Alphabetical Listing of Primary Antibodies:
f
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0-4
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5-9
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A
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B
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C
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D
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E
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F
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G
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H
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I
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J
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K
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L
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M
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N
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O
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P
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Q
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R
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S
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T
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U
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V
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W
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X
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Y
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Z
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Alphabetical Listing of Proteins:
f
|
0-4
|
5-9
|
A
|
B
|
C
|
D
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E
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F
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G
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H
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I
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J
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K
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L
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M
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N
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O
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P
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Q
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R
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S
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T
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U
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V
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W
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X
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Y
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Z
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Peptides
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Secondary Antibodies
|
ELISA Kits
Hif-1 alpha recombinant protein
Catalog Number: 10-002-38080
Buy HIF1A protein -
Size:
0.5 mg ($540.00)
0.1 mg ($270.00)
Related Product Names:
- HIF1A protein; HIF1A; MOP1; Hif-1 alpha
- Hif-1 alpha (530-826 aa) Human, Recombinant, E Coli; HIF-1 alpha; HIF1 alpha; ARNT-interacting protein; Member of PAS protein 1; Basic-helix-loop-helix-PAS protein MOP1; Hypoxia-inducible factor 1 alpha
- Gene Information -
Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact
sales@genwaybio.com
.
Gene Name:
HIF1A
Gene Name Synonym:
MOP1
Gi #:
N/A
NCBI Acc #:
NP_001521.1
Swiss Prot Acc #:
Q16665
Length (aa):
N/A
Mol. Weight (Da):
92670
Chrom Location:
N/A
Molecular Weight:
32.8 kDa (298 a.a). (The recombinant protein appeared as a 40 kDa band on SDS-PAGE)
Sequence:
MEFKLELVEK LFAEDTEAKN PFSTQDTDLD LEMLAPYIPM DDDFQLRSFD QLSPLESSSA SPESASPQST VTVFQQTQIQ EPTANATTTT ATTDELKTVT KDRMEDIKIL IASPSPTHIH KETTSATSSP YRDTQSRTAS PNRAGKGVIE QTEKSHPRSP NVLSVALSQR TTVPEEELNP KILALQNAQR KRKMEHDGSL FQAVGIGTLL QQPDDHAATT SLSWKRVKGC KSSEQNGMEQ KTIILIPSDL ACRLLGQSMD ESGLPQLTSY DCEVNAPIQG SRNLLQGEEL LRALDQVN
Concentration:
1mg/ml
Purity/Purification:
>= 95% by SDS PAGE
Format:
Liquid. In 20mM Tris-HCl pH7.5, 1mMDTT
Storage:
Store at -20C. Avoid freeze/thaw cycles.
Shipping:
Products may be shipped on ice pack or dry ice.
TESTING: (
secondary reagents
and
protocols
)
WB ( anti-HIF1A protein )
HIF1A protein image
SDS ( anti-HIF1A protein )
HIF1A protein image
HIF1A PROTEIN TARGET DESCRIPTION:
Synonym Names for HIF1A protein:
HIF1A; MOP1; Hif-1 alpha (530-826 aa) Human, Recombinant, E Coli; HIF-1 alpha; HIF1 alpha; ARNT-interacting protein; Member of PAS protein 1; Basic-helix-loop-helix-PAS protein MOP1; Hypoxia-inducible factor 1 alpha
Hypoxia-inducible factor-1 (HIF-1), identified as one of the transcription factors, has been found to play an essential role in oxygen homeostasis. HIF-1 is a heterodimer composed of HIF-1beta subunit and one of three subunits (Hif-1alpha, Hif-2 ( or Hif-3)). The activation of Hif-1 ( is closely associated with a variety of tumors and oncogenic pathways. Hif-1 ( consists of DNA binding domain (DBD domain), Dimerization domain and C-terminla regulatiory domains, including two transactivation domains (TAD), an oxygen-dependent degradation (ODD) domain, and inhibitory domains. Hif-1 ( (530-826 residues) contains two TAD and inhibitory domain. Recombinant Hif-1 ( (530-826 residues) was expressed in E.coli and purified by using conventional chromatography techniques.
Function:
Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP.
Subunit:
Interacts with the HIF1A beta/ARNT subunit; heterodimerization is required for DNA binding. Interacts with COPS5; the interaction increases the transcriptional activity of HIF1A through increased stability (By similarity). Interacts with CREBBP and EP300 (via TAZ-type 1 domains). Interacts with NCOA1, NCOA2, APEX and HSP90. Interacts (hydroxylated within the ODD domain) with VHLL (via beta domain); the interaction, leads to polyubiquitination and subsequent HIF1A proteasomal degradation. During hypoxia, sumoylated HIF1A also binds VHL; the interaction promotes the ubiquitination of HIF1A. Interacts with SENP1; the interaction desumoylates HIF1A resulting in stabilization and activation of transcription. Interacts (Via the ODD domain) with ARD1A; the interaction appears not to acetylate HIF1A nor have any affect on protein stability, during hypoxia. Interacts with RWDD3; the interaction enhances HIF1A sumoylation. Interacts with TSGA10 (By similarity).
Subcellular Location:
Cytoplasm. Nucleus. Note=Cytoplasmic in normoxia, nuclear translocation in response to hypoxia. Colocalizes with SUMO1 in the nucleus, under hypoxia.
Tissue Specificity:
Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors.
Induction:
Under reduced oxygen tension. Induced also by various receptor-mediated factors such as growth factors, cytokines, and circulatory factors such as PDGF, EGF FGF-2 FGF-2 IGF-2, TGF-1 beta, HGF, TNF alpha, IL-1 beta, angiotensin-2 and thrombin. However, this induction is less intense than that stimulated by hypoxia.
Domain:
Contains two independent C-terminal transactivation domains, NTAD and CTAD, which function synergistically. Their transcriptional activity is repressed by an intervening inhibitory domain (ID).
Ptm:
In normoxia, is hydroxylated on Pro-402 and Pro-564 in the oxygen-dependent degradation domain (ODD) by EGLN1/PHD1 and EGLN2/PHD2. EGLN3/PHD3 has also been shown to hydroxylate Pro-564. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization.
Ptm:
In normoxia, is hydroxylated on Asn-803 by HIF1AN, thus abrogating interaction with CREBBP and EP300 and preventing transcriptional activation. This hydroxylation is inhibited by the Cu/Zn-chelator, Clioquinol.
Ptm:
S-nitrosylation of Cys-800 may be responsible for increased recruitment of p300 coactivator necessary for transcriptional activity of HIF-1 complex.
Ptm:
Requires phosphorylation for DNA-binding.
Ptm:
Sumoylated; by SUMO1 under hypoxia. Sumoylation is enhanced through interaction with RWDD3. Desumoylation by SENP1 leads to increased HIF1A stability and transriptional activity (By similarity).
Ptm:
Ubiquitinated; in normoxia, following hydroxylation and interaction with VHL. Lys-532 appears to be the principal site of ubiquitination. Clioquinol, the Cu/Zn-chelator, inhibits ubiquitination through preventing hydroxylation at Asn-803.
Similarity:
Contains 1 basic helix-loop-helix (bHLH) domain.
Similarity:
Contains 1 PAC (PAS-associated C-terminal) domain.
Similarity:
Contains 2 PAS (PER-ARNT-SIM) domains.
OMIM:
603348; gene. [
NCBI
/
EBI
]
Pathways:
KEGG pathway: Renal cell carcinoma
05211
KEGG pathway: mTOR signaling pathway
04150
Products similar to HIF1A protein:
IgG
HIF1AN (hypoxia-inducible factor 1. alpha subunit inhibitor), IgG
HIF1AN (hypoxia-inducible factor 1. alpha subunit inhibitor), IgG
HIF1 alpha [mgc3], IgG
HIF1AN (hypoxia-inducible factor 1. alpha subunit inhibitor), IgG
HIF-1 Alpha Human, Protein
PRODUCT INTERACTIONS:
Hif-1 alpha
interacts
with
Anti-Dihydrotestosterone, IgG
(GenWay Catalog #: 10-002-38080)
.
Hif-1 alpha
interacts
with
Anti-Dihydrotestosterone, IgG
(GenWay Catalog #: 10-002-38080)
.
Hif-1 alpha
interacts
with
p300 [3G230], IgG
(GenWay Catalog #: 10-002-38080)
.
Hif-1 alpha
interacts
with
p300, IgG
(GenWay Catalog #: 10-002-38080)
.
Hif-1 alpha
interacts
with
p300, IgG
(GenWay Catalog #: 10-002-38080)
.
Hif-1 alpha
interacts
with
HIF1AN (hypoxia-inducible factor 1. alpha subunit inhibitor), IgG
(GenWay Catalog #: 10-002-38080)
.
Hif-1 alpha
interacts
with
HIF1AN (hypoxia-inducible factor 1. alpha subunit inhibitor), IgG
(GenWay Catalog #: 10-002-38080)
.
Hif-1 alpha
interacts
with
HIF1AN (hypoxia-inducible factor 1. alpha subunit inhibitor), IgG
(GenWay Catalog #: 10-002-38080)
.
Hif-1 alpha
interacts
with
MAX, IgG
(GenWay Catalog #: 10-002-38080)
.
Hif-1 alpha
interacts
with
MAX, IgG
(GenWay Catalog #: 10-002-38080)
.
Hif-1 alpha
interacts
with
Mad Max, IgG
(GenWay Catalog #: 10-002-38080)
.
Hif-1 alpha
interacts
with
PCAF (p300/CBP-associated factor), IgG
(GenWay Catalog #: 10-002-38080)
.
Hif-1 alpha
interacts
with
PCAF, IgG
(GenWay Catalog #: 10-002-38080)
.
SUGGESTED PROTEIN REAGENTS
- GenWay offers reagents to facilitate your research with cell-based assays, Mass Spectrometry, standards, calibrators, Western blot, cell culture, antibiotics, microwell substrates, and more!
BACKGROUND REFERENCES for HIF1A PROTEIN:
Background references for antibody target are not specific to GenWay products
1. Okuyama H. et al., (2006) J. Biol Chem. 281(22): 15554-63
1. Berra E. et al., (2006) EMBO.7(1): 41-5
Order Confirmation:
Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.
Precautions:
HIF1A protein is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.
Important Notes:
During shipment, small volumes of HIF1A protein vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.
Copyright:
This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about HIF1A protein, all related information, articles, resources about HIF1A protein are being stored on our online database. Let us know if you have questions regarding this product.
Disclaimer:
For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed.
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