GenWay Log In My Account My Cart
Home Products Services Technologies QC & QA Support Company News & Events
Alphabetical Listing of Primary Antibodies: f| 0-4 | 5-9 | A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z |
Alphabetical Listing of Proteins: f| 0-4 | 5-9 | A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z |
Peptides  |  Secondary Antibodies  |  ELISA Kits
Growth factor receptor-bound protein 2 recombinant protein Printer Friendly Datasheet
Catalog Number: 10-288-22538F
Buy GRB2 protein - Size:   
  Related Product Names:
- GRB2 protein; GRB2; ASH; Grb3-3; MST084; MSTP084; Growth factor receptor-bound protein 2
- Adapter protein GRB2; SH2/SH3 adapter GRB2; Protein Ash; Growth factor receptor-bound protein 2 isoform 1
- Gene Information -
Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.
 Gene Name: GRB2  Gene Name Synonym: ASH; Grb3-3; MST084; MSTP084

 Gi #: 4504111
 NCBI Acc #: NP_002077.1
 Swiss Prot Acc #: P62993

 Length (aa): 217
 Mol. Weight (Da): 25206
 Chrom Location: 17q24-q25
GRB2
Linear Protein Map of Expressed Protein (sequence source from above GI#)
 Fusion: His-tag at N-terminus.

 Source/Host: E Coli

 Purity/Purification: 95%

 Crossreactivity: human

 Format: 10 mM Tris, pH 8.0, 0.002% NaN3, 3mM NaCl, 2.5mM Imidazole.

 Storage: -70 degree C. Avoid frequent freeze and thaw.

 Stability: 6-12 months at -70 degree C.

 Shipping: Products may be shipped on ice pack or dry ice.
 APPLICATIONS for GRB2 PROTEIN:
 MS, SDS: Tested
 ELISA, Western Blot: Not Tested
 TESTING: (secondary reagents and protocols )
 SDS: Analysis of GRB2 Recombinant Protein. 4-20% SDS gradient gel. Coomassie blue staining. ( anti-GRB2 protein )

GRB2 protein SDS image
GRB2
 GRB2 PROTEIN TARGET DESCRIPTION:
Synonym Names for GRB2 protein: GRB2; ASH; Grb3-3; MST084; MSTP084; Adapter protein GRB2; SH2/SH3 adapter GRB2; Protein Ash; Growth factor receptor-bound protein 2 isoform 1

Function: Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.

Function: Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.

Subunit: Associates with activated Tyr-phosphorylated EGF receptors and PDGF receptors via its SH2 domain. Also associates to other cellular Tyr-phosphorylated proteins such as SIT1, IRS1, SHC and LNK; probably via the concerted action of both its SH2 and SH3 domains. It also seems to interact with RAS in the signaling pathway leading to DNA synthesis. Binds to and translocates the guanine nucleotide exchange factors SOS. Interacts with phosphorylated TOM1L1 and MET. Interacts with the phosphorylated C-terminus of APS. Interacts with phosphorylated SIT1, LAX1, LAT, LAT2 and LIME1 upon TCR and/or BCR activation. Interacts with PTPNS1, REPS2 and the syntrophin SNTA1. Interacts with REPS1 and PIK3C2B via its SH3 domains (By similarity). Interacts with HCV NS5A via its SH3 domains. Interacts with CBL and CBLB.

Similarity: Belongs to the GRB2/sem-5/DRK family.

Similarity: Contains 1 SH2 domain.

Similarity: Contains 2 SH3 domains.

Summary: The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This protein is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Growth factor receptor-bound protein 2 reacts with human.

OMIM: 108355; gene. [NCBI / EBI]

Pathways:
KEGG pathway: Colorectal cancer 05210
KEGG pathway: Dorso-ventral axis formation 04320
KEGG pathway: Fc epsilon RI signaling pathway 04664
KEGG pathway: Focal adhesion 04510
KEGG pathway: Gap junction 04540
KEGG pathway: Glioma 05214
KEGG pathway: GnRH signaling pathway 04912
KEGG pathway: Huntington's disease 05040
KEGG pathway: Insulin signaling pathway 04910
KEGG pathway: Jak-STAT signaling pathway 04630
KEGG pathway: MAPK signaling pathway 04010
KEGG pathway: Natural killer cell mediated cytotoxicity 04650
KEGG pathway: Neurodegenerative Disorders 01510
KEGG pathway: T cell receptor signaling pathway 04660
Reactome Event:Insulin receptor mediated signaling 74752

Products similar to GRB2 protein:
 IgG
    Grb2, IgG
    ASCL2, IgG
    CASP8 associated protein 2, IgG
    CASP8 and FADD-like apoptosis regulator, IgG
    STS2 / TULA (N Terminus), IgG
 Native_Protein
    L-Asparaginase, Native_Protein
    PLA2 IID Human, Protein
 PRODUCT INTERACTIONS:
 Growth factor receptor-bound protein 2 interacts with HCV NS4 Mosaic Genotype-2, Protein (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with HCV NS4 Mosaic Genotype-2, Protein (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with HCV NS5 Genotype-2a, Protein (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with HCV NS5 Genotype-2, Protein (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with HCV Core Genotype-2a, Protein (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with HCV Core Ag,genotype 2,, Protein (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with Dynamin [D5], IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with Dynamin [0.T.178], IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with Dynamin 1 [4E67], IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGF R, IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR (ready-to-use), IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR [ICR10], IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR [Cocktail R19/48 (111.6 + 199.12 + F4 + 11E8)], IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR [199.12], IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR [2E9], IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with Acitve EGF Receptor EIA Kit, ELISA_Kits (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR (Phospho-Ser1070), IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR (Phospho-Tyr1092), IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR (Phospho-Thr678), IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR (Phospho-Thr693), IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR (Phospho-Tyr1172), IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR (Phospho-Tyr1197), IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR (Phospho-Tyr869), IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR (phospho-Tyr1110), IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR (Ab-1070), IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR (Ab-1092), IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR (Ab-678), IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR (Ab-693), IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR (Ab-1172), IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR (Ab-1197), IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR (Ab-869), IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR (Ab-1110), IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR (Phospho-Ser1070) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR (Phospho-Tyr1092) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR(Phospho-Tyr1172) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR (Phospho-Tyr1197) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR (Phospho-Tyr869) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR (phospho-Tyr1110) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR (External Domain), IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR (Internal Domain), IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR pTyr 845, IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR (a.a. 1165-1186), IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR dpTyr 1173, IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR, Activated pTyr 1173, IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR pS1047, IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR pThr 654, IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR, pTyr 1045, IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGF Receptor (non-ligand binding site), IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGF Receptor (ligand binding site), IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGF Receptor, IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGF Receptor, IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with Rabbit anti EGFR (a.a. 1168-1181), IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with Rabbit anti EGF Receptor, IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR Human Sf9, Protein (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR Human, Protein (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR Human Sf9, Protein (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with Anti-Epidermal Growth Factor Receptor (EGFR) pY1197, IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with Anti-Epidermal Growth Factor Receptor (EGFR) pY1197, IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR [ICR10] (FITC), IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR [29.1], IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR, IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR phospho Y1148, IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR [SP9] (ready-to-use), IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR [EGFR1], IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGF R, IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR phospho Y1173, IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR phospho Y1068, IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR phospho Y845, IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR [F4], IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR [ICR61], IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR [R19/48], IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR [H9B4], IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR [F4], IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR phospho Y1086, IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR phospho Y992, IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGFR [F4] (FITC), IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with EGF R, IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with epidermal growth factor receptor isoform a precursor, IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with epidermal growth factor receptor isoform a precursor, IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with epidermal growth factor receptor isoform a precursor, IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with KH domain-containing, RNA-binding, signal transduction-associated protein 1, IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with VAV1 phospho Y160, IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with Vav (Phospho-Tyr174), IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with Vav (Ab-174), IgG (GenWay Catalog #: 10-288-22538F).
 Growth factor receptor-bound protein 2 interacts with Vav (Phospho-Tyr174) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 10-288-22538F).
SUGGESTED PROTEIN REAGENTS - GenWay offers reagents to facilitate your research with cell-based assays, Mass Spectrometry, standards, calibrators, Western blot, cell culture, antibiotics, microwell substrates, and more!
 BACKGROUND REFERENCES for GRB2 PROTEIN:
Background references for antibody target are not specific to GenWay products
[1] Di Fulvio,M., Lehman,N., Lin,X., Lopez,I. and Gomez-Cambronero,J., et al.
     The elucidation of novel SH2 binding sites on PLD2
[2] Johannessen,L.E., Pedersen,N.M., Pedersen,K.W., Madshus,I.H. and Stang,E.
     Activation of the epidermal growth factor (EGF) receptor induces formation of EGF receptor- and Grb2-containing clathrin-coated pits
[3] Choi,J.H., Hong,W.P., Yun,S., Kim,H.S., Lee,J.R., Park,J.B., Bae,Y.S., Ryu,S.H. and Suh,P.G.
     Grb2 negatively regulates epidermal growth factor-induced phospholipase C-gamma1 activity through the direct interaction with tyrosine-phosphorylated phospholipase C-gamma1
[4] Martin,M., Del Valle,J.M., Saborit,I. and Engel,P., et al.
     Identification of Grb2 as a novel binding partner of the signaling lymphocytic activation molecule-associated protein binding receptor CD229
[5] Patrussi,L., Savino,M.T., Pellegrini,M., Paccani,S.R., Migliaccio,E., Plyte,S., Lanfrancone,L., Pelicci,P.G. and, et al.
     Cooperation and selectivity of the two Grb2 binding sites of p52Shc in T-cell antigen receptor signaling to Ras family GTPases and Myc-dependent survival
[6] , Lowenstein E.J., Daly R.J., Batzer A.G., Li W., Margolis B., Lammers R., Ullrich A., Skolnik E.Y., Bar-Sagi D., Schlessinger J., et al.
     The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling.
[7] , Matuoka K., Yamakawa A., Shibata M., Takenawa T.
     Cloning of ASH, a ubiquitous protein composed of one Src homology region (SH) 2 and two SH3 domains, from human and rat cDNA libraries.
[8] , Fath I., Schweighoffer F., Rey I., Multon M.-C., Boiziau J., Duchesne M., Tocque B.
     Cloning of a Grb2 isoform with apoptotic properties.
[9] , Bochmann H., Gehrisch S., Jaross W.
     The gene structure of the human growth factor bound protein GRB2.
[10] , Puhl H.L. III, Ikeda S.R., Aronstam R.S.
     cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org).
Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: GRB2 protein is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of GRB2 protein vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about GRB2 protein, all related information, articles, resources about GRB2 protein are being stored on our online database. Let us know if you have questions regarding this product.

Disclaimer: For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed.
 SERVICES AVAILABLE for GRB2 PROTEIN:
 Are you interested in ELISA development for this protein?
 ELISA kit and other assay development services.
 Protein Expression Services for GRB2
 Function protein expression services.
 For more information about the antigen please send us a question.
 Having questions about IgY? Visit our IgY Resource and FAQs page.
 Please inquire about antigen, protein availability or custom chicken igy antibody services.

GenWay is a Protein and Antibody Solutions Provider. Customer Satisfaction is Our Top Priority.
GenWay has a diverse line of recombinant protein expression platforms, including E. coli expression, Bacliovirus expression, yeast expression, and mammalian expression. Combining the technologies of bioinformatics, protein domain analysis, gene codon optimization, protein fusion, and protein expression in different host cells, GenWay can produce soluble and functional proteins. In particular, GenWay has the capability and vector systems for expression of difficult proteins such as proteases, kinases, glycosylated and membrane proteins. We have a very high success rate (almost 100%) and can deliver the proteins in a timely fashion. This is a core competency necessary for efficient and effective production of immunogens and affinity ligands for antibody production and purification. GenWay has over 300

recombinant proteins

offered in our catalog.
Exact   |  Advanced Search

Select by Product Name
Prod Name: 
Products Type: 
IgG IgY
Protein Peptide
Applications: 
Source: 
Xreactivity: 
Conjugate: 
 
Specific Categories
Antibodies
Secondary Antibodies
Proteins
Custom Antibody
ELISA Kits
Reagents
Products by Pathway
Protein Expression
Cell Line Development
 
 
 
GenWay Biotech, Inc.  6777 Nancy Ridge Drive, San Diego, CA 92121
Tel: (858)458-0866  Fax: (858)458-0833  Email: sales@genwaybio.com
www.genwaybio.com ©1998 - 2010 GenWay Biotech, Inc. - All Rights Reserved