CASP3 antibody, CASP3 (caspase 3. apoptosis-related cysteine peptidase) Antibody, Rabbit Polyclonal Antibody

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Peptides \| Secondary Antibodies \| ELISA Kits

CASP3 (caspase 3. apoptosis-related cysteine peptidase) Antibody, Rabbit Polyclonal Antibody

Catalog Number: 18-003-44327

Buy CASP3 antibody -

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Related Product Names:

- CASP3 antibody; CASP3; CPP32, CPP32B, SCA-1; CASP3 (caspase 3. apoptosis-related cysteine peptidase)
- EC 3.4.22.56; CASP-3; Apopain; Cysteine protease CPP32; Yama protein; CPP-32; SREBP cleavage activity 1; SCA-1; Caspase 3 preproprotein; Caspase-3

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: CASP3	Gene Name Synonym: CPP32, CPP32B, SCA-1
Gi #: 14790119	NCBI Acc #: NP_004337.2	Swiss Prot Acc #: P42574
Length (aa): 277	Mol. Weight (Da): 31608	Chrom Location: N/A

Antigen Specificity: Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human CASP3.

ELISA Titre: 1:62500

Category: Tumor Suppressor Genes & Apoptosis

Note: Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity.

Source/Host: Rabbit

Purity/Purification: Rabbit IgG purified by protein A chromatography.

Clonality: Polyclonal

Crossreactivity: Human

Format: Suggested dilution at 5 microg/ml in 5% skim milk / PBS buffer. and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50.000 - 100.000 as second antibody.

Storage: Antibody is lyophilized from PBS buffer with 2% sucrose. Add 100 microl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage. store at -20C.

Stability: Note: Avoid repeat freeze-thaw cycles.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for CASP3 ANTIBODY:

WB: Tested

TESTING: (secondary reagents and protocols [IHC Protocol])

WB: ( anti-CASP3 )

CASP3 antibody WB image
CASP3

CASP3 ANTIBODY TARGET DESCRIPTION:

Synonym Names for CASP3 antibody: CASP3; CPP32, CPP32B, SCA-1; EC 3.4.22.56; CASP-3; Apopain; Cysteine protease CPP32; Yama protein; CPP-32; SREBP cleavage activity 1; SCA-1; Caspase 3 preproprotein; Caspase-3

CASP3 is a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic

Function: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.

Catalytic Activity: Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp-|- with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position.

Enzyme Regulation: Inhibited by isatin sulfonamides.

Subunit: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit.

Subcellular Location: Cytoplasm.

Tissue Specificity: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.

Ptm: Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.

Similarity: Belongs to the peptidase C14 family [view classification].

Summary: This protein is a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

CASP3 (caspase 3. apoptosis-related cysteine peptidase) reacts with human.

OMIM: 600636; gene. [NCBI / EBI]

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PRODUCT INTERACTIONS:

CASP3 (caspase 3. apoptosis-related cysteine peptidase) interacts with Baculoviral IAP repeat-containing protein 2, IgG (GenWay Catalog #: 18-003-44327).

CASP3 (caspase 3. apoptosis-related cysteine peptidase) interacts with cIAP1, IgG (GenWay Catalog #: 18-003-44327).

CASP3 (caspase 3. apoptosis-related cysteine peptidase) interacts with Baculoviral IAP repeat-containing protein 7, IgG (GenWay Catalog #: 18-003-44327).

CASP3 (caspase 3. apoptosis-related cysteine peptidase) interacts with LIVIN, IgG (GenWay Catalog #: 18-003-44327).

CASP3 (caspase 3. apoptosis-related cysteine peptidase) interacts with Livin [88C570], IgG (GenWay Catalog #: 18-003-44327).

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

BACKGROUND REFERENCES for CASP3 ANTIBODY:

Background references for antibody target are not specific to GenWay products

Selvakumar.P.. et al.. (2006) FEBS Lett. 580 (8). 2021-2026.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: CASP3 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of CASP3 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about CASP3 antibody, all related information, articles, resources about CASP3 antibody are being stored on our online database. Let us know if you have questions regarding this product.

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