SFPQ antibody, SFPQ (splicing factor proline/glutamine-rich) Antibody, Rabbit Polyclonal Antibody

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Peptides \| Secondary Antibodies \| ELISA Kits

SFPQ (splicing factor proline/glutamine-rich) Antibody, Rabbit Polyclonal Antibody

Catalog Number: 18-003-43605

Buy SFPQ antibody -

Size:

Trial Size Available. Please expect delivery of trial size products to be 4 weeks.

Related Product Names:

- SFPQ antibody; SFPQ; PSF; SFPQ (splicing factor proline/glutamine-rich)
- Polypyrimidine tract-binding protein-associated-splicing factor; PTB-associated-splicing factor; PSF; DNA-binding p52/p100 complex, 100 kDa subunit; 100 kDa DNA-pairing protein; hPOMp100; Splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated); Splicing factor, proline- and glutamine-rich

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: SFPQ	Gene Name Synonym: PSF
Gi #: 4826998	NCBI Acc #: NP_005057.1	Swiss Prot Acc #: P23246
Length (aa): 707	Mol. Weight (Da): 76149	Chrom Location: N/A

Antigen Specificity: Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human SFPQ.

Western Blot: Suggested dilution at 5.0 microg/ml in 5% skim milk / PBS buffer. and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50.000 - 100.000 as second antibody.

ELISA Titre: 1:1562500

Category: RNA Binding Proteins

Note: Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity.

Source/Host: Rabbit

Purity/Purification: Rabbit IgG purified by protein A chromatography.

Clonality: Polyclonal

Crossreactivity: Human, Mouse, Rat

Storage: Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 ul of distilled water. Final antibody concentration is 2 mg/ml. For longer periods of storage. store at -20C.

Stability: Note: Avoid repeat freeze-thaw cycles.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for SFPQ ANTIBODY:

IHC-P. WB: Tested

TESTING: (secondary reagents and protocols [IHC Protocol])

IHC: ( anti-SFPQ )

SFPQ antibody IHC image
SFPQ

WB: ( anti-SFPQ )

SFPQ antibody WB image
SFPQ

SFPQ ANTIBODY TARGET DESCRIPTION:

Synonym Names for SFPQ antibody: SFPQ; PSF; Polypyrimidine tract-binding protein-associated-splicing factor; PTB-associated-splicing factor; PSF; DNA-binding p52/p100 complex, 100 kDa subunit; 100 kDa DNA-pairing protein; hPOMp100; Splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated); Splicing factor, proline- and glutamine-rich

SFPQ is DNA- and RNA binding protein. It is essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as an heteromer with NONO. It binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. It interacts with U5 snRNA. It may be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. SFPQ may be involved in homologous DNA pairing. SFPQ is involved in transcriptional regulation. It binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity.

Function: DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as an heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing; in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1; in vitro, stimulates dissociation of TOP1 from DNA after cleavage and enhances its jumping between separate DNA helices. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends; in vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. SFPQ is involved in transcriptional regulation. Transcriptional repression is probably mediated by an interaction of SFPQ with SIN3A and subsequent recruitment of histone deacetylases (HDACs). The SFPQ-NONO/SF-1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. SFPQ isoform Long binds to the DNA binding domains (DBD) of nuclear hormone receptors, like RXRA and probably THRA, and acts as transcriptional corepressor in absence of hormone ligands. Binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity.

Subunit: Interacts with PSPC1 (By similarity). Monomer and component of the SFPQ-NONO complex, which is probably a heterotetramer of two 52 kDa (NONO) and two 100 kDa (SFPQ) subunits. SFPQ is a component of spliceosome and U5.4/6 snRNP complexes. Interacts with SNRPA/U1A. Component of a snRNP-free complex with SNRPA/U1A. Part of complex consisting of SFPQ, NONO and MATR3. Interacts with polypyrimidine tract-binding protein 1/PTB. Part of a complex consisting of SFPQ, NONO and NR5A1/SF-1. Interacts with RXRA, probably THRA, and SIN3A. Interacts with TOP1. Part of a complex consisting of SFPQ, NONO and TOP1. Interacts with SNRP70 in apoptotic cells (By similarity).

Subcellular Location: Nucleus matrix. Note=Predominantly in nuclear matrix.

Ptm: The N-terminus is blocked.

Ptm: Phosphorylated on multiple serine and threonine residues during apoptosis. In vitro phosphorylated by PKC. Phosphorylation stimulates binding to DNA and D-loop formation, but inhibits binding to RNA.

Ptm: Arg-7, Arg-9, Arg-19 and Arg-25 are dimethylated, probably to asymmetric dimethylarginine.

Disease: A chromosomal aberration involving SFPQ may be a cause of papillary renal cell carcinoma (PRCC). Translocation t(X;1)(p11.2;p34) with TFE3.

Similarity: Contains 2 RRM (RNA recognition motif) domains.

SFPQ (splicing factor proline/glutamine-rich) reacts with human, mouse, rat.

OMIM: 605199; gene. [NCBI / EBI]

Products similar to SFPQ antibody:

IgG

GINS1 (GINS complex subunit 1 (Psf1 homolog)), IgG

CPSF3 (cleavage and polyadenylation specific factor 3. 73kDa), IgG

IGFBP7, IgG

cleavage and polyadenylation specific factor 4, 30kDa (CPSF4), mRNA, IgG

SFPQ [B92], IgG

Protein

DNA replication complex GINS protein PSF2 variant, Protein

PRODUCT INTERACTIONS:

SFPQ (splicing factor proline/glutamine-rich) interacts with NONO (non-POU domain containing. octamer-binding), IgG (GenWay Catalog #: 18-003-43605).

SFPQ (splicing factor proline/glutamine-rich) interacts with PTBP1 (polypyrimidine tract binding protein 1), IgG (GenWay Catalog #: 18-003-43605).

SFPQ (splicing factor proline/glutamine-rich) interacts with SNRPA (small nuclear ribonucleoprotein polypeptide A), IgG (GenWay Catalog #: 18-003-43605).

SFPQ (splicing factor proline/glutamine-rich) interacts with U1 snRNP A/B, IgG (GenWay Catalog #: 18-003-43605).

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

BACKGROUND REFERENCES for SFPQ ANTIBODY:

Background references for antibody target are not specific to GenWay products

Rosonina.E.. (2005) Mol. Cell. Biol. 25 (15). 6734-6746.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: SFPQ antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of SFPQ antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about SFPQ antibody, all related information, articles, resources about SFPQ antibody are being stored on our online database. Let us know if you have questions regarding this product.

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