Alphabetical Listing of Primary Antibodies:
f
|
0-4
|
5-9
|
A
|
B
|
C
|
D
|
E
|
F
|
G
|
H
|
I
|
J
|
K
|
L
|
M
|
N
|
O
|
P
|
Q
|
R
|
S
|
T
|
U
|
V
|
W
|
X
|
Y
|
Z
|
Alphabetical Listing of Proteins:
f
|
0-4
|
5-9
|
A
|
B
|
C
|
D
|
E
|
F
|
G
|
H
|
I
|
J
|
K
|
L
|
M
|
N
|
O
|
P
|
Q
|
R
|
S
|
T
|
U
|
V
|
W
|
X
|
Y
|
Z
|
Peptides
|
Secondary Antibodies
|
ELISA Kits
Ku P70/P80 Human recombinant protein
Catalog Number: 10-663-45671
Buy XRCC6 protein -
Size:
0.005 mg ($80.00)
0.02 mg ($220.00)
1 mg ($4,700.00)
Related Product Names:
- XRCC6 protein; XRCC6; G22P1; Ku P70/P80 Human
- Ku P70/P80 Human Recombinant; ATP-dependent DNA helicase II 70 kDa subunit; Lupus Ku autoantigen protein p70; Ku70; 70 kDa subunit of Ku antigen; Thyroid-lupus autoantigen; TLAA; CTC box-binding factor 75 kDa subunit; CTCBF; CTC75; DNA repair protein XRCC; ATP-dependent DNA helicase 2 subunit 1
- Gene Information -
Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact
sales@genwaybio.com
.
Gene Name:
XRCC6
Gene Name Synonym:
G22P1
Gi #:
N/A
NCBI Acc #:
NP_001460.1
Swiss Prot Acc #:
P12956
Length (aa):
N/A
Mol. Weight (Da):
69843
Chrom Location:
N/A
Physical Appearance:
Sterile Filtered clear solution.
Coating Concentration:
0.2-0.6 ug/ml (depending on the type of ELISA plate and coating buffer).
Suitable for biotinylation and iodination.
Immunological Functions:
1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).
Useful Applications:
Western-Blot with monoclonal anti-hexa-His-tag antibody & anti-Ku autoantibody-positive patient sera.
Source/Host:
Insect (Sf9 cells)
Purity/Purification:
Greater than 90% (SDS-PAGE).
Format:
Recombinant Human KuP70/P80is supplied at a of 1mg/ml in16mM HEPES buffer pH-8, 160mM NaCl, and 20% glycerol.
Storage:
Store at 4 C if entire vial will be used within 2-4 weeks. Store, frozen at -20 C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Shipping:
Products may be shipped on ice pack or dry ice.
TESTING: (
secondary reagents
and
protocols
)
Not Available
XRCC6 PROTEIN TARGET DESCRIPTION:
Synonym Names for XRCC6 protein:
XRCC6; G22P1; Ku P70/P80 Human Recombinant; ATP-dependent DNA helicase II 70 kDa subunit; Lupus Ku autoantigen protein p70; Ku70; 70 kDa subunit of Ku antigen; Thyroid-lupus autoantigen; TLAA; CTC box-binding factor 75 kDa subunit; CTCBF; CTC75; DNA repair protein XRCC; ATP-dependent DNA helicase 2 subunit 1
Ku antigen consists of two polypeptides (70 kDa and 80 kDa; the p80 subunit is occasionally called p86). The Ku heterodimer provides DNA-binding and regulatory functions to DNA-dependent protein kinase which is a central enzyme for DNA recombination processes in eucaryotic cells. The Ku protein plays important roles in multiple nuclear processes including DNA repair, V(D)J recombination, telomere maintenance, and the regulation of specific gene transcription.
Antibodies to Ku were originally reported in scleroderma-polymyositis overlap syndrome (up to 20% of patients) but have also been detectable in about 20 % of patients with primary pulmonary hypertension (as a complication of overlap), in 5-10 % of SLE and MCTD patients, and in up to 20 % of patients with primary Sjögren's syndrome.
In IIF using HEp-2 cells, Ku-positive sera yield a dense, fine granular pattern with nucleolar staining that is typical but not specific for anti-Ku. Detection of Ku antibodies by Western blot may bear false-negative results since the main reactive epitope on the p70/p80 heterodimer is conformation-dependent. Recombinant Human KuP70/P80 produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 43,591 dalton. rhKu(P70/P80) is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.
Function:
Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by p70. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The Ku p70/p86 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The Ku p70/p86 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression (By similarity).
Subunit:
Heterodimer of a 70 kDa and a 80 kDa subunit. The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex. The dimer also associates with NARG1, and this complex binds to the osteocalcin promoter and activates osteocalcin expression. In addition, the 70 kDa subunit binds to the osteoblast-specific transcription factors MSX2, RUNX2 and DLX5. Interacts with ELF3. Interactis with XRCC6BP1.
Subcellular Location:
Nucleus.
Developmental Stage:
Expression does not increase during promyelocyte differentiation.
Induction:
In osteoblasts, by FGF2.
Ptm:
Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair.
Disease:
Individuals with systemic lupus erythematosus (SLE) and related disorders produce extremely large amounts of autoantibodies to p70 and p86. Existence of a major autoantigenic epitope or epitopes on the C-terminal 190 amino acids of p70 containing the leucine repeat. The majority of autoantibodies to p70 in most sera from patients with SLE seem to be reactive with this region.
Similarity:
Contains 1 Ku domain.
Similarity:
Contains 1 SAP domain.
OMIM:
152690; gene. [
NCBI
/
EBI
]
Pathways:
Reactome Event:DNA Repair
73894
Reactome Event:HIV Infection
162906
Products similar to XRCC6 protein:
IgG
KU70 / XRCC6, IgG
X-ray repair complementing defective repair in Chinese hamster cells 6 (Ku autoantigen, 70kDa), IgG
PSE1/Ku Protein, IgG
G22P1, IgG
Ku70 + Ku80 [162], IgG
PRODUCT INTERACTIONS:
Ku P70/P80 Human
interacts
with
BARD1, IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
Huntingtin, IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
Huntingtin (a.a. 1844-2131), IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
Huntingtin (a.a. 2703-2911), IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
Huntingtin (a.a. 997-1276), IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
Anti-Huntington pS421, IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
HUNTINGTIN, IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
HUNTINGTIN, IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
HUNTINGTIN, IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
NDRG1 (N-myc downstream regulated gene 1), IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
NDRG1 (N-myc downstream regulated gene 1), IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
Cellular tumor antigen p53, IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
Cellular tumor antigen p53, IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 Human, Protein
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53M Human, Protein
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53, IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 [SP5], IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53, IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 phospho S15, IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 [DO-13], IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 [DO-7], IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 [Pab240], IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 [D01], IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 [3F301], IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 [DO-7] (ready-to-use), IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 [BP53-12], IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
I-kappaB-alpha (Phospho-Ser32/Ser36), IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 (Phospho-Ser15), IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 (Phospho-Ser46), IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 (Phospho-Ser315), IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 (Ab-15), IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 (Ab-46), IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 (Ab-315), IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 (Phospho-Ser15) Antibody Blocking Peptide, Peptide
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 (Phospho-Ser46) Antibody Blocking Peptide, Peptide
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 (Phospho-Ser315) Antibody Blocking Peptide, Peptide
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53, IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
TP53 (tumor protein p53 (Li-Fraumeni syndrome)), IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 (a.a. 32-79), IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 (a.a. 88-109), IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 (a.a. 306-393), IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
Mouse p53, Wild Type, IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
Human p53 Oncogene, IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 [B20.1 (BP 53.122)] antibody (ready-to-use), IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 AIP1, IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 [BP53-12] (FITC), IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 [DO-2], IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 [DO-1], IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
P53, IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53, IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53, IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53, IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53, IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53, IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53, IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 [B20.1 (BP 53.122)] antibody (FITC), IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53, IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53, IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
P53 [SP5] (ready-to-use), IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 [DO-1], IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 [B449], IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 [Pab240], IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 [Pab1801], IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 [Pab1801], IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 [D01], IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53 [DO-2], IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53, IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53, IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53, IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
p53, IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
Ku80 [149.8], IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
Ku80 [149.8], IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
Ku80 [9403], IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
Ku80, IgG
(GenWay Catalog #: 10-663-45671)
.
Ku P70/P80 Human
interacts
with
Ku80 [9403], IgG
(GenWay Catalog #: 10-663-45671)
.
SUGGESTED PROTEIN REAGENTS
- GenWay offers reagents to facilitate your research with cell-based assays, Mass Spectrometry, standards, calibrators, Western blot, cell culture, antibiotics, microwell substrates, and more!
Order Confirmation:
Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.
Precautions:
XRCC6 protein is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.
Important Notes:
During shipment, small volumes of XRCC6 protein vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.
Copyright:
This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about XRCC6 protein, all related information, articles, resources about XRCC6 protein are being stored on our online database. Let us know if you have questions regarding this product.
Disclaimer:
For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed.
GenWay is a Protein and Antibody Solutions Provider. Customer Satisfaction is Our Top Priority.
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MYCBP (c-myc binding protein)
Myeloid leukemia factor 1
NANOG (Nanog homeobox)
NAP-2 Human
NFKB2 (nuclear factor of kappa light pol...
NFKB2 (nuclear factor of kappa light pol...
NOTCH3 (Notch homolog 3 (Drosophila))
OPG Human, His
OSM Human
P-Sel Human
p21 Human
P27 kip1 protein
P40
p53 Human
Parathyroid Hormone
PAX5 (paired box gene 5 (B-cell lineage ...
PCNA (proliferating cell nuclear antigen...
PCNA Human
PDGF-BB Human, Yeast
PDGF-BB Mouse
Peripheral-type benzodiazepine receptor
PfHSP70
PKC-a Human
PKC-d Human
PREB (prolactin regulatory element bindi...
PRL-R Bovine
PRL-R Ovine
Pro-epidermal growth factor
Prolactin
Prolactin Human
Prolactin Ovine
Prostate-specific antigen
Protein p65
PTEN Human
PTH 1-34 Human
PTH Human 1-84
Putative transmembrane receptor
Putative transmembrane receptor
Putative transmembrane receptor
RAC-alpha serine/threonine-protein kinas...
Rantes Human
RAP Rat
Ras-related C3 botulinum toxin substrate...
RB1 (retinoblastoma 1 (including osteosa...
RELA
Rhodopsin
RTSbeta
RUNX1T1 (runt-related transcription fact...
RUNX2 (runt-related transcription factor...
SARS Core 1-49
SARS Core 1-49, 192-220
SARS Core 340-390
SCF (Stem Cell Factor)
SCF Human
SCF Human, Sf9
SCF Mouse
SCF Rat
SDF-1a Human
SDF-1a Mouse
SDF-1b Human
SDF-1b Mouse
Serum amyloid P-component
Signal transducer and activator of trans...
SMAD3 (SMAD family member 3)
SPA
STAT1 Human
STAT3 Human
T-cell-specific surface glycoprotein CD2...
T.gondii p30
TAL1
TAL1 (T-cell acute lymphocytic leukemia ...
TATA-binding protein-associated phosphop...
TBP (TATA box binding protein)
TNFRI Human
TNFRSF10A (tumor necrosis factor recepto...
TOP1 Human
TP53 (tumor protein p53 (Li-Fraumeni syn...
tPA Human
TPO Human
TPO Human
TPO Mouse
TRIM5 alpha
TRIM5 alpha
TRIM5 alpha
Tripartite motif-containing 5 alpha isof...
Tripartite motif-containing 5 alpha isof...
TRX Yeast
Trypsin Bovine
Tumor necrosis factor receptor superfami...
Tumor necrosis factor receptor superfami...
Tyrosine-protein kinase BTK
Ubiquitin Human
VEGF (121) Human, Sf9
Vim Human
ZNFN1A1
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Protein
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Chicken
Equine
Feline
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Xenopus
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Xreactivity:
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Chicken
Drosophila
Equine
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Goat
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Human
Insect
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Viral
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Microbeads
RPE
Texas Red
TRITC
Specific Categories
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