SPOP protein, Speckle-type POZ protein recombinant protein

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Speckle-type POZ protein recombinant protein

Catalog Number: 10-288-21996F

Buy SPOP protein -

Size:

Related Product Names:

- SPOP protein; SPOP; TEF2; Speckle-type POZ protein
- Speckle-type POZ protein

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: SPOP	Gene Name Synonym: TEF2
Gi #: 4507183	NCBI Acc #: NP_003554.1	Swiss Prot Acc #: O43791
Length (aa): 374	Mol. Weight (Da): 42132	Chrom Location: 17q21.33

Linear Protein Map of Expressed Protein (sequence source from above GI#)

Fusion: T7 tag at N-terminus.

Source/Host: E Coli

Purity/Purification: 95%

Crossreactivity: human

Format: 10 mM Tris, pH 8.0, 0.1% Triton X-100, 0.002% NaN3.

Storage: -70 degree C. Avoid frequent freeze and thaw.

Stability: 6-12 months at -70 degree C.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for SPOP PROTEIN:

MS, SDS: Tested

ELISA, Western Blot: Not Tested

TESTING: (secondary reagents and protocols )

SDS: Analysis of SPOP Recombinant Protein. 4-20% SDS gradient gel. Coomassie blue staining. ( anti-SPOP protein )

SPOP protein SDS image
SPOP

SPOP PROTEIN TARGET DESCRIPTION:

Synonym Names for SPOP protein: SPOP; TEF2; Speckle-type POZ protein

Function: Inhibits IPF1/PDX1 transactivation of established target promoters, such as insulin, may be by recruiting a repressor complex (By similarity). In complex with CUL3, involved in ubiquitination of both PCGF4 and H2AFY.

Pathway: Ubiquitin conjugation; third step.

Subunit: Part of a complex consisting of PCGF4, CUL3 and SPOP. Part of a complex consisting of H2AFY, CUL3 and SPOP. Interacts with H2AFY, IPF1/PDX1 and PCGF4.

Subcellular Location: Nucleus.

Tissue Specificity: Widely expressed.

Domain: The MATH domain interacts with H2AFY and PCGF4.

Miscellaneous: Antigen recognized by serum from scleroderma patient.

Similarity: Belongs to the Tdpoz family.

Similarity: Contains 1 BTB (POZ) domain.

Similarity: Contains 1 MATH domain.

Summary: This protein is a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein.

Speckle-type POZ protein reacts with human.

OMIM: 602650; gene. [NCBI / EBI]

SUGGESTED PROTEIN REAGENTS - GenWay offers reagents to facilitate your research with cell-based assays, Mass Spectrometry, standards, calibrators, Western blot, cell culture, antibiotics, microwell substrates, and more!

BACKGROUND REFERENCES for SPOP PROTEIN:

Background references for antibody target are not specific to GenWay products

[1] Kwon,J.E., La,M., Oh,K.H., Oh,Y.M., Kim,G.R., Seol,J.H., Baek,S.H., Chiba,T., Tanaka,K., Bang,O.S., Joe,C.O. and Chung,C.H.
     BTB domain-containing speckle-type POZ protein (SPOP) serves as an adaptor of Daxx for ubiquitination by Cul3-based ubiquitin ligase
[2] Hernandez-Munoz,I., Lund,A.H., van der Stoop,P., Boutsma,E., Muijrers,I., Verhoeven,E., Nusinow,D.A., Panning,B., Marahrens,Y., et al.
     Stable X chromosome inactivation involves the PRC1 Polycomb complex and requires histone MACROH2A1 and the CULLIN3/SPOP ubiquitin E3 ligase
[3] La,M., Kim,K., Park,J., Won,J., Lee,J.H., Fu,Y.M., Meadows,G.G. and Joe,C.O.
     Daxx-mediated transcriptional repression of MMP1 gene is reversed by SPOP
[4] Zapata,J.M., Pawlowski,K., Haas,E., Ware,C.F., Godzik,A. and Reed,J.C.
     A diverse family of proteins containing tumor necrosis factor receptor-associated factor domains
[5] Nagai,Y., Kojima,T., Muro,Y., Hachiya,T., Nishizawa,Y., Wakabayashi,T. and Hagiwara,M.
     Identification of a novel nuclear speckle-type protein, SPOP
[6] , Nagai Y., Kojima T., Muro Y., Hachiya T., Nishizawa Y., Wakabayashi T., Hagiwara M.
     Identification of a novel nuclear speckle-type protein, SPOP.
[7] , Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., et al.
     Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
[8] , Hernandez-Munoz I., Lund A.H., van der Stoop P., Boutsma E., Muijrers I., Verhoeven E., Nusinow D.A., Panning B., Marahrens Y., van Lohuizen M., et al.
     Stable X chromosome inactivation involves the PRC1 Polycomb complex and requires histone MACROH2A1 and the CULLIN3/SPOP ubiquitin E3 ligase.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: SPOP protein is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of SPOP protein vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about SPOP protein, all related information, articles, resources about SPOP protein are being stored on our online database. Let us know if you have questions regarding this product.

Disclaimer: For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed.

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