SMAD2 antibody, SMAD2 (SMAD family member 2) Antibody, Rabbit Polyclonal Antibody

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Peptides \| Secondary Antibodies \| ELISA Kits

SMAD2 (SMAD family member 2) Antibody, Rabbit Polyclonal Antibody

Catalog Number: 18-003-43345

Buy SMAD2 antibody -

Size:

Trial Size Available. Please expect delivery of trial size products to be 4 weeks.

Related Product Names:

- SMAD2 antibody; SMAD2; JV18, JV18-1, MADH2, MADR2, MGC22139, MGC34440, hMAD-2, hSMAD2; SMAD2 (SMAD family member 2)
- SMAD 2; Mothers against DPP homolog 2; Mad-related protein 2; hMAD-2; JV18-1; hSMAD2; Sma- and Mad-related protein 2; Mothers against decapentaplegic homolog 2

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: SMAD2	Gene Name Synonym: JV18, JV18-1, MADH2, MADR2, MGC22139, MGC34440, hMAD-2, hSMAD2
Gi #: 5174511	NCBI Acc #: NP_005892.1	Swiss Prot Acc #: Q15796
Length (aa): 467	Mol. Weight (Da): 52306	Chrom Location: N/A

Antigen Specificity: Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human SMAD2.

ELISA Titre: 1:1562500

Note: Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity.

Source/Host: Rabbit

Purity/Purification: Rabbit IgG purified by protein A chromatography.

Clonality: Polyclonal

Crossreactivity: Human

Format: Suggested dilution at 2.5 microg/ml in 5% skim milk / PBS buffer. and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50.000 - 100.000 as second antibody.

Storage: Antibody is lyophilized from PBS buffer with 2% sucrose. Add 100 microl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage. store at -20C.

Stability: Note: Avoid repeat freeze-thaw cycles.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for SMAD2 ANTIBODY:

WB: Tested

TESTING: (secondary reagents and protocols [IHC Protocol])

WB: ( anti-SMAD2 )

SMAD2 antibody WB image
SMAD2

SMAD2 ANTIBODY TARGET DESCRIPTION:

Synonym Names for SMAD2 antibody: SMAD2; JV18, JV18-1, MADH2, MADR2, MGC22139, MGC34440, hMAD-2, hSMAD2; SMAD 2; Mothers against DPP homolog 2; Mad-related protein 2; hMAD-2; JV18-1; hSMAD2; Sma- and Mad-related protein 2; Mothers against decapentaplegic homolog 2

SMAD2 belongs to the SMAD. a family of proteins similar to the gene products of the Drosophila gene `mothers against decapentaplegic` (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multi

Function: Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.

Subunit: Found in a complex with SMAD3 and TRIM33 upon addition of TGF-beta. Interacts with SMAD3 and TRIM33. Interacts with SARA (SMAD anchor for receptor activation); may form trimers with the SMAD4 co-SMAD. Interacts with FOXH1, homeobox protein TGIF, PEBP2-alpha subunit, CREB-binding protein (CBP), p300, SKI, SNON and SMURF2. Interacts with AIP1 and HGS. Interacts with NEDD4L in response to TGF-beta (By similarity).

Subcellular Location: Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.

Tissue Specificity: Expressed at high levels in skeletal muscle, heart and placenta.

Ptm: Phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2. Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin.

Ptm: In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation (By similarity).

Disease: Defects in SMAD2 are found in sporadic cases of colorectal carcinoma.

Similarity: Belongs to the dwarfin/SMAD family.

Similarity: Contains 1 MH1 (MAD homology 1) domain.

Similarity: Contains 1 MH2 (MAD homology 2) domain.

Summary: The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed.

SMAD2 (SMAD family member 2) reacts with human.

OMIM: 601366; gene. [NCBI / EBI]

Products similar to SMAD2 antibody:

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SMAD2 / MADH2, IgG

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

BACKGROUND REFERENCES for SMAD2 ANTIBODY:

Background references for antibody target are not specific to GenWay products

Zheng.X.. (2006) EMBO J. 25 (3). 615-627.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: SMAD2 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of SMAD2 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about SMAD2 antibody, all related information, articles, resources about SMAD2 antibody are being stored on our online database. Let us know if you have questions regarding this product.

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