ELF3 antibody, E74-like factor 3 (ets domain transcription factor,epithelial-specific ) (ELF3) Antibody, Mouse Monoclonal Antibody

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E74-like factor 3 (ets domain transcription factor,epithelial-specific ) (ELF3) Antibody, Mouse Monoclonal Antibody

Catalog Number: 20-372-60074

Buy ELF3 antibody -

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Related Product Names:

- ELF3 antibody; ELF3; ERT; ESX; EPR-1; ESE-1; E74-like factor 3 (ets domain transcription factor,epithelial-specific ) (ELF3)
- Mouse monoclonal anti-human ELF3 antibody; E74-like factor 3; Epithelium-specific Ets transcription factor 1; ESE-1; Epithelium-restricted Ets protein ESX; Epithelial-restricted with serine box; E74-like factor 3 (ets domain transcription factor, epithelial-specific ); ETS-related transcription factor Elf-3

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: ELF3	Gene Name Synonym: ERT; ESX; EPR-1; ESE-1
Gi #: 40255035	NCBI Acc #: NP_004424.2	Swiss Prot Acc #: P78545
Length (aa): 371	Mol. Weight (Da): 41454	Chrom Location: N/A

Clone: ELF3B10C4

IGG Subclass: IgG1

Antibody concentration: 100µg/ml (1.0 ml)

Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.

Sterility: Filtered through a 0.22 um membrane.

Disposal: This antibody solution contains sodium azide (NaN₃) as a preservative. There is a potential hazard that NaN₃ reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water.

Source/Host: Mouse

Clonality: Monoclonal

Crossreactivity: Human

Format: Mouse monoclonal anti-human ELF3 antibody in PBS (3.0 mM KCl, 1.5 mM K2HPO4, 140 mM NaCl, 8.0 mM Na2HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3).

Storage: Store at 2-8 C for up to one year. We recommend storing at -20 C for long-term storage. Avoid repeat freezing and thawing cycles.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for ELF3 ANTIBODY:

DB: Tested

TESTING: (secondary reagents and protocols )

Western Blot ( anti-ELF3 )

ELF3 antibody tern B image
ELF3

ELF3 ANTIBODY TARGET DESCRIPTION:

Synonym Names for ELF3 antibody: ELF3; ERT; ESX; EPR-1; ESE-1; Mouse monoclonal anti-human ELF3 antibody; E74-like factor 3; Epithelium-specific Ets transcription factor 1; ESE-1; Epithelium-restricted Ets protein ESX; Epithelial-restricted with serine box; E74-like factor 3 (ets domain transcription factor, epithelial-specific ); ETS-related transcription factor Elf-3

Function: Transcriptional activator that binds and transactivates ETS sequences containing the consensus nucleotide core sequence GGA[AT]. Acts synergistically with POU2F3 to transactivate the SPRR2A promoter and with RUNX1 to transactivate the ANGPT1 promoter. Also transactivates collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2 and TGM3 promoters. Represses KRT4 promoter activity. Involved in mediating vascular inflammation. May play an important role in epithelial cell differentiation and tumorigenesis. May be a critical downstream effector of the ERBB2 signaling pathway. May be associated with mammary gland development and involution. Plays an important role in the regulation of transcription with TATA-less promoters in preimplantation embryos, which is essential in preimplantation development (By similarity).

Subunit: Interacts with TBP. Interacts with CREBBP and EP300; these act as transcriptional coactivators of ELF3 and positively modulate its function. Interacts with XRCC5/KU86 and XRCC6/KU70; these inhibit the ability of ELF3 to bind DNA and negatively modulate its transcriptional activity. Associated with CLND7 and POU2F3.

Subcellular Location: Cytoplasm. Nucleus. Note=Localizes to the cytoplasm where it has been shown to transform MCF-12A mammary epithelial cells via a novel cytoplasmic mechanism. Also transiently expressed and localized to the nucleus where it induces apoptosis in non-transformed breast epithelial cells MCF-10A and MCF-12A via a transcription-dependent mechanism.

Tissue Specificity: Expressed exclusively in tissues containing a high content of terminally differentiated epithelial cells including mammary gland, colon, trachea, kidney, prostate, uterus, stomach and skin.

Induction: Transcriptionally regulated by ERBB2 receptor signaling in breast cancer epithelial cells. Up-regulated by phorbol-12-myristate-13-acetate (PMA) in bronchial epithelial cells. By retinoic acid in MCF-7 mammary epithelial cells (at protein level).

Similarity: Belongs to the ETS family.

Similarity: Contains 1 ETS DNA-binding domain.

Similarity: Contains 1 SAM (sterile alpha motif) domain.

E74-like factor 3 (ets domain transcription factor,epithelial-specific ) (ELF3) reacts with human.

OMIM: 602191; gene. [NCBI / EBI]

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ELF3 (E74-like factor 3 (ets domain transcription factor. epithelial-specific )), IgG

Protein

Lipid-transfer protein CERTL, Protein

PRODUCT INTERACTIONS:

E74-like factor 3 (ets domain transcription factor,epithelial-specific ) (ELF3) interacts with Human Calreticulin, Protein (GenWay Catalog #: 20-372-60074).

E74-like factor 3 (ets domain transcription factor,epithelial-specific ) (ELF3) interacts with CALR (calreticulin), IgG (GenWay Catalog #: 20-372-60074).

E74-like factor 3 (ets domain transcription factor,epithelial-specific ) (ELF3) interacts with EHF (ets homologous factor), IgG (GenWay Catalog #: 20-372-60074).

E74-like factor 3 (ets domain transcription factor,epithelial-specific ) (ELF3) interacts with alpha Glucosidase II, IgG (GenWay Catalog #: 20-372-60074).

E74-like factor 3 (ets domain transcription factor,epithelial-specific ) (ELF3) interacts with HIV-1 p55 gag, Protein (GenWay Catalog #: 20-372-60074).

E74-like factor 3 (ets domain transcription factor,epithelial-specific ) (ELF3) interacts with PDI Human, Protein (GenWay Catalog #: 20-372-60074).

E74-like factor 3 (ets domain transcription factor,epithelial-specific ) (ELF3) interacts with PDI [PDI-38H8], IgG (GenWay Catalog #: 20-372-60074).

E74-like factor 3 (ets domain transcription factor,epithelial-specific ) (ELF3) interacts with PL-7 Human, Protein (GenWay Catalog #: 20-372-60074).

E74-like factor 3 (ets domain transcription factor,epithelial-specific ) (ELF3) interacts with threonyl-tRNA synthetase, mRNA (cDNA clone MGC:9344 IMAGE:3458845), complete cds, IgG (GenWay Catalog #: 20-372-60074).

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

BACKGROUND REFERENCES for ELF3 ANTIBODY:

Background references for antibody target are not specific to GenWay products

[1] Kopp,J.L., Wilder,P.J., Desler,M., Kinarsky,L. and Rizzino,A., et al.
     Different domains of the transcription factor ELF3 are required in a promoter-specific manner and multiple domains control its binding to DNA
[2] Ewing,R.M., Chu,P., Elisma,F., Li,H., Taylor,P., Climie,S., McBroom-Cerajewski,L., Robinson,M.D., O'Connor,L., Li,M.,, et al.
     Large-scale mapping of human protein-protein interactions by mass spectrometry
[3] Kohno,Y., Okamoto,T., Ishibe,T., Nagayama,S., Shima,Y., Nishijo,K., Shibata,K.R., Fukiage,K., Otsuka,S., Uejima,D., Araki,N., Naka,N.,, et al.
     Expression of claudin7 is tightly associated with epithelial structures in synovial sarcomas and regulated by an Ets family transcription factor, ELF3
[4] Wang,H., Fang,R., Cho,J.Y., Libermann,T.A. and Oettgen,P., et al.
     Positive and negative modulation of the transcriptional activity of the ETS factor ESE-1 through interaction with p300, CREB-binding protein, and Ku 70/86
[5] Prescott,J.D., Koto,K.S., Singh,M. and Gutierrez-Hartmann,A., et al.
     The ETS transcription factor ESE-1 transforms MCF-12A human mammary epithelial cells via a novel cytoplasmic mechanism
[6] Oettgen P., Alani R.M., Barcinski M.A., Brown L., Akbarali Y., Boltax J., Kunsch C., Munger K., Libermann T.A.
     Isolation and characterization of a novel epithelium-specific transcription factor, ESE-1, a member of the ets family.
[7] Andreoli J.M., Jang S.-I., Chung E., Coticchia C.M., Steinert P.M., Markova N.G.
     The expression of a novel, epithelium-specific ets transcription factor is restricted to the most differentiated layers in the epidermis.
[8] Chang C.-H., Scott G.K., Kuo W.-L., Xiong X., Suzdaltseva Y., Park J.W., Sayre P., Erny K., Collins C., Gray J.W., et al.
     ESX: a structurally unique Ets overexpressed early during human breast tumorigenesis.
[9] Tymms M.J., Ng A.Y.N., Thomas R.S., Schutte B.C., Zhou J., Eyre H.J., Sutherland G.R., Seth A., Rosenberg M., Papas T., et al.
     A novel epithelial-expressed ETS gene, ELF3: human and murine cDNA sequences, murine genomic organization, human mapping to 1q32.2 and expression in tissues and cancer.
[10] Choi S.-G., Yi Y., Kim Y.-S., Kato M., Chang J., Chung H.-W., Hahm K.-B., Yang H.-K., Rhee H.H., Bang Y.-J., et al.
     A novel ets-related transcription factor, ERT/ESX/ESE-1, regulates expression of the transforming growth factor-beta type II receptor.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: ELF3 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of ELF3 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about ELF3 antibody, all related information, articles, resources about ELF3 antibody are being stored on our online database. Let us know if you have questions regarding this product.

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