95% by SDS PAGE

Format: Liquid. In 20mM Tris-HCl pH7.5, 50mM NaCl, 5mM DTT, 10% Glycerol.

Storage: Store at -20C.

Shipping: Products may be shipped on ice pack or dry ice.

TESTING: (secondary reagents and protocols )

WB ( anti-ADIPOQ protein )

ADIPOQ protein image
ADIPOQ

ADIPOQ PROTEIN TARGET DESCRIPTION:

Synonym Names for ADIPOQ protein: ADIPOQ; ACDC; ACRP30; APM1; GBP28; Adiponectin (111-247aa; globular domain), Mouse, Recombinant, expressed in E Coli; Adipocyte, C1q and collagen domain-containing protein; 30 kDa adipocyte complement-related protein; Adipocyte complement-related 30 kDa protein; ACRP30; Adipose most abunda; Adiponectin

Adiponectin/Acrp30 (247amino acids) is adipocyte complement-related protein of 30kDa and exclusively expressed in differentiated adipocytes. Adiponectin (Acrp30) is a member of the complement factor C1q family and consists of signal sequence, Non-homologous sequence, collagen domain and globular domain (gAcrp30). Adiponectin (Acrp30) expression is reduced in a variety of obese and insulin-resistant states in human, monkeys and mice. Injection of Acrp30 (247aa) or gAcrp30 (globular domain) lowers serum glucose and free fatty acid level in mice. The globular domain of adiponectin/acrp30 (amino acid residues, 111-247, gAcrp30) was overexpressed in E.coli and purified by using conventional chromatography techniques.

Function: Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.

Subunit: Homomultimer. Forms trimers, hexamers and 12- to 18-mers. The trimers (low molecular weight complexes / LMW) are assembled via non-covalent interactions of the collagen-like domains in a triple helix and hydrophobic interactions within the globular C1q domain. Several trimers can associate to form disulfide-linked hexamers (middle molecular weight complexes / MMW) and larger complexes (higher molecular weight / HMW). The HMW-complex assembly may rely aditionnally on lysine hydroxylation and glycosylation. LMW, MMW and HMW complexes bind to HBEGF, MMW and HMW complexes bind to PDGFB, and HMW complex binds to FGF2.

Subcellular Location: Secreted.

Tissue Specificity: Synthesized exclusively by adipocytes and secreted into plasma.

Domain: The C1q domain is commonly called the globular domain.

Ptm: Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.

Ptm: HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes (By similarity).

Ptm: O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups.

Ptm: Not N-glycosylated.

Disease: Defects in ADIPOQ are the cause of adiponectin deficiency [MIM:605441]. The result is a very low concentration of plasma adiponectin. Decreased adiponectin plasma levels are associated with obesity insulin resistance, and diabetes type 2.

Pharmaceutical: Adiponectin might be used in the treatment of diabetes type 2 and insulin resistance.

Miscellaneous: Variants Arg-84 and Ser-90 show impaired formation of HMW complexes whereas variants Cys-112 and Thr-164 show impaired secretion of adiponectin in any form.

Miscellaneous: HMW-complex blood contents are higher in females than in males, are increased in males by castration and decreased again upon subsequent testosterone treatment, which blocks HMW-complex secretion (By similarity). In type 2 diabetic patients, both the ratios of HMW to total adiponectin and the degree of adiponectin glycosylation are significantly decreased as compared with healthy controls.

Similarity: Contains 1 C1q domain.

Similarity: Contains 1 collagen-like domain.

OMIM: 605441; gene+phenotype. [NCBI / EBI]

Products similar to ADIPOQ protein:

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Human Adiponectin ELISA, ELISA_Kits

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Mouse Adiponectin ELISA, ELISA_Kits

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SUGGESTED PROTEIN REAGENTS - GenWay offers reagents to facilitate your research with cell-based assays, Mass Spectrometry, standards, calibrators, Western blot, cell culture, antibiotics, microwell substrates, and more!

BACKGROUND REFERENCES for ADIPOQ PROTEIN:

Background references for antibody target are not specific to GenWay products

1. Joachim Fruebis, et al(2001) PNAS 98(4) 2005-2010
1. Das,K., et al(2001) Biochem. Biophys.Res.Commun. 280(4)1120-1129
1. Yamauchi T., et al(2001) Nature Medicine 7(8) 941-946
1. Berg AH, et al(2001) Nature Medicine 7(8) 947-952
1. Yamauchi. T., et al(2002) Nature Medicine 8(11) 1288-1295

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: ADIPOQ protein is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of ADIPOQ protein vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about ADIPOQ protein, all related information, articles, resources about ADIPOQ protein are being stored on our online database. Let us know if you have questions regarding this product.

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