Alphabetical Listing of Primary Antibodies:
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0-4
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5-9
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A
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B
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C
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D
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E
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F
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G
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H
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I
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J
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K
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L
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M
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N
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O
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P
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Q
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R
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S
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T
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U
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V
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W
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X
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Y
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Alphabetical Listing of Proteins:
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0-4
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5-9
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A
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B
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C
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D
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E
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F
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G
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H
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I
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J
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K
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L
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M
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N
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O
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P
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Q
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R
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S
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T
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U
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V
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W
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X
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Y
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Peptides
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Secondary Antibodies
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ELISA Kits
NONO (non-POU domain containing. octamer-binding) Antibody, Rabbit Polyclonal Antibody
Catalog Number: 18-003-43630
Buy NONO antibody -
Size:
0.1 mg ($255.00)
0.01 mg ($90.00)
Trial Size Available. Please expect delivery of trial size products to be 4 weeks.
Related Product Names:
- NONO antibody; NONO; NMT55, NRB54, P54, P54NRB; NONO (non-POU domain containing. octamer-binding)
- NonO protein; 54 kDa nuclear RNA- and DNA-binding protein; p54(nrb); p54nrb; 55 kDa nuclear protein; NMT55; DNA-binding p52/p100 complex, 52 kDa subunit; Non-POU domain containing, octamer-binding; Non-POU domain-containing octamer-binding protein
- Gene Information -
Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact
sales@genwaybio.com
.
Gene Name:
NONO
Gene Name Synonym:
NMT55, NRB54, P54, P54NRB
Gi #:
34932414
NCBI Acc #:
NP_031389.3
Swiss Prot Acc #:
Q15233
Length (aa):
471
Mol. Weight (Da):
54232
Chrom Location:
N/A
Antigen Specificity:
Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human NONO.
ELISA Titre:
1:62500
Category:
RNA Binding Proteins
Note:
Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity.
Source/Host:
Rabbit
Purity/Purification:
Rabbit IgG purified by protein A chromatography.
Clonality:
Polyclonal
Crossreactivity:
Human, Dog
Format:
Suggested dilution at 1.25 microg/ml in 5% skim milk / PBS buffer. and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50.000 - 100.000 as second antibody.
Storage:
Antibody is lyophilized from PBS buffer with 2% sucrose. Add 100 microl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage. store at -20C.
Stability:
Note: Avoid repeat freeze-thaw cycles.
Shipping:
Products may be shipped on ice pack or dry ice.
APPLICATIONS for NONO ANTIBODY:
IHC-P. WB: Tested
TESTING: (
secondary reagents
and
protocols
[IHC Protocol]
)
IHC:
( anti-NONO )
NONO antibody IHC image
WB:
( anti-NONO )
NONO antibody WB image
NONO ANTIBODY TARGET DESCRIPTION:
Synonym Names for NONO antibody:
NONO; NMT55, NRB54, P54, P54NRB; NonO protein; 54 kDa nuclear RNA- and DNA-binding protein; p54(nrb); p54nrb; 55 kDa nuclear protein; NMT55; DNA-binding p52/p100 complex, 52 kDa subunit; Non-POU domain containing, octamer-binding; Non-POU domain-containing octamer-binding protein
NONO is DNA- and RNA binding protein. involved in several nuclear processes. It binds the conventional octamer sequence in double stranded DNA. It also binds single-stranded DNA and RNA at a site independent of the duplex site. It is involved in pre-mRNA
Function:
DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site (By similarity). Involved in pre-mRNA splicing, probably as an heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. Nono is involved in transcriptional regulation. The SFPQ-NONO-NR5A1/SF-1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription (By similarity).
Subunit:
Monomer and component of the SFPQ-NONO complex, which is probably a heterotetramer of two 52 kDa (NONO) and two 100 kDa (SFPQ) subunits. NONO is a component of spliceosome and U5.4/6 snRNP complexes. Interacts with PSPC1 and SNRPA/U1A. Part of complex consisting of SFPQ, NONO and MATR3. Part of a complex consisting of SFPQ, NONO and NR5A1/SF-1. Part of a complex consisting of SFPQ, NONO and TOP1. Interacts with SPI1 (By similarity).
Subcellular Location:
Nucleus.
Tissue Specificity:
Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Also found in a number of breast tumor cell lines.
Ptm:
The N-terminus is blocked.
Disease:
A chromosomal aberration involving NONO may be a cause of papillary renal cell carcinoma (PRCC). Translocation t(X;X)(p11.2;q13.1) with TFE3.
Similarity:
Contains 2 RRM (RNA recognition motif) domains.
NONO (non-POU domain containing. octamer-binding) reacts with human, dog.
OMIM:
300084; gene. [
NCBI
/
EBI
]
Products similar to NONO antibody:
IgG
NONO (non-POU domain containing. octamer-binding), IgG
NONO / p54NRB, IgG
PRODUCT INTERACTIONS:
SUGGESTED ANITBODY REAGENTS
- GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.
BACKGROUND REFERENCES for NONO ANTIBODY:
Background references for antibody target are not specific to GenWay products
Kimura.K.. (2006) Genome Res. 16 (1). 55-65.
Order Confirmation:
Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.
Precautions:
NONO antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.
Important Notes:
During shipment, small volumes of NONO antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.
Copyright:
This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about NONO antibody, all related information, articles, resources about NONO antibody are being stored on our online database. Let us know if you have questions regarding this product.
Disclaimer:
For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed.
GenWay is a Protein and Antibody Solutions Provider. Customer Satisfaction is Our Top Priority.
Thank you for visiting GenWay Biotech's Datasheet. GenWay is the world's leader in chicken, IgY antibody production, producing over 900 quality polyclonal IgY antibodies to date. From our unique recombinant protein production, utilizing a variety of protein expression systems, GenWay's polyclonal IgY antibodies incorporate more epitopes, increasing the success rate for ELISA, western blot, immunohistochemistry (IHC), immunocytochemistry (ICC), and many more immunoassays.
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