KHK protein, Ketohexokinase recombinant protein

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Peptides \| Secondary Antibodies \| ELISA Kits

Ketohexokinase recombinant protein

Catalog Number: 10-288-22373F

Buy KHK protein -

Size:

Related Product Names:

- KHK protein; KHK; Ketohexokinase
- EC 2.7.1.3; Hepatic fructokinase; Ketohexokinase isoform a

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: KHK	Gene Name Synonym: N/A
Gi #: 4557693	NCBI Acc #: NP_000212.1	Swiss Prot Acc #: P50053
Length (aa): 298	Mol. Weight (Da): 32730	Chrom Location: 2p23.3-p23.2

Linear Protein Map of Expressed Protein (sequence source from above GI#)

Fusion: T7 tag at N-terminus.

Source/Host: E Coli

Purity/Purification: 90%

Crossreactivity: human

Format: 10 mM Tris, pH 8.0, 0.1% Triton X-100, 0.002% NaN3.

Storage: -70 degree C. Avoid frequent freeze and thaw.

Stability: 6-12 months at -70 degree C.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for KHK PROTEIN:

MS, SDS: Tested

ELISA, Western Blot: Not Tested

TESTING: (secondary reagents and protocols )

SDS: Analysis of KHK Recombinant Protein. 4-20% SDS gradient gel. Coomassie blue staining. ( anti-KHK protein )

KHK protein SDS image
KHK

KHK PROTEIN TARGET DESCRIPTION:

Synonym Names for KHK protein: KHK; EC 2.7.1.3; Hepatic fructokinase; Ketohexokinase isoform a

Catalytic Activity: ATP + D-fructose = ADP + D-fructose 1-phosphate.

Enzyme Regulation: Requires potassium. Inhibition by ADP.

Pathway: Primary metabolism of dietary fructose in mammals.

Subunit: Dimer of A-A, A-C or C-C (Probable).

Tissue Specificity: Most abundant in liver, kidney, gut, spleen and pancreas. Low levels also found in adrenal, muscle, brain and eye.

Disease: Defects in KHK are the cause of fructosuria [MIM:229800]. Fructosuria is a benign defect of intermediary metabolism.

Similarity: Belongs to the carbohydrate kinase pfkB family.

Summary: KHK encodes the gene ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The splice variant presented encodes the highly active form found in liver, renal cortex, and small intestine, while the alternate variant encodes the lower activity form found in most other tissues.

Ketohexokinase reacts with human.

OMIM: 229800; gene+phenotype. [NCBI / EBI]

Pathways:
KEGG pathway: Fructose and mannose metabolism 00051
Reactome Event:Metabolism of sugars 71387

SUGGESTED PROTEIN REAGENTS - GenWay offers reagents to facilitate your research with cell-based assays, Mass Spectrometry, standards, calibrators, Western blot, cell culture, antibiotics, microwell substrates, and more!

BACKGROUND REFERENCES for KHK PROTEIN:

Background references for antibody target are not specific to GenWay products

[1] Hwa,J.S., Kim,H.J., Goo,B.M., Park,H.J., Kim,C.W., Chung,K.H., Park,H.C., Chang,S.H., Kim,Y.W., Kim,D.R., Cho,G.J., Choi,W.S. and, et al.
     The expression of ketohexokinase is diminished in human clear cell type of renal cell carcinoma
[2] Asipu,A., Hayward,B.E., O'Reilly,J. and Bonthron,D.T., et al.
     Properties of normal and mutant recombinant human ketohexokinases and implications for the pathogenesis of essential fructosuria
[3] Hayward,B.E. and Bonthron,D.T., et al.
     Structure and alternative splicing of the ketohexokinase gene
[4] Suzuki,Y., Yoshitomo-Nakagawa,K., Maruyama,K., Suyama,A. and Sugano,S.
     Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library
[5] Hayward,B.E., Fantes,J.A., Warner,J.P., Intody,S., Leek,J.P., Markham,A.F. and Bonthron,D.T.
     Co-localization of the ketohexokinase and glucokinase regulator genes to a 500-kb region of chromosome 2p23
[6] , Bonthron D.T., Brady N., Donaldson I.A., Steinmann B.
     Molecular basis of essential fructosuria: molecular cloning and mutational analysis of human ketohexokinase (fructokinase).
[7] , Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., et al.
     Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
[8] , Hayward B.E., Bonthron D.T.
     Structure and alternative splicing of the ketohexokinase gene.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: KHK protein is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of KHK protein vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about KHK protein, all related information, articles, resources about KHK protein are being stored on our online database. Let us know if you have questions regarding this product.

Disclaimer: For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed.

SERVICES AVAILABLE for KHK PROTEIN:

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