Alphabetical Listing of Primary Antibodies:
f
|
0-4
|
5-9
|
A
|
B
|
C
|
D
|
E
|
F
|
G
|
H
|
I
|
J
|
K
|
L
|
M
|
N
|
O
|
P
|
Q
|
R
|
S
|
T
|
U
|
V
|
W
|
X
|
Y
|
Z
|
Alphabetical Listing of Proteins:
f
|
0-4
|
5-9
|
A
|
B
|
C
|
D
|
E
|
F
|
G
|
H
|
I
|
J
|
K
|
L
|
M
|
N
|
O
|
P
|
Q
|
R
|
S
|
T
|
U
|
V
|
W
|
X
|
Y
|
Z
|
Peptides
|
Secondary Antibodies
|
ELISA Kits
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa) Antibody, Rabbit Polyclonal Antibody
Catalog Number: 18-003-42223
Buy TAF1 antibody -
Size:
0.05 mg ($320.00)
Related Product Names:
- TAF1 antibody; TAF1; BA2R, CCG1, CCGS, NSCL2, OF, P250, TAF2A, TAFII250; TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
- EC 2.7.11.1; Transcription initiation factor TFIID 250 kDa subunit; TAF(II)250; TAFII-250; TAFII250; TBP-associated factor 250 kDa; p250; Cell cycle gene 1 protein; TBP-associated factor 1 isoform 2; Transcription initiation factor TFIID subunit 1
- Gene Information -
Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact
sales@genwaybio.com
.
Gene Name:
TAF1
Gene Name Synonym:
BA2R, CCG1, CCGS, NSCL2, OF, P250, TAF2A, TAFII250
Gi #:
20357588
NCBI Acc #:
NP_620278.1
Swiss Prot Acc #:
P21675
Length (aa):
1872
Mol. Weight (Da):
212677
Chrom Location:
N/A
Antigen Specificity:
Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human TAF1.
ELISA Titre:
1:62500
Category:
Transcription Factor Antibodies
Note:
Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity.
Source/Host:
Rabbit
Purity/Purification:
Rabbit Ig G is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Crossreactivity:
Human
Format:
Suggested dilution at 0.5 microg/ml in 5% skim milk / PBS buffer. and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50.000 - 100.000 as second antibody.
Storage:
Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 microl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage. store at -20C.
Stability:
Note: Avoid repeat freeze-thaw cycles.
Shipping:
Products may be shipped on ice pack or dry ice.
APPLICATIONS for TAF1 ANTIBODY:
IHC-P. WB: Tested
TESTING: (
secondary reagents
and
protocols
[IHC Protocol]
)
IHC:
( anti-TAF1 )
TAF1 antibody IHC image
WB:
( anti-TAF1 )
TAF1 antibody WB image
TAF1 ANTIBODY TARGET DESCRIPTION:
Synonym Names for TAF1 antibody:
TAF1; BA2R, CCG1, CCGS, NSCL2, OF, P250, TAF2A, TAFII250; EC 2.7.11.1; Transcription initiation factor TFIID 250 kDa subunit; TAF(II)250; TAFII-250; TAFII250; TBP-associated factor 250 kDa; p250; Cell cycle gene 1 protein; TBP-associated factor 1 isoform 2; Transcription initiation factor TFIID subunit 1
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID. which binds to the core promoter to position the polymerase prope
Function:
Largest component and core scaffold of the TFIID basal transcription factor complex. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity. Essential for progression of the G1 phase of the cell cycle.
Catalytic Activity:
ATP + a protein = ADP + a phosphoprotein.
Cofactor:
Magnesium.
Enzyme Regulation:
Autophosphorylates on Ser residues. Inhibited by retinoblastoma tumor suppressor protein, RB1.
Subunit:
TAF1 is the largest component of transcription factor TFIID that is composed of TBP and a variety of TBP-associated factors. TAF1, when part of the TFIID complex, interacts with C-terminus of TP53. RB1 interacts with the N-terminal domain of TAF1. Interacts with ASF1A and ASF1B.
Subcellular Location:
Nucleus.
Ptm:
Phosphorylated by casein kinase II in vitro.
Disease:
Defects in TAF1 are the cause of X-linked torsion dystonia type 3 (DYT3) [MIM:314250]; also called X-linked dystonia-parkinsonism (XDP). DYT3 is a movement disorder characterized by severe progressive torsion dystonia followed by parkinsonism. The disorder is caused by an SVA (short interspersed nuclear element variable number of tandem repeats and Alu composite) retrotransposon insertion in intron 32 of the TAF1 gene.
Similarity:
Belongs to the TAF1 family.
Similarity:
Contains 2 bromo domains.
Similarity:
Contains 1 HMG box DNA-binding domain.
Similarity:
Contains 2 protein kinase domains.
Summary:
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This protein is the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. Two transcripts encoding different isoforms have been identified for this gene.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa) reacts with human.
OMIM:
313650; gene. [
NCBI
/
EBI
]
314250; phenotype. [
NCBI
/
EBI
]
Products similar to TAF1 antibody:
IgG
TAF15 (TAF15 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 68kDa), IgG
TAF1A (TATA box binding protein (TBP)-associated factor. RNA polymerase I. A. 48kDa), IgG
TBP Associated Factor 1 [0.T.145], IgG
TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa, IgG
BTAF1, IgG
PRODUCT INTERACTIONS:
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
GTF2F1 (general transcription factor IIF. polypeptide 1. 74kDa), IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
retinoblastoma 1 (including osteosarcoma) (RB1), IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
RETINOBLASTOMA, IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
RB cleaved [172C1094], IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
RB1 phospho T821, IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
RB1 phospho S807, IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
RB1 phospho T826, IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
RB1 phospho S780, IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
RB1 phospho S612, IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
RB1 [Rb1], IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
RB1 [4G2], IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
RB1 [4H6], IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
RB1 [7F12], IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
RB1 [1G5], IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
RB1 [1E5], IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
RB1 [1D1], IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
RB1 [3C8], IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
RB1 [1F8], IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
Rb (Phospho-Ser795), IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
Rb (Phospho-Ser807), IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
Rb (Phospho-Ser780), IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
Rb (Ab-795), IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
Rb (Ab-807), IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
Rb (Ab-780), IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
Rb (Phospho-Ser795) Antibody Blocking Peptide, Peptide
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
Rb (Phospho-Ser807) Antibody Blocking Peptide, Peptide
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
Rb (Phospho-Ser780) Antibody Blocking Peptide, Peptide
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
RB1 (retinoblastoma 1 (including osteosarcoma)), IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
Rb Protein, IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
RB1 Human, Protein
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
RB1 Human, Protein
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
RETINOBLASTOMA, IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
RETINOBLASTOMA, IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
RETINOBLASTOMA, IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
RB1 phospho S249, IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
RB1 phospho S811, IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
RB1 phospho T356, IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
RB1 [1F8] (ready-to-use), IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
RB1 phospho S608 [51B7], IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
RB1 phospho S807, S811, IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
RB1 (ready-to-use), IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
TATA box binding protein, IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
TATA binding protein TBP [1TB18], IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
TATA binding protein TBP [1TBP18], IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
TBP (TATA box binding protein), IgG
(GenWay Catalog #: 18-003-42223)
.
TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa)
interacts
with
TATA-Binding Protein, IgG
(GenWay Catalog #: 18-003-42223)
.
SUGGESTED ANITBODY REAGENTS
- GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.
BACKGROUND REFERENCES for TAF1 ANTIBODY:
Background references for antibody target are not specific to GenWay products
Lively.T.N.. et al.. (2004) J. Biol. Chem. 279 (25). 26257-26265.
Order Confirmation:
Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.
Precautions:
TAF1 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.
Important Notes:
During shipment, small volumes of TAF1 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.
Copyright:
This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about TAF1 antibody, all related information, articles, resources about TAF1 antibody are being stored on our online database. Let us know if you have questions regarding this product.
Disclaimer:
For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed.
GenWay is a Protein and Antibody Solutions Provider. Customer Satisfaction is Our Top Priority.
Thank you for visiting GenWay Biotech's Datasheet. GenWay is the world's leader in chicken, IgY antibody production, producing over 900 quality polyclonal IgY antibodies to date. From our unique recombinant protein production, utilizing a variety of protein expression systems, GenWay's polyclonal IgY antibodies incorporate more epitopes, increasing the success rate for ELISA, western blot, immunohistochemistry (IHC), immunocytochemistry (ICC), and many more immunoassays.
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Matrix protein 2
MB Human
MCM2 (minichromosome maintenance complex...
MCM4 (minichromosome maintenance complex...
MCM4 (minichromosome maintenance complex...
MCM4 (minichromosome maintenance complex...
MCM5 (minichromosome maintenance complex...
MCM6 (minichromosome maintenance complex...
MCM7 (minichromosome maintenance complex...
MCP-1 Human
MCP-1 Mouse
MCP-1 Rat
MCP-1 receptor
MCP-1 receptor
MCP-3 Human
MCP-3 Mouse
MDH
Methylated-DNA--protein-cysteine methylt...
MIA Human
MICA Human
MIF
MIF (clone 4E4)
MIF Human His-N
MIG Human
MIG Mouse
MIP-1a Human
MIP-1a Mouse
MIP-1a Rat
MIP-1b Human
MITF (microphthalmia-associated transcri...
Mitogen-activated protein kinase 1
MMP-3 Human
MMP-7 Human
MMP2
MMP9
MTF1 (metal-regulatory transcription fac...
MYCBP (c-myc binding protein)
Myeloid differentiation primary response...
Myeloid differentiation primary response...
NFkappaB essential modulator
NFkappaB essential modulator
NRG1 Human
OPG Human, His
Orphan G Protein-Coupled Receptor CAD696...
Orphan G Protein-Coupled Receptor CAD696...
P-Sel Human
p21 Human
P27 kip1 protein
P40
p53 Human
p53M Human
Parathyroid Hormone
PCK1 (phosphoenolpyruvate carboxykinase ...
PCNA (proliferating cell nuclear antigen...
PCNA Human
PDI Human
Peflin
Peptidyl-prolyl cis-trans isomerase NIMA...
Peripheral-type benzodiazepine receptor
PEX Human
PfHSP70
PHB (prohibitin)
Pin1 (clone 3G8)
PIN1 Human
PIXY321 Human
PKC-a Human
PL-7 Human
PLGF-1 Human
PLGF-2 Human
PRL-R Bovine
PRL-R Ovine
Pro-epidermal growth factor
Probable G-protein coupled receptor 1
Probable G-protein coupled receptor 1
Probable G-protein coupled receptor 1
Prolactin
Prolactin Human
Prolactin Ovine
Prostate-specific antigen
Protein p65
PTEN Human
PTH 1-34 Human
PTH Human 1-84
Putative G-protein coupled receptor
Putative G-protein coupled receptor
Putative transmembrane receptor
Putative transmembrane receptor
Putative transmembrane receptor
RAC-alpha serine/threonine-protein kinas...
Rantes Human
RAP Rat
Ras-related C3 botulinum toxin substrate...
RB1 (retinoblastoma 1 (including osteosa...
Regulatory-associated protein of mTOR
Regulatory-associated protein of mTOR
Regulatory-associated protein of mTOR
RELA
Replication factor C subunit 5
Repressor of tet operon
RTSbeta
SALF (Stonin 1)
SARS Core 1-49
SARS Core 1-49, 192-220
SARS Core 340-390
SCD (stearoyl-CoA desaturase (delta-9-de...
SCF (Stem Cell Factor)
SCF Human
SCF Human, Sf9
SCF Mouse
SCF Rat
SDF-1a Human
SDF-1a Mouse
SDF-1b Human
SDF-1b Mouse
Serum amyloid P-component
Signal transducer and activator of trans...
SNAP-25 C.elegans
SOD Human
SPA
SPI1 (spleen focus forming virus (SFFV) ...
SPP1
Staphylokinase
STAT1 Human
STAT3 Human
STAT4 (signal transducer and activator o...
Substance-P receptor
Substance-P receptor
T-cell-specific surface glycoprotein CD2...
T.gondii p30
TAF1 (TAF1 RNA polymerase II. TATA box b...
TaqDNA
TATA-binding protein-associated phosphop...
TBP (TATA box binding protein)
Thaumatin-2
TIRP
TIRP
TLR2 (clone 1F10)
TLR7 (clone 4F4)
TNF-b Human
TNFRI Human
Toll-like receptor 2
Toll-like receptor 4
Toll-like receptor 4
Toll-like receptor 7
TOP1 Human
TP53 (tumor protein p53 (Li-Fraumeni syn...
tPA Human
TPO Human
TPO Human
TPO Mouse
TRX Yeast
Trypsin Bovine
Tumor necrosis factor receptor superfami...
Ubiquitin Human
VEGF (121) Human, Sf9
VEGF Human, Sf9
Vim Human
YY1 (YY1 transcription factor)
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Xenopus
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Xreactivity:
Amphibian
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Drosophila
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Human
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