KITLG protein, SCF Rat recombinant protein

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Peptides \| Secondary Antibodies \| ELISA Kits

SCF Rat recombinant protein

Catalog Number: 10-663-45160

Buy KITLG protein -

Size:

Related Product Names:

- KITLG protein; KITLG; MGF; SCF; SCF Rat
- Kit ligand Precursor, C-kit ligand, SCF, Mast cell growth factor, MGF, SF, KL-1, Kitl, DKFZp686F2250, Hematopoietic growth factor KL.; C-kit ligand; Stem cell factor; SCF; Mast cell growth factor; MGF; kit ligand isoform 1 [Rattus norvegicus]

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: KITLG	Gene Name Synonym: MGF; SCF
Gi #: N/A	NCBI Acc #: NP_000890.1	Swiss Prot Acc #: P21583
Length (aa): N/A	Mol. Weight (Da): 30899	Chrom Location: N/A

Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Glu-Ile-Cys.
MQEICRNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV THLSVSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVAC MEENAPKNVK ESLKKPETRN FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA.

Biological Activity: The ED50 is determined by the dose-dependant stimulation of the proliferation of human TF-1 cells which is < 10 ng/ml, corresponding to a specific activity of > 1 x 10⁵ units/mg.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Solubility: It is recommended to reconstitute the lyophilized Stem cell factor in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source/Host: E. Coli

Purity/Purification: Greater than 98.0% as determined by (a)Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Format: Lyophilized from a concentrated solution in water containing 0.02% NaHCO₃.

Stability: Lyophilized rat SCF although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution SCF should be stored at 4 C between 2-7 days and for future use below -18 C.
For long term storage it is recommended to add a carier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Shipping: Products may be shipped on ice pack or dry ice.

TESTING: (secondary reagents and protocols )

Not Available

KITLG PROTEIN TARGET DESCRIPTION:

Synonym Names for KITLG protein: KITLG; MGF; SCF; Kit ligand Precursor, C-kit ligand, SCF, Mast cell growth factor, MGF, SF, KL-1, Kitl, DKFZp686F2250, Hematopoietic growth factor KL.; C-kit ligand; Stem cell factor; SCF; Mast cell growth factor; MGF; kit ligand isoform 1 [Rattus norvegicus]

Stem cell factor Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 164 amino acids (26-189) and having a molecular mass of 18.4 kDa. The Rat SCF is purified by proprietary chromatographic techniques.

Stem cell factor / KIT ligand (SCF) is a cytokine which binds CD117(c-Kit). SCF is also known as "steel factor" or "c-kit ligand". SCF exists in two forms, cell surface bound SCF and soluble (or free) SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases.
SCF is a growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and other hematopoietic progenitor cells. One of its roles is to change the BFU-E (burst-forming unit-erythroid) cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E (colony-forming unit-erythroid).

Function: Stimulates the proliferation of mast cells. Able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. Mediates also cell-cell adhesion. Acts synergistically with other cytokines, probably interleukins.

Subunit: Homodimer, non-covalently linked (Probable).

Subcellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein (By similarity). Secreted (By similarity). Note=Also exists as a secreted soluble form (isoform 1 only) (By similarity).

Subcellular Location: Isoform 2: Cell membrane; Single-pass type I membrane protein (By similarity). Cytoplasm, cytoskeleton (By similarity).

Developmental Stage: Acts in the early stages of hematopoiesis.

Ptm: A soluble form is produced by proteolytic processing of isoform 1 in the extracellular domain.

Ptm: Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.

Polymorphism: Genetic variations in KITLG are associated with variation in skin/hair/eye pigmentation type 7 (SHEP7) [MIM:611664]. Hair, eye and skin pigmentation are among the most visible examples of human phenotypic variation, with a broad normal range that is subject to substantial geographic stratification. In the case of skin, individuals tend to have lighter pigmentation with increasing distance from the equator. By contrast, the majority of variation in human eye and hair color is found among individuals of European ancestry, with most other human populations fixed for brown eyes and black hair.

Similarity: Belongs to the SCF family.

OMIM: 184745; gene. [NCBI / EBI]
611664; phenotype. [NCBI / EBI]

Pathways:
KEGG pathway: Cytokine-cytokine receptor interaction 04060
KEGG pathway: Hematopoietic cell lineage 04640
KEGG pathway: Melanogenesis 04916

Products similar to KITLG protein:

IgG

Rabbit anti Human SCF, IgG

STAT5 phospho Y694, IgG

SCF [1.2_2H5-1C10], IgG

Stat5a, IgG

c-Kit phospho Y730, IgG

Protein

Mouse Stem Cell Factor, Protein

SCF Human, Protein

SCF (Stem Cell Factor), Protein

Human Stem Cell Factor,, Protein

STEM CELL FACTOR, Protein

PRODUCT INTERACTIONS:

SCF Rat interacts with CD117, IgG (GenWay Catalog #: 10-663-45160).

SCF Rat interacts with cKit HUMAN, Protein (GenWay Catalog #: 10-663-45160).

SCF Rat interacts with c-Kit (Phospho-Tyr721), IgG (GenWay Catalog #: 10-663-45160).

SCF Rat interacts with CD117, IgG (GenWay Catalog #: 10-663-45160).

SCF Rat interacts with Rat Anti-Mouse CD117/c-Kit, IgG (GenWay Catalog #: 10-663-45160).

SCF Rat interacts with c-Kit [104D2] (PE), IgG (GenWay Catalog #: 10-663-45160).

SCF Rat interacts with c-Kit [104D2], IgG (GenWay Catalog #: 10-663-45160).

SCF Rat interacts with CD117, IgG (GenWay Catalog #: 10-663-45160).

SCF Rat interacts with c-Kit phospho Y730, IgG (GenWay Catalog #: 10-663-45160).

SCF Rat interacts with c-Kit phospho Y823, IgG (GenWay Catalog #: 10-663-45160).

SCF Rat interacts with c-Kit phospho Y936, IgG (GenWay Catalog #: 10-663-45160).

SCF Rat interacts with c-Kit phospho Y568, Y570, IgG (GenWay Catalog #: 10-663-45160).

SCF Rat interacts with CD117 [2B8], IgG (GenWay Catalog #: 10-663-45160).

SCF Rat interacts with c-Kit (ready-to-use), IgG (GenWay Catalog #: 10-663-45160).

SCF Rat interacts with C-KIT (CD117), C-Term, IgG (GenWay Catalog #: 10-663-45160).

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Precautions: KITLG protein is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of KITLG protein vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about KITLG protein, all related information, articles, resources about KITLG protein are being stored on our online database. Let us know if you have questions regarding this product.

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