SPARC protein, SPARC recombinant protein

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SPARC recombinant protein

Catalog Number: 10-288-22268F

Buy SPARC protein -

Size:

Related Product Names:

- SPARC protein; SPARC; ON; SPARC
- Secreted protein acidic and rich in cysteine; Osteonectin; ON; Basement-membrane protein 40; BM-40; Secreted protein, acidic, cysteine-rich (osteonectin)

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: SPARC	Gene Name Synonym: ON
Gi #: 4507171	NCBI Acc #: NP_003109.1	Swiss Prot Acc #: P09486
Length (aa): 303	Mol. Weight (Da): 34632	Chrom Location: 5q31.3-q32

Linear Protein Map of Expressed Protein (sequence source from above GI#)

Fusion: T7 tag at N-terminus.

Source/Host: E Coli

Purity/Purification: 95%

Crossreactivity: human

Format: 10 mM Tris, pH 8.0, 0.1% Triton X-100, 0.002% NaN3.

Storage: -70 degree C. Avoid frequent freeze and thaw.

Stability: 6-12 months at -70 degree C.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for SPARC PROTEIN:

MS, SDS: Tested

ELISA, Western Blot: Not Tested

TESTING: (secondary reagents and protocols )

SDS: Analysis of SPARC Recombinant Protein. 4-20% SDS gradient gel. Coomassie blue staining. ( anti-SPARC protein )

SPARC protein SDS image
SPARC

SPARC PROTEIN TARGET DESCRIPTION:

Synonym Names for SPARC protein: SPARC; ON; Secreted protein acidic and rich in cysteine; Osteonectin; ON; Basement-membrane protein 40; BM-40; Secreted protein, acidic, cysteine-rich (osteonectin)

Function: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.

Developmental Stage: Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.

Similarity: Belongs to the SPARC family.

Similarity: Contains 1 EF-hand domain.

Similarity: Contains 1 follistatin-like domain.

Similarity: Contains 1 Kazal-like domain.

Summary: Secreted protein acidic and rich in cysteine/osteonectin/BM40, or SPARC, is a matrix-associated protein that elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (ECM) (Bradshaw et al., 2003).[supplied by OMIM].

SPARC reacts with human.

OMIM: 182120; gene. [NCBI / EBI]

Pathways:
Reactome Event:Hemostasis 109582

Products similar to SPARC protein:

IgG

ICAM1 [15.2], IgG

ICAM1 [MEM-112], IgG

IL6 [MP5-20F3], IgG

Interferon gamma [H3-1], IgG

TRPM3 (transient receptor potential cation channel. subfamily M. member 3), IgG

SUGGESTED PROTEIN REAGENTS - GenWay offers reagents to facilitate your research with cell-based assays, Mass Spectrometry, standards, calibrators, Western blot, cell culture, antibiotics, microwell substrates, and more!

BACKGROUND REFERENCES for SPARC PROTEIN:

Background references for antibody target are not specific to GenWay products

[1] Kzhyshkowska,J., Workman,G., Cardo-Vila,M., Arap,W., Pasqualini,R., Gratchev,A., Krusell,L., Goerdt,S. and Sage,E.H.
     Novel function of alternatively activated macrophages: stabilin-1-mediated clearance of SPARC
[2] Campo McKnight,D.A., Sosnoski,D.M., Koblinski,J.E. and Gay,C.V., et al.
     Roles of osteonectin in the migration of breast cancer cells into bone
[3] Wang,H., Workman,G., Chen,S., Barker,T.H., Ratner,B.D., Sage,E.H. and Jiang,S.
     Secreted protein acidic and rich in cysteine (SPARC/osteonectin/BM-40) binds to fibrinogen fragments D and E, but not to native fibrinogen
[4] Delostrinos,C.F., Hudson,A.E., Feng,W.C., Kosman,J. and Bassuk,J.A., et al.
     The C-terminal Ca2+-binding domain of SPARC confers anti-spreading activity to human urothelial cells
[5] Ikuta,Y., Nakatsura,T., Kageshita,T., Fukushima,S., Ito,S., Wakamatsu,K., Baba,H. and Nishimura,Y.
     Highly sensitive detection of melanoma at an early stage based on the increased serum secreted protein acidic and rich in cysteine and glypican-3 levels
[6] , Lankat-Buttgereit B., Mann K., Deutzmann R., Timpl R., Krieg T.
     Cloning and complete amino acid sequences of human and murine basement membrane protein BM-40 (SPARC, osteonectin).
[7] , Swaroop A., Hogan B.L.M., Francke U.
     Molecular analysis of the cDNA for human SPARC/osteonectin/BM-40: sequence, expression, and localization of the gene to chromosome 5q31-q33.
[8] , Villarreal X.C., Mann K.G., Long G.L.
     Structure of human osteonectin based upon analysis of cDNA and genomic sequences.
[9] , Young M.F., Day A.A., Dominquez P., McQuillan C.I., Fisher L.W., Termine J.D.
     Structure and expression of osteonectin mRNA in human tissue.
[10] , Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., et al.
     Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: SPARC protein is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of SPARC protein vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about SPARC protein, all related information, articles, resources about SPARC protein are being stored on our online database. Let us know if you have questions regarding this product.

Disclaimer: For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed.

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