KLHL12 protein, Kelch-like protein 12 recombinant protein

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Peptides \| Secondary Antibodies \| ELISA Kits

Kelch-like protein 12 recombinant protein

Catalog Number: 10-288-22280F

Buy KLHL12 protein -

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Related Product Names:

- KLHL12 protein; KLHL12; C3IP1; DKIR; Kelch-like protein 12
- CUL3-interacting protein 1; Kelch-like 12

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: KLHL12	Gene Name Synonym: C3IP1; DKIR
Gi #: 11056006	NCBI Acc #: NP_067646.1	Swiss Prot Acc #: Q53G59
Length (aa): 568	Mol. Weight (Da): 63277	Chrom Location: 1q32.1

Linear Protein Map of Expressed Protein (sequence source from above GI#)

Fusion: T7 tag at N-terminus.

Source/Host: E Coli

Purity/Purification: 95%

Crossreactivity: human

Format: 10 mM Tris, pH 8.0, 0.1% Triton X-100, 0.002% NaN3.

Storage: -70 degree C. Avoid frequent freeze and thaw.

Stability: 6-12 months at -70 degree C.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for KLHL12 PROTEIN:

MS, SDS: Tested

ELISA, Western Blot: Not Tested

TESTING: (secondary reagents and protocols )

SDS: Analysis of KLHL12 Recombinant Protein. 4-20% SDS gradient gel. Coomassie blue staining. ( anti-KLHL12 protein )

KLHL12 protein SDS image
KLHL12

KLHL12 PROTEIN TARGET DESCRIPTION:

Synonym Names for KLHL12 protein: KLHL12; C3IP1; DKIR; CUL3-interacting protein 1; Kelch-like 12

Function: Serves as a substrate-specific adapter for the CUL3-based ubiquitin-protein E3 ligase complex. Negatively regulates the Wnt signaling pathway via the targeted ubiquitination and subsequent proteolysis of DVL3.

Subunit: Component of an ubiquitin-protein E3 ligase complex which includes at least CUL3 and KLHL12. This complex interacts with DVL3 upon activation of the Wnt signaling pathway by WNT3A.

Tissue Specificity: Highly expressed in testis and at lower levels in the submandibular salivary gland.

Domain: The BTB domain is required for interaction with CUL3.

Similarity: Contains 1 BTB (POZ) domain.

Similarity: Contains 6 Kelch repeats.

Kelch-like protein 12 reacts with human.

SUGGESTED PROTEIN REAGENTS - GenWay offers reagents to facilitate your research with cell-based assays, Mass Spectrometry, standards, calibrators, Western blot, cell culture, antibiotics, microwell substrates, and more!

BACKGROUND REFERENCES for KLHL12 PROTEIN:

Background references for antibody target are not specific to GenWay products

[1] Rual,J.F., Venkatesan,K., Hao,T., Hirozane-Kishikawa,T., Dricot,A., Li,N., Berriz,G.F., Gibbons,F.D., Dreze,M., Ayivi-Guedehoussou,N.,, et al.
     Towards a proteome-scale map of the human protein-protein interaction network
[2] Mai,A., Jung,S.K. and Yonehara,S., et al.
     hDKIR, a human homologue of the Drosophila kelch protein, involved in a ring-like structure
[3] Suzuki,Y., Yoshitomo-Nakagawa,K., Maruyama,K., Suyama,A. and Sugano,S.
     Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library
[4] Maruyama,K. and Sugano,S., et al.
     Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides
[5] , Du M., Zu Z., Liou J.-Y., Chu K.-Y., Sansores Garcia L., Yeh E., Wu K.K.
     Sequence of a novel kelch-like protein, C3IP1.
[6] , Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., et al.
     Complete sequencing and characterization of 21,243 full-length human cDNAs.
[7] , Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
     Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
[8] , Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., et al.
     Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
[9] , Uchida K., Akita Y., Matsuo K., Fujiwara S., Nakagawa A., Kazaoka Y., Hachiya H., Naganawa Y., Oh-Iwa I., Ohura K., et al.
     Identification of specific autoantigens in Sjoegren's syndrome by SEREX.
[10] , Angers S., Thorpe C.J., Biechele T.L., Goldenberg S.J., Zheng N., Maccoss M.J., Moon R.T.
     The KLHL12-cullin-3 ubiquitin ligase negatively regulates the Wnt-beta-catenin pathway by targeting Dishevelled for degradation.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: KLHL12 protein is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of KLHL12 protein vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about KLHL12 protein, all related information, articles, resources about KLHL12 protein are being stored on our online database. Let us know if you have questions regarding this product.

Disclaimer: For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed.

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