PCAF antibody, PCAF (p300/CBP-associated factor) Antibody, Rabbit Polyclonal Antibody

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Peptides \| Secondary Antibodies \| ELISA Kits

PCAF (p300/CBP-associated factor) Antibody, Rabbit Polyclonal Antibody

Catalog Number: 18-003-43822

Buy PCAF antibody -

Size:

Related Product Names:

- PCAF antibody; PCAF; CAF, GCN5, GCN5L, GCN5L1, P/CAF; PCAF (p300/CBP-associated factor)
- EC 2.3.1.48; P300/CBP-associated factor; P/CAF; Histone acetylase PCAF; P300/CBP-associated factor; Histone acetyltransferase PCAF

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: PCAF	Gene Name Synonym: CAF, GCN5, GCN5L, GCN5L1, P/CAF
Gi #: 40805843	NCBI Acc #: NP_003875.3	Swiss Prot Acc #: Q92831
Length (aa): 832	Mol. Weight (Da): 93013	Chrom Location: N/A

Antigen Specificity: Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human PCAF.

ELISA Titre: 1:62500

Note: Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity.

Source/Host: Rabbit

Purity/Purification: Rabbit IgG purified by protein A chromatography.

Clonality: Polyclonal

Crossreactivity: Human, Mouse, Dog

Format: Suggested dilution at 2.5 microg/ml in 5% skim milk / PBS buffer. and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50.000 - 100.000 as second antibody.

Storage: Antibody is lyophilized from PBS buffer with 2% sucrose. Add 100 microl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage. store at -20C.

Stability: Note: Avoid repeat freeze-thaw cycles.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for PCAF ANTIBODY:

WB: Tested

TESTING: (secondary reagents and protocols [IHC Protocol])

WB: ( anti-PCAF )

PCAF antibody WB image
PCAF

PCAF ANTIBODY TARGET DESCRIPTION:

Synonym Names for PCAF antibody: PCAF; CAF, GCN5, GCN5L, GCN5L1, P/CAF; EC 2.3.1.48; P300/CBP-associated factor; P/CAF; Histone acetylase PCAF; P300/CBP-associated factor; Histone acetyltransferase PCAF

CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation. including c-jun and the adenoviral oncoprotein E1A. PCAF associates with p300/CBP. It has in vitro and in vivo binding acti

Function: Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes.

Catalytic Activity: Acetyl-CoA + histone = CoA + acetylhistone.

Subunit: Interacts with SIRT1 (By similarity). Interacts with EP300 and CREBBP. Interacts with NCOA1 and NCOA3. Binds to HTLV-1 Tax. Interacts with and acetylates HIV-1 Tat.

Subcellular Location: Nucleus (By similarity).

Tissue Specificity: Ubiquitously expressed but most abundant in heart and skeletal muscle.

Domain: The bromodomain mediates binding to HIV-1 Tat.

Similarity: Belongs to the GCN5 family.

Similarity: Contains 1 bromo domain.

Similarity: Contains 1 N-acetyltransferase domain.

Summary: CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation.

PCAF (p300/CBP-associated factor) reacts with human, mouse, dog.

OMIM: 602303; gene. [NCBI / EBI]

Products similar to PCAF antibody:

IgG

PCAF, IgG

PRODUCT INTERACTIONS:

PCAF (p300/CBP-associated factor) interacts with GATA zinc finger domain containing 2A (GATAD2A), mRNA, IgG (GenWay Catalog #: 18-003-43822).

PCAF (p300/CBP-associated factor) interacts with GATAD2B (GATA zinc finger domain containing 2B), IgG (GenWay Catalog #: 18-003-43822).

PCAF (p300/CBP-associated factor) interacts with Hif-1 alpha, Protein (GenWay Catalog #: 18-003-43822).

PCAF (p300/CBP-associated factor) interacts with HIF1 alpha [mgc3], IgG (GenWay Catalog #: 18-003-43822).

PCAF (p300/CBP-associated factor) interacts with HIF-1 Alpha Human, Protein (GenWay Catalog #: 18-003-43822).

PCAF (p300/CBP-associated factor) interacts with SIRT2 (sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)), IgG (GenWay Catalog #: 18-003-43822).

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

BACKGROUND REFERENCES for PCAF ANTIBODY:

Background references for antibody target are not specific to GenWay products

Chu.B.Y.. Biochem. J. 392 (PT 3). 589-599 (2005).

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: PCAF antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of PCAF antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about PCAF antibody, all related information, articles, resources about PCAF antibody are being stored on our online database. Let us know if you have questions regarding this product.

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