AOF2 antibody, amine oxidase (flavin containing) domain 2 (AOF2),transcript variant 2 Antibody, Mouse Monoclonal Antibody

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amine oxidase (flavin containing) domain 2 (AOF2),transcript variant 2 Antibody, Mouse Monoclonal Antibody

Catalog Number: 20-372-60153

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Related Product Names:

- AOF2 antibody; AOF2; LSD1; BHC110; KIAA0601; RP1-184J9.1; amine oxidase (flavin containing) domain 2 (AOF2),transcript variant 2
- Mouse monoclonal anti-human AOF2 antibody; EC 1.-.-.-; Flavin-containing amine oxidase domain-containing protein 2; BRAF35-HDAC complex protein BHC110; Homo sapiens amine oxidase (flavin containing) domain 2 (AOF2), transcript variant 2, mRNA; Lysine-specific histone demethylase 1

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: AOF2	Gene Name Synonym: LSD1; BHC110; KIAA0601; RP1-184J9.1
Gi #: 58761545	NCBI Acc #: NM_015013.2	Swiss Prot Acc #: O60341
Length (aa): N/A	Mol. Weight (Da): 92903	Chrom Location: N/A

Clone: 2026C5a

IGG Subclass: IgG1

Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.

Sterility: Filtered through a 0.22 um membrane.

Source/Host: Mouse

Clonality: Monoclonal

Crossreactivity: Human

Format: Mouse monoclonal anti-human AOF2 antibody in PBS (3.0 mM KCl, 1.5 mM K2HPO4, 140 mM NaCl, 8.0 mM Na2HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3)

Storage: Store at 2-8 C for up to one year. We recommend storing at –20 C for long-term storage. Avoid repeat freezing and thawing cycles.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for AOF2 ANTIBODY:

DB: Tested

TESTING: (secondary reagents and protocols )

Western Blot ( anti-AOF2 )

AOF2 antibody tern B image
AOF2

AOF2 ANTIBODY TARGET DESCRIPTION:

Synonym Names for AOF2 antibody: AOF2; LSD1; BHC110; KIAA0601; RP1-184J9.1; Mouse monoclonal anti-human AOF2 antibody; EC 1.-.-.-; Flavin-containing amine oxidase domain-containing protein 2; BRAF35-HDAC complex protein BHC110; Homo sapiens amine oxidase (flavin containing) domain 2 (AOF2), transcript variant 2, mRNA; Lysine-specific histone demethylase 1

Function: Histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and tri-methylted 'Lys-4' of histone H3. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. May also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.

Cofactor: FAD.

Biophysicochemical Properties:

Subunit: Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, AOF2/LSD1, RCOR1/CoREST and PHF21A/BHC80. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. In the complex, RCOR1/CoREST strongly enhances the demethylase activity and protects it from the proteasome while PHF21A/BHC80 inhibits the demethylase activity. Interacts with the androgen receptor (AR).

Subcellular Location: Nucleus.

Tissue Specificity: Ubiquitously expressed.

Domain: The SWIRN domain may act as an anchor site for a histone tail.

Similarity: Belongs to the flavin monoamine oxidase family.

Similarity: Contains 1 SWIRM domain.

Summary: This protein is a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternate splicing has been observed; however, not all transcript variants have been fully characterized.

amine oxidase (flavin containing) domain 2 (AOF2),transcript variant 2 reacts with human.

OMIM: 609132; gene. [NCBI / EBI]

PRODUCT INTERACTIONS:

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

BACKGROUND REFERENCES for AOF2 ANTIBODY:

Background references for antibody target are not specific to GenWay products

[1] Forneris,F., Binda,C., Adamo,A., Battaglioli,E. and Mattevi,A., et al.
     Structural basis of LSD1-CoREST selectivity in histone H3 recognition
[2] Stavropoulos,P. and Hoelz,A., et al.
     Lysine-specific demethylase 1 as a potential therapeutic target
[3] Scoumanne,A. and Chen,X., et al.
     The lysine-specific demethylase 1 is required for cell proliferation in both p53-dependent and -independent manners
[4] Schmidt,D.M. and McCafferty,D.G., et al.
     trans-2-Phenylcyclopropylamine is a mechanism-based inactivator of the histone demethylase LSD1
[5] Wissmann,M., Yin,N., Muller,J.M., Greschik,H., Fodor,B.D., Jenuwein,T., Vogler,C., Schneider,R., Gunther,T., Buettner,R.,, et al.
     Cooperative demethylation by JMJD2C and LSD1 promotes androgen receptor-dependent gene expression
[6] Nagase T., Ishikawa K., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
     Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro.
[7] Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., et al.
     The DNA sequence and biological annotation of human chromosome 1.
[8] Hakimi M.-A., Bochar D.A., Chenoweth J., Lane W.S., Mandel G., Shiekhattar R.
     A core-BRAF35 complex containing histone deacetylase mediates repression of neuronal-specific genes.
[9] Humphrey G.W., Wang Y., Russanova V.R., Hirai T., Qin J., Nakatani Y., Howard B.H.
     Stable histone deacetylase complexes distinguished by the presence of SANT domain proteins CoREST/kiaa0071 and Mta-L1.
[10] Hakimi M.-A., Dong Y., Lane W.S., Speicher D.W., Shiekhattar R.
     A candidate X-linked mental retardation gene is a component of a new family of histone deacetylase-containing complexes.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: AOF2 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of AOF2 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about AOF2 antibody, all related information, articles, resources about AOF2 antibody are being stored on our online database. Let us know if you have questions regarding this product.

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