CHEK1 antibody, CHK1 checkpoint homolog (S. pombe) (CHEK1) Antibody, Mouse Monoclonal Antibody

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CHK1 checkpoint homolog (S. pombe) (CHEK1) Antibody, Mouse Monoclonal Antibody

Catalog Number: 20-372-60186

Buy CHEK1 antibody -

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Related Product Names:

- CHEK1 antibody; CHEK1; CHK1; CHK1 checkpoint homolog (S. pombe) (CHEK1)
- Mouse monoclonal anti-human 2178C1a antibody; EC 2.7.11.1; Homo sapiens CHK1 checkpoint homolog (S. pombe) (CHEK1), mRNA; Serine/threonine-protein kinase Chk1

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: CHEK1	Gene Name Synonym: CHK1
Gi #: 141803244	NCBI Acc #: NM_001274.3	Swiss Prot Acc #: O14757
Length (aa): N/A	Mol. Weight (Da): 54420	Chrom Location: N/A

Clone: 2178C1a

IGG Subclass: IgG1

Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.

Sterility: Filtered through a 0.22 um membrane.

Source/Host: Mouse

Clonality: Monoclonal

Crossreactivity: Human

Format: Mouse monoclonal anti-human 2178C1a antibody in PBS (3.0 mM KCl, 1.5 mM K2HPO4, 140 mM NaCl, 8.0 mM Na2HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3).

Storage: Store at 2-8 C for up to one year. We recommend storing at –20 C for long-term storage. Avoid repeat freezing and thawing cycles.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for CHEK1 ANTIBODY:

DB: Tested

TESTING: (secondary reagents and protocols )

Western Blot ( anti-CHEK1 )

CHEK1 antibody tern B image
CHEK1

CHEK1 ANTIBODY TARGET DESCRIPTION:

Synonym Names for CHEK1 antibody: CHEK1; CHK1; Mouse monoclonal anti-human 2178C1a antibody; EC 2.7.11.1; Homo sapiens CHK1 checkpoint homolog (S. pombe) (CHEK1), mRNA; Serine/threonine-protein kinase Chk1

Function: Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation.

Catalytic Activity: ATP + a protein = ADP + a phosphoprotein.

Subunit: Interacts with BRCA1, CLSPN, PPM1D, RAD51, TIMELESS, XPO1/CRM1 and YWHAZ/14-3-3 zeta.

Subcellular Location: Nucleus. Cytoplasm. Note=Nuclear export is mediated at least in part by XPO1/CRM1. Also localizes to the centrosome specifically during interphase where it may protect centrosomal CDC2 kinase from inappropriate activation by cytoplasmic CDC25B.

Tissue Specificity: Expressed ubiquitously with the most abundant expression in thymus, testis, small intestine and colon.

Domain: The autoinhibitory region (AIR) inhibits the activity of the kinase domain.

Ptm: Phosphorylated by ATR in a RAD17-dependent manner in response to ultraviolet irradiation and inhibition of DNA replication. Phosphorylated by ATM in response to ionizing irradiation. ATM and ATR can both phosphorylate Ser-317 and Ser-345 and this results in enhanced kinase activity. Phosphorylation at Ser-345 also increases binding to 14-3-3 proteins and promotes nuclear retention. Conversely, dephosphorylation at Ser-345 by PPM1D may contribute to exit from checkpoint mediated cell cycle arrest. May also be phosphorylated at Ser-280 by AKT1/PKB, which may promote mono and/or diubiquitination. Also phosphorylated at undefined residues during mitotic arrest, which results in decreased activity.

Ptm: Ubiquitinated. Mono or diubiquitination promotes nuclear exclusion (By similarity).

Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily.

Similarity: Contains 1 protein kinase domain.

CHK1 checkpoint homolog (S. pombe) (CHEK1) reacts with human.

OMIM: 603078; gene. [NCBI / EBI]

Pathways:
KEGG pathway: Cell cycle 04110
Reactome Event:Cell Cycle Checkpoints 69620

Products similar to CHEK1 antibody:

IgG

CHEK1, IgG

Chk1 [6F5], IgG

CHEK1, IgG

Chk1 (Ab-317), IgG

Chk1, IgG

Peptide

Chk1 (Phospho-Ser345) Antibody Blocking Peptide, Peptide

Serine/threonine-protein kinase Chk1, Protein

PRODUCT INTERACTIONS:

CHK1 checkpoint homolog (S. pombe) (CHEK1) interacts with AATF (apoptosis antagonizing transcription factor), IgG (GenWay Catalog #: 20-372-60186).

CHK1 checkpoint homolog (S. pombe) (CHEK1) interacts with apoptosis antagonizing transcription factor, IgG (GenWay Catalog #: 20-372-60186).

CHK1 checkpoint homolog (S. pombe) (CHEK1) interacts with AATF (apoptosis antagonizing transcription factor), IgG (GenWay Catalog #: 20-372-60186).

CHK1 checkpoint homolog (S. pombe) (CHEK1) interacts with Cdc25A, IgG (GenWay Catalog #: 20-372-60186).

CHK1 checkpoint homolog (S. pombe) (CHEK1) interacts with CDC25A, IgG (GenWay Catalog #: 20-372-60186).

CHK1 checkpoint homolog (S. pombe) (CHEK1) interacts with CDC25A [DCS-120], IgG (GenWay Catalog #: 20-372-60186).

CHK1 checkpoint homolog (S. pombe) (CHEK1) interacts with cdc25A (Phospho-Ser75), IgG (GenWay Catalog #: 20-372-60186).

CHK1 checkpoint homolog (S. pombe) (CHEK1) interacts with cdc25A (Ab-75), IgG (GenWay Catalog #: 20-372-60186).

CHK1 checkpoint homolog (S. pombe) (CHEK1) interacts with cdc25A (Phospho-Ser75) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 20-372-60186).

CHK1 checkpoint homolog (S. pombe) (CHEK1) interacts with Cdc25A, IgG (GenWay Catalog #: 20-372-60186).

CHK1 checkpoint homolog (S. pombe) (CHEK1) interacts with cell division cycle 25 homolog C (S. cerevisiae) (CDC25C), IgG (GenWay Catalog #: 20-372-60186).

CHK1 checkpoint homolog (S. pombe) (CHEK1) interacts with cdc25C (Phospho-Ser216), IgG (GenWay Catalog #: 20-372-60186).

CHK1 checkpoint homolog (S. pombe) (CHEK1) interacts with cdc25C (Ab-216), IgG (GenWay Catalog #: 20-372-60186).

CHK1 checkpoint homolog (S. pombe) (CHEK1) interacts with cdc25C (Phospho-Ser216) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 20-372-60186).

CHK1 checkpoint homolog (S. pombe) (CHEK1) interacts with CDC25C [DCS-193], IgG (GenWay Catalog #: 20-372-60186).

CHK1 checkpoint homolog (S. pombe) (CHEK1) interacts with HSPCB, IgG (GenWay Catalog #: 20-372-60186).

CHK1 checkpoint homolog (S. pombe) (CHEK1) interacts with HSP90B (Ab-254), IgG (GenWay Catalog #: 20-372-60186).

CHK1 checkpoint homolog (S. pombe) (CHEK1) interacts with HSP90AB1 (heat shock protein 90kDa alpha (cytosolic). class B member 1), IgG (GenWay Catalog #: 20-372-60186).

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

BACKGROUND REFERENCES for CHEK1 ANTIBODY:

Background references for antibody target are not specific to GenWay products

[1] Kim,B.M., Choi,J.Y., Kim,Y.J., Woo,H.D. and Chung,H.W., et al.
     Reoxygenation following hypoxia activates DNA-damage checkpoint signaling pathways that suppress cell-cycle progression in cultured human lymphocytes
[2] Kim,C.J., Lee,J.H., Song,J.W., Cho,Y.G., Kim,S.Y., Nam,S.W., Yoo,N.J., Park,W.S. and Lee,J.Y.
     Chk1 frameshift mutation in sporadic and hereditary non-polyposis colorectal cancers with microsatellite instability
[3] Kim,M.A., Kim,H.J., Brown,A.L., Lee,M.Y., Bae,Y.S., Park,J.I., Kwak,J.Y., Chung,J.H. and Yun,J.
     Identification of novel substrates for human checkpoint kinase Chk1 and Chk2 through genome-wide screening using a consensus Chk phosphorylation motif
[4] Wang,X., Kennedy,R.D., Ray,K., Stuckert,P., Ellenberger,T. and D'Andrea,A.D.
     Chk1-mediated phosphorylation of FANCE is required for the Fanconi anemia/BRCA pathway
[5] Reinhardt,H.C., Aslanian,A.S., Lees,J.A. and Yaffe,M.B., et al.
     p53-deficient cells rely on ATM- and ATR-mediated checkpoint signaling through the p38MAPK/MK2 pathway for survival after DNA damage
[6] Sanchez Y., Wong C., Thoma R.S., Richman R., Wu Z., Piwnica-Worms H., Elledge S.J.
     Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25.
[7] Flaggs G., Plug A.W., Dunks K.M., Mundt K.E., Ford J.C., Quiggle M.R.E., Taylor E.M., Westphal C.H., Ashley T., Hoekstra M.F., et al.
     Atm-dependent interactions of a mammalian chk1 homolog with meiotic chromosomes.
[8] Semba S., Ouyang H., Han S.-Y., Kato Y., Horii A.
     Analysis of the candidate target genes for mutation in microsatellite instability-positive cancers of the colorectum, stomach, and endometrium.
[9] Rieder M.J., Livingston R.J., Braun A.C., Montoya M.A., Chung M.-W., Miyamoto K.E., Nguyen C.P., Nguyen D.A., Poel C.L., Robertson P.D., et al.
     NIEHS-SNPs, environmental genome project, NIEHS ES15478, Department of Genome Sciences, Seattle, WA (URL: http://egp.gs.washington.edu).
[10] Shieh S.-Y., Ahn J., Tamai K., Taya Y., Prives C.
     The human homologs of checkpoint kinases Chk1 and Cds1 (Chk2) phosphorylate p53 at multiple DNA damage-inducible sites.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: CHEK1 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of CHEK1 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about CHEK1 antibody, all related information, articles, resources about CHEK1 antibody are being stored on our online database. Let us know if you have questions regarding this product.

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