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| T4 DNA recombinant protein |
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| Catalog Number: 10-663-45311 |
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| Related Product Names:
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LIG4 protein; LIG4; T4 DNA
- DNA ligase 4, EC 6.5.1.1, DNA ligase IV, Polydeoxyribonucleotide synthase [ATP] 4.; EC 6.5.1.1; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4 |
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- Gene Information -
Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.
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| Gene Name: LIG4 |
Gene Name Synonym: N/A |
Gi #:
N/A |
NCBI Acc #:
NP_001091738.1 |
Swiss Prot Acc #:
P49917 |
Length (aa): N/A |
Mol. Weight (Da): 103971 |
Chrom Location: N/A |
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 Biological Activity: One Weiss unit is equivalent to circa 67 cohesive-end ligation units.
T4 DNA Ligase is strongly inhibited by NaCl or KCl if the concentration is > 200mM.
·Ligation of blunt-ended and single-base pair overhang fragments requires about 50 times as much enzyme to achieve the same extent of ligation as cohesive-end DNA fragments. Blunt-end ligation may be enhanced by addition of PEG 4000 (10% w/v final concentration) or hexamine chloride, or by reducing the ATP concentration to 50uM.
·To dilute T4 DNA Ligase that will subsequently be stored at –200C, 50% glycerol storage buffer should be used; to dilute for immediate use, 1x T4 DNA Ligase reaction buffer can be used.
Physical Appearance: Sterile filtered liquid formulation having a concentration of 167,000 U/ml.
Unit Definition: One unit is defined as the amount of enzyme required to give 50% ligation of Hind III fragments of DNA (5´ DNA termini concentration of 0.12 uM, 300- ug/ml) in a total reaction volume of 20 ul in 30 minutes at 16 C in 1X T4 DNA Ligase Reaction Buffer.
2.One Weiss unit is defined as the amount of enzyme required to catalyze the exchange of 1 nmol of 32P from pyrophosphate to ATP, into Norit-adsorbable material in 20 minutes at 37 C.
Useful Applications: Cloning of restriction fragments.
Joining linkers and adapters to blunt-ended DNA
Source/Host: E. Coli (lambda lysogen NM 989)
Format: 50mM Tris-HCl (pH 7.8 at 25OC), 10mM MgCl2, 10mM DTT, 1mM ATP, 25 ug/ml BSA and DNA (0.1 to 1 uM in 5´ termini). ?ptimal ligation occurs at 16oC.
Stability: Two years when stored at –20 C, 2 weeks at 4 C.
Shipping: Products may be shipped on ice pack or dry ice. |
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| LIG4 PROTEIN TARGET DESCRIPTION: |
Synonym Names for LIG4 protein: LIG4; DNA ligase 4, EC 6.5.1.1, DNA ligase IV, Polydeoxyribonucleotide synthase [ATP] 4.; EC 6.5.1.1; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5? -phosphate and 3? -hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.
Function: Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
Catalytic Activity: ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Subunit: Binds to XRCC4. The LIG4-XRCC4 complex has probably a 1:2 stoichiometry. The LIG4-XRCC4 heteromer associates in a DNA-dependent manner with the DNA-dependent protein kinase complex DNA-PK, formed by the Ku p70/p86 dimer (G22P1/G22P2) and PRKDC.
Subcellular Location: Nucleus.
Tissue Specificity: Testis, thymus, prostate and heart.
Disease: Defects in LIG4 are the cause of LIG4 syndrome [MIM:606593]. This disease is characterized by immunodeficiency and developmental and growth delay. Patients display unusual facial features, microcephaly, growth and/or developmental delay, pancytopenia, and various skin abnormalities.
Disease: Defects in LIG4 are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-positive with sensitivity to ionizing radiation (RSSCID) [MIM:602450]. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. Individuals affected by RS-SCID show defects in the DNA repair machinery necessary for coding joint formation and the completion of V(D)J recombination. A subset of cells from such patients show increased radiosensitivity.
Similarity: Belongs to the ATP-dependent DNA ligase family.
Similarity: Contains 2 BRCT domains.
OMIM: 601837; gene. [NCBI / EBI]
602450; phenotype. [NCBI / EBI]
606593; phenotype. [NCBI / EBI]
Pathways:
Reactome Event:DNA Repair 73894
Reactome Event:HIV Infection 162906
Products similar to LIG4 protein:
IgG
 LIG4 (ligase IV. DNA. ATP-dependent), IgG
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| PRODUCT INTERACTIONS: |
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SUGGESTED PROTEIN REAGENTS - GenWay offers reagents to facilitate your research with cell-based assays, Mass Spectrometry, standards, calibrators, Western blot, cell culture, antibiotics, microwell substrates, and more!
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Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.
Precautions: LIG4 protein is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.
Important Notes: During shipment, small volumes of LIG4 protein vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.
Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about LIG4 protein, all related information, articles, resources about LIG4 protein are being stored on our online database. Let us know if you have questions regarding this product.
Disclaimer: For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed. |
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