IL10 protein, IL-10 Porcine recombinant protein

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IL-10 Porcine recombinant protein

Catalog Number: 10-663-45231

Buy IL10 protein -

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Related Product Names:

- IL10 protein; IL10; IL-10 Porcine
- B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor.; IL-10; Cytokine synthesis inhibitory factor; CSIF; Interleukin-10

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: IL10	Gene Name Synonym: N/A
Gi #: N/A	NCBI Acc #: NP_000563.1	Swiss Prot Acc #: P22301
Length (aa): N/A	Mol. Weight (Da): 20517	Chrom Location: N/A

Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Ile-Lys-Ser.

Biological Activity: The ED50 as determined by the dose-dependant stimulation of MC/9 cell which was found to be < 15ng/ml.

Protein Content: Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.36 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IL-10 as a Reference Standard.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Solubility: It is recommended to reconstitute the lyophilized Interleukin 10 in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source/Host: E. Coli

Purity/Purification: Greater than 95.0% as determined by (a)Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Format: The protein was lyophilized with no additives.

Stability: Lyophilized Interleukin-10 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution IL10 should be stored at 4 C between 2-7 days and for future use below -18 C.
For long term storage it is recommend

Shipping: Products may be shipped on ice pack or dry ice.

TESTING: (secondary reagents and protocols )

Not Available

IL10 PROTEIN TARGET DESCRIPTION:

Synonym Names for IL10 protein: IL10; B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor.; IL-10; Cytokine synthesis inhibitory factor; CSIF; Interleukin-10

Interleukin-10 Porcine Recombinant produced in E.coli is a single, glycosylated polypeptide chain containing 160 amino acids and having a molecular mass of 18458 Dalton.
The IL-10 is purified by proprietary chromatographic techniques.

IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.

Function: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.

Subunit: Homodimer.

Subcellular Location: Secreted.

Tissue Specificity: Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.

Disease: Defects in IL10 are a cause of susceptibility to Crohn disease (CD) [MIM:266600]. CD is a form of remitting inflammatory bowel disease (IBD). CD may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous. Crohn disease is commonly classified as autoimmune disease.

Similarity: Belongs to the IL-10 family.

OMIM: 124092; gene. [NCBI / EBI]
266600; phenotype. [NCBI / EBI]

Pathways:
KEGG pathway: Cytokine-cytokine receptor interaction 04060
KEGG pathway: Jak-STAT signaling pathway 04630
KEGG pathway: T cell receptor signaling pathway 04660

Products similar to IL10 protein:

IgG

IL10 [23738.11], IgG

IL10, IgG

IL10 [JES5-2A5], IgG

IL10 [JES3-9D7], IgG

INTERLEUKIN 10, IgG

PRODUCT INTERACTIONS:

IL-10 Porcine interacts with IL10RA [1B1.3a] (PE), IgG (GenWay Catalog #: 10-663-45231).

IL-10 Porcine interacts with IL10RA [1B1.3a], IgG (GenWay Catalog #: 10-663-45231).

IL-10 Porcine interacts with Human IL-10 R alpha-FcA Chimera, Protein (GenWay Catalog #: 10-663-45231).

IL-10 Porcine interacts with IL10RA (interleukin 10 receptor. alpha), IgG (GenWay Catalog #: 10-663-45231).

IL-10 Porcine interacts with CD210, IgG (GenWay Catalog #: 10-663-45231).

IL-10 Porcine interacts with IL10RA [37607.11], IgG (GenWay Catalog #: 10-663-45231).

SUGGESTED PROTEIN REAGENTS - GenWay offers reagents to facilitate your research with cell-based assays, Mass Spectrometry, standards, calibrators, Western blot, cell culture, antibiotics, microwell substrates, and more!

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: IL10 protein is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of IL10 protein vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about IL10 protein, all related information, articles, resources about IL10 protein are being stored on our online database. Let us know if you have questions regarding this product.

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