RUVBL1 antibody, RuvB-like 1 (E. coli) (RUVBL1) Antibody, Mouse Monoclonal Antibody

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RuvB-like 1 (E. coli) (RUVBL1) Antibody, Mouse Monoclonal Antibody

Catalog Number: 20-372-60278

Buy RUVBL1 antibody -

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Related Product Names:

- RUVBL1 antibody; RUVBL1; RVB1; ECP54;TIP49; NMP238; TIP49A; RuvB-like 1 (E. coli) (RUVBL1)
- Mouse monoclonal anti-human RUVBL1 antibody; EC 3.6.1.-; 49 kDa TATA box-binding protein-interacting protein; 49 kDa TBP-interacting protein; TIP49a; Pontin 52; Nuclear matrix protein 238; NMP 238; 54 kDa erythrocyte cytosolic protein; ECP-54; TIP60-assoc; Homo sapiens RuvB-like 1 (E. coli) (RUVBL1), mRNA; RuvB-like 1

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: RUVBL1	Gene Name Synonym: RVB1; ECP54;TIP49; NMP238; TIP49A
Gi #: 4506752	NCBI Acc #: NM_003707.1	Swiss Prot Acc #: Q9Y265
Length (aa): N/A	Mol. Weight (Da): 50228	Chrom Location: N/A

Clone: 2943C1a

IGG Subclass: IgG1

Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.

Sterility: Filtered through a 0.22 um membrane.

Source/Host: Mouse

Clonality: Monoclonal

Crossreactivity: Human

Format: Mouse monoclonal anti-human RUVBL1 antibody in PBS (3.0 mM KCl, 1.5 mM K2HPO4, 140 mM NaCl, 8.0 mM Na2HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3)

Storage: Store at 2-8 C for up to one year. We recommend storing at –20 C for long-term storage. Avoid repeat freezing and thawing cycles.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for RUVBL1 ANTIBODY:

DB, Flow Cytometry (FACS), WB: Tested

TESTING: (secondary reagents and protocols )

Flow Cytometry ( anti-RUVBL1 )

RUVBL1 antibody w Cytome image
RUVBL1

Western Blot ( anti-RUVBL1 )

RUVBL1 antibody tern B image
RUVBL1

Western Blot ( anti-RUVBL1 )

RUVBL1 antibody tern B image
RUVBL1

RUVBL1 ANTIBODY TARGET DESCRIPTION:

Synonym Names for RUVBL1 antibody: RUVBL1; RVB1; ECP54;TIP49; NMP238; TIP49A; Mouse monoclonal anti-human RUVBL1 antibody; EC 3.6.1.-; 49 kDa TATA box-binding protein-interacting protein; 49 kDa TBP-interacting protein; TIP49a; Pontin 52; Nuclear matrix protein 238; NMP 238; 54 kDa erythrocyte cytosolic protein; ECP-54; TIP60-assoc; Homo sapiens RuvB-like 1 (E. coli) (RUVBL1), mRNA; RuvB-like 1

Function: Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex.

Function: May be able to bind plasminogen at cell surface and enhance plasminogen activation.

Function: Essential for cell proliferation.

Subunit: Forms homotypic and heterotypic interactions. Forms a multimeric complex with RUVBL2. Interacts with the transcriptional activation domain of MYC. Component of the RNA polymerase II holoenzyme complex. May also act to bridge the LEF1/TCF1-CTNNB1 complex and TBP. Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit HTATIP/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41 and VPS72/YL1. The NuA4 complex interacts with MYC and the adenovirus E1A protein. RUVBL1 interacts with EP400. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, TCFL1/YL1 and YEATS4/GAS41. Component of the BAF53 complex, at least composed of ACTL6A/BAF53A, RUVBL1/TIP49, SMARCA2/BRM, and TRRAP/PAF400. Associates with alpha and gamma tubulins, particularly during metaphase and early anaphase.

Subcellular Location: Nucleus matrix. Nucleus, nucleoplasm. Cytoplasm. Membrane. Note=Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol, although it is also present in the cytoplasm and associated with the cell membranes. In prophase and prometaphase it is located at the centrosome and the branching microtubule spindles. After mitotic nuclear membrane disintigration it accumulates at the centrosome and sites of tubulin polymerization. As cells pass through metaphase and into telophase it is located close to the centrosome at the early phase of tubulin polymerization. In anaphase it accumulates at the zone of tubule interdigitation. In telophase it is found at polar tubule overlap, and it reappears at the site of chromosomal decondensation in the daughter cells.

Tissue Specificity: Ubiquitously expressed with high expression in heart, skeletal muscle and testis.

Domain: Binding to MYC is dependent on a Myc domain essential for oncogenic activity.

Miscellaneous: High level of autoantibodies against RUVBL1 are detected in sera of patients with autoimmune diseases such as polymyositis/dermatomyosistis and autoimmune hepatitis.

Similarity: Belongs to the ruvB family.

RuvB-like 1 (E. coli) (RUVBL1) reacts with human.

OMIM: 603449; gene. [NCBI / EBI]

PRODUCT INTERACTIONS:

RuvB-like 1 (E. coli) (RUVBL1) interacts with RUVBL2 (RuvB-like 2 (E. coli)), IgG (GenWay Catalog #: 20-372-60278).

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

BACKGROUND REFERENCES for RUVBL1 ANTIBODY:

Background references for antibody target are not specific to GenWay products

[1] Puri,T., Wendler,P., Sigala,B., Saibil,H. and Tsaneva,I.R., et al.
     Dodecameric structure and ATPase activity of the human TIP48/TIP49 complex
[2] Matias,P.M., Gorynia,S., Donner,P. and Carrondo,M.A., et al.
     Crystal structure of the human AAA+ protein RuvBL1
[3] Jin,J., Cai,Y., Yao,T., Gottschalk,A.J., Florens,L., Swanson,S.K., Gutierrez,J.L., Coleman,M.K., Workman,J.L., Mushegian,A.,, et al.
     A mammalian chromatin remodeling complex with similarities to the yeast INO80 complex
[4] Cai,Y., Jin,J., Florens,L., Swanson,S.K., Kusch,T., Li,B., Workman,J.L., Washburn,M.P., Conaway,R.C. and Conaway,J.W.
     The mammalian YL1 protein is a shared subunit of the TRRAP/TIP60 histone acetyltransferase and SRCAP complexes
[5] Andersen,J.S., Lam,Y.W., Leung,A.K., Ong,S.E., Lyon,C.E., Lamond,A.I. and Mann,M.
     Nucleolar proteome dynamics
[6] Makino Y., Mimori T., Koike C., Kanemaki M., Kurokawa Y., Inoue S., Kishimoto T., Tamura T.-A.
     TIP49, homologous to the bacterial DNA helicase RuvB, acts as an autoantigen in human.
[7] Holzmann K., Gerner C., Korosec T., Poeltl A., Grimm R., Sauermann G.
     Identification and characterization of the ubiquitously occurring nuclear matrix protein NMP 238.
[8] Qiu X.-B., Lin Y.-L., Thome K.C., Pian P., Schlegel B.P., Weremowicz S., Parvin J.D., Dutta A.
     An eukaryotic RuvB-like protein (RUVBL1) essential for growth.
[9] Bauer A., Huber O., Kemler R.
     Pontin52, an interaction partner of beta-catenin, binds to the TATA box binding protein.
[10] Salzer U., Kubicek M., Prohaska R.
     Isolation, molecular characterization, and tissue-specific expression of ECP-51 and ECP-54 (TIP49), two homologous, interacting erythroid cytosolic proteins.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: RUVBL1 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of RUVBL1 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about RUVBL1 antibody, all related information, articles, resources about RUVBL1 antibody are being stored on our online database. Let us know if you have questions regarding this product.

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