NOG protein, Noggin Human recombinant protein

Alphabetical Listing of Primary Antibodies:	f\| 0-4 \| 5-9 \| A \| B \| C \| D \| E \| F \| G \| H \| I \| J \| K \| L \| M \| N \| O \| P \| Q \| R \| S \| T \| U \| V \| W \| X \| Y \| Z \|
Alphabetical Listing of Proteins:	f\| 0-4 \| 5-9 \| A \| B \| C \| D \| E \| F \| G \| H \| I \| J \| K \| L \| M \| N \| O \| P \| Q \| R \| S \| T \| U \| V \| W \| X \| Y \| Z \|
Peptides \| Secondary Antibodies \| ELISA Kits

Noggin Human recombinant protein

Catalog Number: 10-663-45804

Buy NOG protein -

Size:

Trial Size Available

Related Product Names:

- NOG protein; NOG; Noggin Human
- SYM1, SYNS1, NOG.; N/A; Noggin

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: NOG	Gene Name Synonym: N/A
Gi #: N/A	NCBI Acc #: NP_005441.1	Swiss Prot Acc #: Q13253
Length (aa): N/A	Mol. Weight (Da): 25774	Chrom Location: N/A

Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-His-Tyr-Leu

Biological Activity: The ED50, calculated by the ability to inhibit 5ng/ml of BMP-4 induced alkaline phosphatase production by ATCD-5 chondrogenic cells < 0.06 ug/ml.

Protein Content: Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.76 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of Noggin as a Reference Standard.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Solubility: It is recommended to reconstitute the lyophilized Noggin in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source/Host: E. Coli

Purity/Purification: Greater than 95.0% as determined by (a)Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Format: Lyophilized from a 0.2um filtered solution containing no additives.

Stability: Lyophilized Noggin although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution Noggin should be stored at 4 C between 2-7 days and for future use below -18 C.
For long term storage it is recommended to

Shipping: Products may be shipped on ice pack or dry ice.

TESTING: (secondary reagents and protocols )

Not Available

NOG PROTEIN TARGET DESCRIPTION:

Synonym Names for NOG protein: NOG; SYM1, SYNS1, NOG.; N/A; Noggin

Noggin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 206 amino acids and having a total molecular mass of 23147 Dalton.
Noggin is purified by proprietary chromatographic techniques.

The secreted polypeptide noggin, encoded by the NOG gene, binds and inactivates members of the transforming growth factor-beta (TGF-beta) superfamily signaling proteins, such as bone morphogenetic protein-4 (BMP4). By diffusing through extracellular matrices more efficiently than members of the TGF-beta superfamily, noggin may have a principal role in creating morphogenic gradients. Noggin appears to have pleiotropic effect, both early in development as well as in later stages. It was originally isolated from Xenopus based on its ability to restore normal dorsal-ventral body axis in embryos that had been artificially ventralized by UV treatment. The results of the mouse knockout of noggin suggest that it is involved in numerous developmental processes, such as neural tube fusion and joint formation. Recently, several dominant human NOG mutations in unrelated families with proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) were identified; both SYM1 and SYNS1 have multiple joint fusion as their principal feature, and map to the same region (17q22) as NOG. All NOG mutations altered evolutionarily conserved amino acid residues. The amino acid sequence of human noggin is highly homologous to that of Xenopus, rat and mouse.

Function: Essential for cartilage morphogenesis and joint formation. Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite (By similarity).

Subunit: Homodimer; disulfide-linked (By similarity).

Subcellular Location: Secreted.

Disease: Defects in NOG are a cause of symphalangism proximal syndrome (SYM1) [MIM:185800]. SYM1 is characterized by the hereditary absence of the proximal interphalangeal (PIP) joints (Cushing symphalangism). Severity of PIP joint involvement diminishes towards the radial side. Distal interphalangeal joints are less frequently involved and metacarpophalangeal joints are rarely affected whereas carpal bone malformation and fusion are common. In the lower extremities, tarsal bone coalition is common. Conducive hearing loss is seen and is due to fusion of the stapes to the petrous part of the temporal bone.

Disease: Defects in NOG are the cause of multiple synostoses syndrome 1 (SYNS1) [MIM:186500]; also known as synostoses, multiple, with brachydactyly/symphalangism-brachydactyly syndrome. SYNS1 is characterized by tubular-shaped (hemicylindrical) nose with lack of alar flare, otosclerotic deafness, and multiple progressive joint fusions commencing in the hand. The joint fusions are progressive, commencing in the fifth proximal interphalangeal joint in early childhood (or at birth in some individuals) and progressing in an ulnar-to-radial and proximal-to-distal direction. With increasing age, ankylosis of other joints, including the cervical vertebrae, hips, and humeroradial joints, develop.

Disease: Defects in NOG are the cause of tarsal-carpal coalition syndrome (TCC) [MIM:186570]. TCC is an autosomal dominant disorder characterized by fusion of the carpals, tarsals and phalanges, short first metacarpals causing brachydactyly, and humeroradial fusion. TCC is allelic to SYM1, and different mutations in NOG can result in either TCC or SYM1 in different families.

Disease: Defects in NOG are a cause of stapes ankylosis with broad thumb and toes [MIM:184460]. Stapes ankylosis with broad thumb and toes is a congenital autosomal dominant disorder that includes hyperopia, a hemicylindrical nose, broad thumbs, great toes, and other minor skeletal anomalies but lacked carpal and tarsal fusion and symphalangism.

Disease: Defects in NOG are the cause of brachydactyly type B2 (BDB2) [MIM:611377]. BDB2 is a subtype of brachydactyly characterized by hypoplasia/aplasia of distal phalanges in combination with distal symphalangism, fusion of carpal/tarsal bones, and partial cutaneous syndactyly.

Similarity: Belongs to the noggin family.

OMIM: 184460; phenotype. [NCBI / EBI]
185800; phenotype. [NCBI / EBI]
186500; phenotype. [NCBI / EBI]
186570; phenotype. [NCBI / EBI]
602991; gene. [NCBI / EBI]
611377; phenotype. [NCBI / EBI]

Products similar to NOG protein:

IgG

Fibrinogen [49D2], IgG

IgM, IgG

kappa Light chain [OX-20], IgG

Bradykinin, IgG

Fibrinogen [40F11], IgG

PRODUCT INTERACTIONS:

Noggin Human interacts with BMP-7 Human, CHO, Protein (GenWay Catalog #: 10-663-45804).

Noggin Human interacts with BMP-7 Human, Protein (GenWay Catalog #: 10-663-45804).

Noggin Human interacts with BMP-7, Protein (GenWay Catalog #: 10-663-45804).

Noggin Human interacts with BMP-7, IgG (GenWay Catalog #: 10-663-45804).

Noggin Human interacts with BMP7 (clone 4E7), IgG (GenWay Catalog #: 10-663-45804).

Noggin Human interacts with BMP7 (bone morphogenetic protein 7 (osteogenic protein 1)), IgG (GenWay Catalog #: 10-663-45804).

SUGGESTED PROTEIN REAGENTS - GenWay offers reagents to facilitate your research with cell-based assays, Mass Spectrometry, standards, calibrators, Western blot, cell culture, antibiotics, microwell substrates, and more!

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: NOG protein is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of NOG protein vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about NOG protein, all related information, articles, resources about NOG protein are being stored on our online database. Let us know if you have questions regarding this product.

Disclaimer: For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed.

GenWay is a Protein and Antibody Solutions Provider. Customer Satisfaction is Our Top Priority.

GenWay has a diverse line of recombinant protein expression platforms, including E. coli expression, Bacliovirus expression, yeast expression, and mammalian expression. Combining the technologies of bioinformatics, protein domain analysis, gene codon optimization, protein fusion, and protein expression in different host cells, GenWay can produce soluble and functional proteins. In particular, GenWay has the capability and vector systems for expression of difficult proteins such as proteases, kinases, glycosylated and membrane proteins. We have a very high success rate (almost 100%) and can deliver the proteins in a timely fashion. This is a core competency necessary for efficient and effective production of immunogens and affinity ligands for antibody production and purification. GenWay has over 300

recombinant proteins

offered in our catalog.



GenWay Biotech, Inc. 6777 Nancy Ridge Drive, San Diego, CA 92121 Tel: (858)458-0866 Fax: (858)458-0833 Email: sales@genwaybio.com www.genwaybio.com ©1998 - 2010 GenWay Biotech, Inc. - All Rights Reserved