GPT protein, ALAT Human recombinant protein

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ALAT Human recombinant protein

Catalog Number: 10-663-45306

Buy GPT protein -

Units:

Related Product Names:

- GPT protein; GPT; AAT1; GPT1; ALAT Human
- ALT1, Glutamic-pyruvic transaminase 1, GPT 1, Glutamic-alanine transaminase 1, AAT1, ALT, ALAT, SGPT.; ALT1; EC 2.6.1.2; Glutamic--pyruvic transaminase 1; Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Alanine aminotransferase 1

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: GPT	Gene Name Synonym: AAT1; GPT1
Gi #: N/A	NCBI Acc #: NP_005300.1	Swiss Prot Acc #: P24298
Length (aa): N/A	Mol. Weight (Da): 54637	Chrom Location: N/A

Biological Activity: The specific activity was found to be 1000 U/mg.

Physical Appearance: Sterile liquid formulation at a concentration of 1,7200U/ml

Formulation: The protein (1,7200U/ml) was dialyzed against 40mM sodium acetate buffer (pH 5.5), 1mM DTT, 1 mM EDTA, 5mM 2-oxoglutarate and 0.1mM pyridoxal-5'-phosphate

Source/Host: E. Coli

Purity/Purification: Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Format: The protein was dialyzed against 40mM sodium acetate buffer (pH 5.5), 1mM DTT,1mM EDTA, 5mM 2-oxoglutarate and 0.1mM pyridoxal-5'-phosphate.

Stability: AAT1 although stable at 10 C for 5 days, should be stored desiccated below -18 C.
Please prevent freeze-thaw cycles.

Shipping: Products may be shipped on ice pack or dry ice.

TESTING: (secondary reagents and protocols )

Not Available

GPT PROTEIN TARGET DESCRIPTION:

Synonym Names for GPT protein: GPT; AAT1; GPT1; ALT1, Glutamic-pyruvic transaminase 1, GPT 1, Glutamic-alanine transaminase 1, AAT1, ALT, ALAT, SGPT.; ALT1; EC 2.6.1.2; Glutamic--pyruvic transaminase 1; Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Alanine aminotransferase 1

Alanine Aminotransferase Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing having a molecular mass of 54,479 Dalton.
The ALT is purified by proprietary chromatographic techniques.

Alanine transaminase or ALT is a transaminaseenzyme.
ALT is found in serumand in various bodily tissues, but is most commonly associated with the liver; It catalyzes the transfer of an aminogroup from alanineto a-ketoglutarate, the products of this reversible transaminationreaction being pyruvateand glutamate:
alanine+ ?-ketoglutarate? pyruvate+ glutamate
It is commonly measured clinically as a part of a diagnostic liver function test, to determine liver health. It is also called serum glutamate pyruvate transaminase (SGPT) or alanine aminotransferase (ALAT). Diagnostically, it is almost always measured in units/litre (U/L).

Function: Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.

Catalytic Activity: L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.

Cofactor: Pyridoxal phosphate.

Pathway: Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.

Subunit: Homodimer.

Subcellular Location: Cytoplasm.

Tissue Specificity: Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue.

Induction: By glucocorticoids.

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.

OMIM: 138200; gene. [NCBI / EBI]

Pathways:
KEGG pathway: Alanine and aspartate metabolism 00252
KEGG pathway: Carbon fixation 00710
KEGG pathway: Glutamate metabolism 00251
Reactome Event:Metabolism of amino acids 71291

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Precautions: GPT protein is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of GPT protein vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about GPT protein, all related information, articles, resources about GPT protein are being stored on our online database. Let us know if you have questions regarding this product.

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