RB1 antibody, retinoblastoma 1 (including osteosarcoma) (RB1) Antibody, Mouse Monoclonal Antibody

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Peptides \| Secondary Antibodies \| ELISA Kits

retinoblastoma 1 (including osteosarcoma) (RB1) Antibody, Mouse Monoclonal Antibody

Catalog Number: 20-372-60054

Buy RB1 antibody -

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Related Product Names:

- RB1 antibody; RB1; RB; OSRC; retinoblastoma 1 (including osteosarcoma) (RB1)
- Mouse monoclonal anti-human RB1 antibody; PP110; P105-RB; RB; Retinoblastoma 1; Retinoblastoma-associated protein

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: RB1	Gene Name Synonym: RB; OSRC
Gi #: 108773787	NCBI Acc #: NP_000312.2	Swiss Prot Acc #: P06400
Length (aa): 928	Mol. Weight (Da): 106159	Chrom Location: N/A

Clone: RB1AD56A

IGG Subclass: IgG1

Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.

Sterility: Filtered through a 0.22 um membrane.

Source/Host: Mouse

Clonality: Monoclonal

Crossreactivity: Human

Format: Mouse monoclonal anti-human RB1 antibody in PBS (3.0 mM KCl, 1.5 mM K2HPO4, 140 mM NaCl, 8.0 mM Na2HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3)

Storage: Store at 2-8 C for up to one year. We recommend storing at –20 C for long-term storage. Avoid repeat freezing and thawing cycles.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for RB1 ANTIBODY:

DB, ELISA: Tested

TESTING: (secondary reagents and protocols )

Western Blot ( anti-RB1 )

RB1 antibody tern B image
RB1

RB1 ANTIBODY TARGET DESCRIPTION:

Synonym Names for RB1 antibody: RB1; RB; OSRC; Mouse monoclonal anti-human RB1 antibody; PP110; P105-RB; RB; Retinoblastoma 1; Retinoblastoma-associated protein

Function: Key regulator of entry into cell division that acts as a tumor suppressor. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Also acts as a transcription repressor of E2F target genes by recruiting chromatin-modifying enzymes to promoters. Inhibits the intrinsic kinase activity of TAF1. Forms a complex with adenovirus E1A and with SV40 large T antigen. May bind and modulate functionally certain cellular proteins with which T and E1A compete for pocket binding.

Subunit: Interacts preferentially with transcription factor E2F1. The unphosphorylated form interacts with ARID3B, JARID1A, SUV39H1, MJD2A/JHDM3A and THOC1. Interacts with the N-terminal domain of TAF1. Interacts with AATF, DNMT1, LIN9, LMNA, SUV420H1, SUV420H2, PELP1 and TMPO-alpha. May interact with KNTC2. Interacts with EID1 and ZUBR1. Interacts with ARID4A and JARID1B.

Subcellular Location: Nucleus.

Tissue Specificity: Expressed in the retina.

Ptm: Phosphorylated from S to M phase of the cell cycle and is dephosphorylated in G1. T, but not E1A, binds only to the unphosphorylated form.

Disease: Defects in RB1 are the cause of childhood cancer retinoblastoma (RB) [MIM:180200]. RB is a congenital malignant tumor that arises from the nuclear layers of the retina. It occurs in about 1:20'000 live births and represents about 2% of childhood malignancies. It is bilateral in about 30% of cases. Although most RB appear sporadically, about 20% are transmitted as an autosomal dominant trait with incomplete penetrance. The diagnosis is usually made before the age of 2 years when strabismus or a gray to yellow reflex from pupil ("cat eye") is investigated.

Disease: Defects in RB1 are a cause of bladder cancer [MIM:109800].

Disease: Defects in RB1 are a cause of osteogenic sarcoma [MIM:259500].

Similarity: Belongs to the retinoblastoma protein (RB) family.

Summary: Retinoblastoma (RB) is an embryonic malignant neoplasm of retinal origin. It almost always presents in early childhood and is often bilateral. Spontaneous regression ('cure') occurs in some cases.[supplied by OMIM].

retinoblastoma 1 (including osteosarcoma) (RB1) reacts with human.

OMIM: 109800; phenotype. [NCBI / EBI]
180200; gene+phenotype. [NCBI / EBI]
259500; phenotype. [NCBI / EBI]

Pathways:
KEGG pathway: Cell cycle 04110
KEGG pathway: Chronic myeloid leukemia 05220
KEGG pathway: Glioma 05214
KEGG pathway: Melanoma 05218
KEGG pathway: Non-small cell lung cancer 05223
KEGG pathway: Pancreatic cancer 05212
KEGG pathway: Prostate cancer 05215
KEGG pathway: Small cell lung cancer 05222
Reactome Event:Cell Cycle (Mitotic) 69278
Reactome Event:DNA Replication 69306

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PRODUCT INTERACTIONS:

retinoblastoma 1 (including osteosarcoma) (RB1) interacts with CDK2, IgG (GenWay Catalog #: 20-372-60054).

retinoblastoma 1 (including osteosarcoma) (RB1) interacts with Cdk2, IgG (GenWay Catalog #: 20-372-60054).

retinoblastoma 1 (including osteosarcoma) (RB1) interacts with CDK2 (Phospho-Thr160), IgG (GenWay Catalog #: 20-372-60054).

retinoblastoma 1 (including osteosarcoma) (RB1) interacts with CDK2 (Ab-160), IgG (GenWay Catalog #: 20-372-60054).

retinoblastoma 1 (including osteosarcoma) (RB1) interacts with CDK2 (Phospho-Thr160) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 20-372-60054).

retinoblastoma 1 (including osteosarcoma) (RB1) interacts with CDK2, IgG (GenWay Catalog #: 20-372-60054).

retinoblastoma 1 (including osteosarcoma) (RB1) interacts with Anti-cdk2, IgG (GenWay Catalog #: 20-372-60054).

retinoblastoma 1 (including osteosarcoma) (RB1) interacts with E2F1 (E2F transcription factor 1), IgG (GenWay Catalog #: 20-372-60054).

retinoblastoma 1 (including osteosarcoma) (RB1) interacts with E2F1 [KH129], IgG (GenWay Catalog #: 20-372-60054).

retinoblastoma 1 (including osteosarcoma) (RB1) interacts with E2F1 [6B5], IgG (GenWay Catalog #: 20-372-60054).

retinoblastoma 1 (including osteosarcoma) (RB1) interacts with E2F1 [18E10], IgG (GenWay Catalog #: 20-372-60054).

retinoblastoma 1 (including osteosarcoma) (RB1) interacts with E2F1 [10D1 2B5], IgG (GenWay Catalog #: 20-372-60054).

retinoblastoma 1 (including osteosarcoma) (RB1) interacts with E2F1 [17E2], IgG (GenWay Catalog #: 20-372-60054).

retinoblastoma 1 (including osteosarcoma) (RB1) interacts with E2F1 (E2F transcription factor 1), IgG (GenWay Catalog #: 20-372-60054).

retinoblastoma 1 (including osteosarcoma) (RB1) interacts with Anti-E2F-1 pS364 (Rabbit), IgG (GenWay Catalog #: 20-372-60054).

retinoblastoma 1 (including osteosarcoma) (RB1) interacts with E2F1, IgG (GenWay Catalog #: 20-372-60054).

retinoblastoma 1 (including osteosarcoma) (RB1) interacts with E2F1 [18E10], IgG (GenWay Catalog #: 20-372-60054).

retinoblastoma 1 (including osteosarcoma) (RB1) interacts with E2F1 [16G7], IgG (GenWay Catalog #: 20-372-60054).

retinoblastoma 1 (including osteosarcoma) (RB1) interacts with E2F1 [10D1 2B5], IgG (GenWay Catalog #: 20-372-60054).

retinoblastoma 1 (including osteosarcoma) (RB1) interacts with E2F1 [17E2], IgG (GenWay Catalog #: 20-372-60054).

retinoblastoma 1 (including osteosarcoma) (RB1) interacts with HDAC1 (histone deacetylase 1), IgG (GenWay Catalog #: 20-372-60054).

retinoblastoma 1 (including osteosarcoma) (RB1) interacts with HDAC1 [HDAC1-21], IgG (GenWay Catalog #: 20-372-60054).

retinoblastoma 1 (including osteosarcoma) (RB1) interacts with Anti-HDAC-1, IgG (GenWay Catalog #: 20-372-60054).

retinoblastoma 1 (including osteosarcoma) (RB1) interacts with HDAC1, IgG (GenWay Catalog #: 20-372-60054).

retinoblastoma 1 (including osteosarcoma) (RB1) interacts with HDAC1 (ready-to-use), IgG (GenWay Catalog #: 20-372-60054).

retinoblastoma 1 (including osteosarcoma) (RB1) interacts with HDAC1, IgG (GenWay Catalog #: 20-372-60054).

retinoblastoma 1 (including osteosarcoma) (RB1) interacts with PRMT2 (protein arginine methyltransferase 2), IgG (GenWay Catalog #: 20-372-60054).

retinoblastoma 1 (including osteosarcoma) (RB1) interacts with MORF4L2 (mortality factor 4 like 2), IgG (GenWay Catalog #: 20-372-60054).

retinoblastoma 1 (including osteosarcoma) (RB1) interacts with TBP Associated Factor 1 [0.T.145], IgG (GenWay Catalog #: 20-372-60054).

retinoblastoma 1 (including osteosarcoma) (RB1) interacts with TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa), IgG (GenWay Catalog #: 20-372-60054).

retinoblastoma 1 (including osteosarcoma) (RB1) interacts with Anti-Transcription Initiation Factor TFIID Subunit 1 (TAF1), IgG (GenWay Catalog #: 20-372-60054).

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

BACKGROUND REFERENCES for RB1 ANTIBODY:

Background references for antibody target are not specific to GenWay products

[1] Rastetter,M., Schagdarsurengin,U., Lahtz,C., Fiedler,E., Marsch,W.Ch., Dammann,R. and Helmbold,P.
     Frequent intra-tumoural heterogeneity of promoter hypermethylation in malignant melanoma
[2] Ding,L., Li,L., Yang,J., Zhou,S., Li,W., Tang,M., Shi,Y., Yi,W. and Cao,Y.
     Latent membrane protein 1 encoded by Epstein-Barr virus induces telomerase activity via p16INK4A/Rb/E2F1 and JNK signaling pathways
[3] Sharma,A., Comstock,C.E., Knudsen,E.S., Cao,K.H., Hess-Wilson,J.K., Morey,L.M., Barrera,J. and Knudsen,K.E.
     Retinoblastoma tumor suppressor status is a critical determinant of therapeutic response in prostate cancer cells
[4] Park,H.W., Song,S.Y., Lee,T.J., Jeong,D. and Lee,T.Y., et al.
     Abrogation of the p16-retinoblastoma-cyclin D1 pathway in head and neck squamous cell carcinomas
[5] Nemtsova,M.V., Zemliakova,V.V., Kuznetsova,E.V., Babenko,O.V., Zavalishina,L.E., Andreeva,Iu.Iu., Bateva,M.V., Frank,G.A. and, et al.
     [Analysis of the correlation between genetic and immunohistochemical markers (RB1, p16 and p53 gene regultors of a cell cycle) in sporadic mammary gland carcinoma]
[6] Lee W.-H., Shew J.-Y., Hong F.D., Sery T.W., Donoso L.A., Young L.-J.S., Bookstein R., Lee E.Y.-H.P.
     The retinoblastoma susceptibility gene encodes a nuclear phosphoprotein associated with DNA binding activity.
[7] Lee W.-H., Bookstein R., Hong F.D., Young L.-J., Shew J.-Y., Lee E.Y.-H.P.
     Human retinoblastoma susceptibility gene: cloning, identification, and sequence.
[8] Friend S.H., Horowitz J.M., Gerber M.R., Wang X.-F., Bogenmann E., Li F.P., Weinberg R.A.
     Deletions of a DNA sequence in retinoblastomas and mesenchymal tumors: organization of the sequence and its encoded protein.
[9] McGee T.L., Yandell D.W., Dryja T.P.
     Structure and partial genomic sequence of the human retinoblastoma susceptibility gene.
[10] Hogg A., Onadim Z., Baird P.N., Cowell J.K.
     Detection of heterozygous mutations in the RB1 gene in retinoblastoma patients using single-strand conformation polymorphism analysis and polymerase chain reaction sequencing.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: RB1 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of RB1 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about RB1 antibody, all related information, articles, resources about RB1 antibody are being stored on our online database. Let us know if you have questions regarding this product.

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