ENO1 antibody, ENO1 (enolase 1. (alpha)) Antibody, Rabbit Polyclonal Antibody

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Peptides \| Secondary Antibodies \| ELISA Kits

ENO1 (enolase 1. (alpha)) Antibody, Rabbit Polyclonal Antibody

Catalog Number: 18-003-42514

Buy ENO1 antibody -

Size:

Trial Size Available. Please expect delivery of trial size products to be 4 weeks.

Related Product Names:

- ENO1 antibody; ENO1; ENO1L1, MBP-1, MPB1, NNE, PPH; ENO1 (enolase 1. (alpha))
- EC 4.2.1.11; Enolase 1; Enolase

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: ENO1	Gene Name Synonym: ENO1L1, MBP-1, MPB1, NNE, PPH
Gi #: 4503571	NCBI Acc #: NP_001419.1	Swiss Prot Acc #: Q53HR3
Length (aa): 434	Mol. Weight (Da): 47141	Chrom Location: N/A

Antigen Specificity: Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human ENO1.

ELISA Titre: 1:62500

Note: Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity.

Protocols from Publications
Citation: 1: Lü X, de la Peña L, Barker C, Camphausen K, Tofilon PJ. Radiation-induced changes in gene expression involve recruitment of existing messenger RNAs to and away from polysomes. Cancer Res. 2006 Jan 15;66(2):1052-61. PubMed PMID: 16424041.

Experiment Name: Immunoblot analysis for Enolase 1 in U87 cells after irradiation (7 Gy).

Experiment Background: To determine whether the radiation-induced change in a gene's translational activity (as indicated by polysome binding) corresponds with a change in its protein product (here Enolase 1),

Experiment Steps: 1. Rinse cells with ice-cold PBS.
2. Scrap cells into lysis buffer containing 50 mmol/L Tris (pH 7.4), 150 mmol/L NaCl, 1 mmol/L EDTA, 1% NP40, supplemented with protease inhibitor cocktail and phosphatase inhibitors I and II.
3. Separate insoluble material after centrifugation at 14,000 rpm.
4. Subject proteins to SDS-PAGE using NuPage 4% to 12% gels and NuPage MES or MOPS buffers.
5. After electrophoresis, electroblot gels onto polyvinylidene difluoride membranes.
6. At room temperature block the nonspecific sites on the membranes for 30 minutes with 5% nonfat milk in TBS supplemented with 0.2% Tween 20 (TBS-T).
7. Block membranes in blocking solution overnight at 4C with enolase 1.
8. Wash membranes thrice in TBS-T.
9. Incubate with the appropriate horseradish peroxidase-conjugated secondary antibody at a 1:2,000 dilution in blocking solution for 1 hour at room temperature.
10. Again wash membranes thrice in TBS-T.
11. Develop membranes with enhanced chemiluminescence western blotting detection reagents.
12. Visualize Membranes with a Typhoon scanner.

Source/Host: Rabbit

Purity/Purification: Rabbit IgG purified by protein A chromatography.

Clonality: Polyclonal

Crossreactivity: Human

Format: Suggested dilution at 1.25 microg/ml in 5% skim milk / PBS buffer. and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50.000 - 100.000 as second antibody.

Storage: Antibody is lyophilized from PBS buffer with 2% sucrose. Add 100 microl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage. store at -20C.

Stability: Note: Avoid repeat freeze-thaw cycles.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for ENO1 ANTIBODY:

WB: Tested

TESTING: (secondary reagents and protocols [IHC Protocol])

WB: ( anti-ENO1 )

ENO1 antibody WB image
ENO1

ENO1 ANTIBODY TARGET DESCRIPTION:

Synonym Names for ENO1 antibody: ENO1; ENO1L1, MBP-1, MPB1, NNE, PPH; EC 4.2.1.11; Enolase 1; Enolase

ENO1 is one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.

Catalytic Activity: 2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactor: Magnesium. Required for catalysis and for stabilizing the dimer (By similarity).

Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.

Similarity: Belongs to the enolase family.

Summary: This protein is one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.

ENO1 (enolase 1. (alpha)) reacts with human.

OMIM: NCBI [172430]

Products similar to ENO1 antibody:

IgG

NON-NEURONAL ENOLASE, IgG

ENO1 (enolase 1. (alpha)), IgG

Non-Neuronal Enolase (NNE), Native_Protein

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

BACKGROUND REFERENCES for ENO1 ANTIBODY:

Background references for antibody target are not specific to GenWay products

Ghosh.A.K.. et al.. (2005) J. Biol. Chem. 280 (14). 14325-14330.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: ENO1 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of ENO1 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about ENO1 antibody, all related information, articles, resources about ENO1 antibody are being stored on our online database. Let us know if you have questions regarding this product.

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