RUVBL2 antibody, RUVBL2 (RuvB-like 2 (E. coli)) Antibody, Rabbit Polyclonal Antibody

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RUVBL2 (RuvB-like 2 (E. coli)) Antibody, Rabbit Polyclonal Antibody

Catalog Number: 18-003-42194

Buy RUVBL2 antibody -

Size:

Trial Size Available. Please expect delivery of trial size products to be 4 weeks.

Related Product Names:

- RUVBL2 antibody; RUVBL2; CGI-46, ECP51, TIP48, TIP49B; RUVBL2 (RuvB-like 2 (E. coli))
- EC 3.6.1.-; 48 kDa TATA box-binding protein-interacting protein; 48 kDa TBP-interacting protein; TIP49b; Repressing pontin 52; Reptin 52; 51 kDa erythrocyte cytosolic protein; ECP-51; TIP60-associated protein 54-beta; TAP54-beta; RuvB-like 2

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: RUVBL2	Gene Name Synonym: CGI-46, ECP51, TIP48, TIP49B
Gi #: 5730023	NCBI Acc #: NP_006657.1	Swiss Prot Acc #: Q9Y230
Length (aa): 463	Mol. Weight (Da): 51157	Chrom Location: N/A

Antigen Specificity: Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human RUVBL2.

ELISA Titre: 1:1562500

Note: Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity.

Source/Host: Rabbit

Purity/Purification: Rabbit Ig G is purified by peptide affinity chromatography method.

Clonality: Polyclonal

Crossreactivity: Human

Format: Suggested dilution at 0.5 microg/ml in 5% skim milk / PBS buffer. and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50.000 - 100.000 as second antibody.

Storage: Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 microl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage. store at -20C.

Stability: Note: Avoid repeat freeze-thaw cycles.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for RUVBL2 ANTIBODY:

IHC-P. WB: Tested

TESTING: (secondary reagents and protocols [IHC Protocol])

IHC: ( anti-RUVBL2 )

RUVBL2 antibody IHC image
RUVBL2

WB: ( anti-RUVBL2 )

RUVBL2 antibody WB image
RUVBL2

RUVBL2 ANTIBODY TARGET DESCRIPTION:

Synonym Names for RUVBL2 antibody: RUVBL2; CGI-46, ECP51, TIP48, TIP49B; EC 3.6.1.-; 48 kDa TATA box-binding protein-interacting protein; 48 kDa TBP-interacting protein; TIP49b; Repressing pontin 52; Reptin 52; 51 kDa erythrocyte cytosolic protein; ECP-51; TIP60-associated protein 54-beta; TAP54-beta; RuvB-like 2

RUVBL2 encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and

Function: Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL2 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2.

Subunit: Forms a multimeric complex with RUVBL1. Interacts with the transcriptional activation domain of MYC. Interacts With ATF2. Component of the RNA polymerase II holoenzyme complex. May also act to bridge the LEF1/TCF1-CTNNB1 complex and TBP. Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit HTATIP/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41 and VPS72/YL1. The NuA4 complex interacts with MYC and the adenovirus E1A protein. RUVBL2 interacts with EP400. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, TCFL1/YL1 and YEATS4/GAS41. Interacts with NPAT.

Subcellular Location: Nucleus matrix. Nucleus, nucleoplasm. Cytoplasm. Membrane. Note=Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol. Although it is also present in the cytoplasm and associated with the cell membranes.

Tissue Specificity: Ubiquitously expressed. Highly expressed in testis and thymus.

Domain: The C-terminal domain is required for association with ATF2.

Ptm: Phosphorylated upon DNA damage, probably by ATM or ATR.

Similarity: Belongs to the ruvB family.

Summary: This protein is the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities. This protein is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation.

RUVBL2 (RuvB-like 2 (E. coli)) reacts with human.

OMIM: 604788; gene. [NCBI / EBI]

Products similar to RUVBL2 antibody:

IgG

RUVBL2 (RuvB-like 2 (E. coli)), IgG

PRODUCT INTERACTIONS:

RUVBL2 (RuvB-like 2 (E. coli)) interacts with Deoxyribonuclease-2-alpha, IgG (GenWay Catalog #: 18-003-42194).

RUVBL2 (RuvB-like 2 (E. coli)) interacts with EXOSC10 (exosome component 10), IgG (GenWay Catalog #: 18-003-42194).

RUVBL2 (RuvB-like 2 (E. coli)) interacts with PM/Scl 100 Human, Protein (GenWay Catalog #: 18-003-42194).

RUVBL2 (RuvB-like 2 (E. coli)) interacts with Inhibitor of nuclear factor kappa-B kinase epsilon subunit, IgG (GenWay Catalog #: 18-003-42194).

RUVBL2 (RuvB-like 2 (E. coli)) interacts with IKK iota + IKK epsilon [72B587], IgG (GenWay Catalog #: 18-003-42194).

RUVBL2 (RuvB-like 2 (E. coli)) interacts with IKK iota + IKK epsilon, IgG (GenWay Catalog #: 18-003-42194).

RUVBL2 (RuvB-like 2 (E. coli)) interacts with Anti-IKKe pT501, IgG (GenWay Catalog #: 18-003-42194).

RUVBL2 (RuvB-like 2 (E. coli)) interacts with NDRG1 (N-myc downstream regulated gene 1), IgG (GenWay Catalog #: 18-003-42194).

RUVBL2 (RuvB-like 2 (E. coli)) interacts with Recombinant Human N-Myc Downstream Regulated 1, Protein (GenWay Catalog #: 18-003-42194).

RUVBL2 (RuvB-like 2 (E. coli)) interacts with Recombinant Human Non-Metastatic Cells 2, Native_Protein (GenWay Catalog #: 18-003-42194).

RUVBL2 (RuvB-like 2 (E. coli)) interacts with RGS20 (regulator of G-protein signalling 20), IgG (GenWay Catalog #: 18-003-42194).

RUVBL2 (RuvB-like 2 (E. coli)) interacts with RuvB-like 1 (E. coli) (RUVBL1), IgG (GenWay Catalog #: 18-003-42194).

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

BACKGROUND REFERENCES for RUVBL2 ANTIBODY:

Background references for antibody target are not specific to GenWay products

Bauer.A.. et al.. (2000) EMBO J. 19 (22). 6121-6130.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: RUVBL2 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of RUVBL2 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about RUVBL2 antibody, all related information, articles, resources about RUVBL2 antibody are being stored on our online database. Let us know if you have questions regarding this product.

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