CENPA protein, CENP-A Human recombinant protein

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Peptides \| Secondary Antibodies \| ELISA Kits

CENP-A Human recombinant protein

Catalog Number: 10-663-45663

Buy CENPA protein -

Size:

Trial Size Available.

Related Product Names:

- CENPA protein; CENPA; CENP-A Human
- Centromere Protein A Human Recombinant; Centromere protein A; CENP-A; Centromere autoantigen A; histone H3-like centromeric protein A isoform b

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: CENPA	Gene Name Synonym: N/A
Gi #: N/A	NCBI Acc #: NP_001035891.1	Swiss Prot Acc #: P49450
Length (aa): N/A	Mol. Weight (Da): 15991	Chrom Location: N/A

Coating Concentration: 0.15-0.4 ug/ml (depending on the type of ELISA plate and coating buffer).
Suitable for biotinylation and iodination.

Immunological Functions: 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).

Useful Applications: Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera.

Purity/Purification: Greater than 90% (SDS-PAGE).

Format: Recombinant Human CENP-A is supplied at a of 0.6mg/ml in 20mM HEPES buffer pH-8, 100mM sodium chloride, and 6M Urea.

Storage: Store at 4 C if entire vial will be used within 2-4 weeks. Store, frozen at -20 C for longer periods of time. Avoid multiple freeze-thaw cycles.

Shipping: Products may be shipped on ice pack or dry ice.

TESTING: (secondary reagents and protocols )

Not Available

CENPA PROTEIN TARGET DESCRIPTION:

Synonym Names for CENPA protein: CENPA; Centromere Protein A Human Recombinant; Centromere protein A; CENP-A; Centromere autoantigen A; histone H3-like centromeric protein A isoform b

Centromere proteins are a group of proteins which form and/or mediate the function of centromeres, the central structures of chromosomes to which spindle fibers/microtubuli attach and pull the chromosomes apart in cell division. Currently, 9 centromere proteins are known and designated CENP-A to CENP-I. Most of the centromere proteins are targets of autoantibodies, the anti-centromere antibodies.
CENP-A is another important centromeric autoantigen in addition to CENP-B: it has a molecular weight of approx. 20 kDa and is incorporated into centromeric chromatin due to its histone-like properties. CENP-A antibodies are an important marker for correct diagnosis of Scleroderma / CREST syndrome in CENP-B-autoantibody negative patients. Recombinant Human CENP-A produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 17,015 dalton.
rHuCENP-A is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.

Function: Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division.

Subunit: Forms a nucleosome-like histone octamer containing two molecules each of H2A, H2B, CENPA and H4 assembled in one CENPA-H4 heterotetramer and two H2A-H2B heterodimers. Nucleosomes containing CENPA also contain histone H2A variants such as macroH2A H2AFY and H2A.Z/H2AFZ. The CENPA-H4 heterotetramer is more compact and structurally more rigid than corresponding H3-H4 heterotetramers. Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and MLF1IP/CENPU. Interacts directly with herpes virus HSV-1 ICP0 protein.

Subcellular Location: Nucleus. Centromere. Kinetochore. Note=Localizes exclusively in the kinetochore domain of centromeres.

Domain: The CATD (CENPA targeting domain) region is responsible for the more compact structure of nucleosomes containing CENPA and is necessary and sufficient to mediate the localization into centromeres.

Ptm: Ubiquitinated (Probable). Interaction with herpes virus HSV-1 ICP0 protein, leads to its degradation by the proteasome pathway.

Ptm: Phosphorylation of Ser-7 by Aurora-A/STK6 and Aurora-B/STK12 during prophase is required for localization of Aurora-A/STK6 and Aurora-B/STK12 at inner centromere and is essential for kinetochore function. Initial phosphorylation during prophase is mediated by Aurora-A/STK6 and is maintained by Aurora-B/STK12.

Disease: Antibodies against CENPA are present in sera from patients with autoimmune diseases that developed autoantibodies against centrosomal proteins.

Similarity: Belongs to the histone H3 family.

OMIM: 117139; gene. [NCBI / EBI]

Products similar to CENPA protein:

IgG

CENPA [3-19], IgG

PRODUCT INTERACTIONS:

CENP-A Human interacts with c-Abl phospho Y245, IgG (GenWay Catalog #: 10-663-45663).

CENP-A Human interacts with c-Abl (Phospho-Tyr412), IgG (GenWay Catalog #: 10-663-45663).

CENP-A Human interacts with c-Abl (Ab-412), IgG (GenWay Catalog #: 10-663-45663).

CENP-A Human interacts with c-Abl (Phospho-Tyr412) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 10-663-45663).

CENP-A Human interacts with Herpes Type 6 p150, IgG (GenWay Catalog #: 10-663-45663).

CENP-A Human interacts with c-Abl (ready-to-use), IgG (GenWay Catalog #: 10-663-45663).

CENP-A Human interacts with c-Abl, IgG (GenWay Catalog #: 10-663-45663).

CENP-A Human interacts with c-Abl [19-110], IgG (GenWay Catalog #: 10-663-45663).

CENP-A Human interacts with c-Abl [ABL-148], IgG (GenWay Catalog #: 10-663-45663).

CENP-A Human interacts with c-Abl phospho Y412, IgG (GenWay Catalog #: 10-663-45663).

CENP-A Human interacts with c-Abl, IgG (GenWay Catalog #: 10-663-45663).

CENP-A Human interacts with FYN, IgG (GenWay Catalog #: 10-663-45663).

CENP-A Human interacts with FYN [FYN-01], IgG (GenWay Catalog #: 10-663-45663).

CENP-A Human interacts with Fyn-p59 Human, Protein (GenWay Catalog #: 10-663-45663).

CENP-A Human interacts with FYN, IgG (GenWay Catalog #: 10-663-45663).

CENP-A Human interacts with Fyn (p59Fyn), IgG (GenWay Catalog #: 10-663-45663).

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Precautions: CENPA protein is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of CENPA protein vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about CENPA protein, all related information, articles, resources about CENPA protein are being stored on our online database. Let us know if you have questions regarding this product.

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