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| NANOG (Nanog homeobox) Antibody, Rabbit Polyclonal Antibody |
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| Catalog Number: 18-003-44669 |
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| Trial Size Available. Please expect delivery of trial size products to be 4 weeks. |
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| Related Product Names:
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NANOG antibody; NANOG; NANOG (Nanog homeobox)
- Homeobox transcription factor Nanog; hNanog; Nanog homeobox; Homeobox protein NANOG |
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- Gene Information -
Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.
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| Gene Name: NANOG |
Gene Name Synonym: N/A |
Gi #:
153945816 |
NCBI Acc #:
NP_079141.2 |
Swiss Prot Acc #:
Q6JZS5 |
Length (aa): 305 |
Mol. Weight (Da): 34620 |
Chrom Location: N/A |
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 Antigen Specificity: Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human NANOG.
ELISA Titre: 1:1562500
Note: Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity.
Source/Host: Rabbit
Purity/Purification: Rabbit IgG purified by protein A chromatography.
Clonality: Polyclonal
Crossreactivity: Human
Format: Suggested dilution at 1.25 microg/ml in 5% skim milk / PBS buffer. and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50.000 - 100.000 as second antibody.
Storage: Antibody is lyophilized from PBS buffer with 2% sucrose. Add 100 microl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage. store at -20C.
Stability: Note: Avoid repeat freeze-thaw cycles.
Shipping: Products may be shipped on ice pack or dry ice. |
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| APPLICATIONS for NANOG ANTIBODY: |
| WB: Tested |
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| NANOG ANTIBODY TARGET DESCRIPTION: |
Synonym Names for NANOG antibody: NANOG; Homeobox transcription factor Nanog; hNanog; Nanog homeobox; Homeobox protein NANOG
NANOG is a new marker for testicular carcinoma in situ and germ cell tumors. Gene knockdown of Nanog promotes differentiation. thereby demonstrating a role for these factors in human embryonic stem cell self-renewal.
Function: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation (By similarity).
Subunit: Interacts with SMAD1 and SALL4 (By similarity).
Subcellular Location: Nucleus.
Tissue Specificity: Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
Developmental Stage: Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
Miscellaneous: Exists an other tandem duplicated non-processed pseudogene (NANOGP1) and 10 other NANOG-related nucleotide sequences located on different chromosomes, all of which are processed pseudogenes lacking introns (NANOGP2 to NANOGP11); except NANOGP8 which is a retrogene.
Similarity: Belongs to the Nanog homeobox family.
Similarity: Contains 1 homeobox DNA-binding domain.
NANOG (Nanog homeobox) reacts with human.
OMIM: 607937; gene. [NCBI / EBI]
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SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.
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| BACKGROUND
REFERENCES for NANOG ANTIBODY: |
| Background references for antibody target are not specific to GenWay products |
| Piestun.D.. et al.. (2006) Biochem. Biophys. Res. Commun. 343 (1). 279-285. |
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Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.
Precautions: NANOG antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.
Important Notes: During shipment, small volumes of NANOG antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.
Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about NANOG antibody, all related information, articles, resources about NANOG antibody are being stored on our online database. Let us know if you have questions regarding this product.
Disclaimer: For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed. |
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Thank you for visiting GenWay Biotech's Datasheet. GenWay is the world's leader in chicken, IgY antibody production, producing over 900 quality polyclonal IgY antibodies to date. From our unique recombinant protein production, utilizing a variety of protein expression systems, GenWay's polyclonal IgY antibodies incorporate more epitopes, increasing the success rate for ELISA, western blot, immunohistochemistry (IHC), immunocytochemistry (ICC), and many more immunoassays. |
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