CRK antibody, v-crk sarcoma virus CT10 oncogene homolog (avian) (CRK), transcript variant II Antibody, Mouse Monoclonal Antibody

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v-crk sarcoma virus CT10 oncogene homolog (avian) (CRK), transcript variant II Antibody, Mouse Monoclonal Antibody

Catalog Number: 20-372-60296

Buy CRK antibody -

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Related Product Names:

- CRK antibody; CRK; CRKII; v-crk sarcoma virus CT10 oncogene homolog (avian) (CRK), transcript variant II
- Mouse monoclonal anti-human CRK antibody; p38; Adapter molecule crk; Homo sapiens v-crk sarcoma virus CT10 oncogene homolog (avian) (CRK), transcript variant II, mRNA; Proto-oncogene C-crk

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: CRK	Gene Name Synonym: CRKII
Gi #: 41327711	NCBI Acc #: NM_016823.2	Swiss Prot Acc #: P46108
Length (aa): N/A	Mol. Weight (Da): 33831	Chrom Location: N/A

Clone: 2210C1a

IGG Subclass: IgG1

Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.

Sterility: Filtered through a 0.22 um membrane.

Source/Host: Mouse

Clonality: Monoclonal

Crossreactivity: Human

Format: Mouse monoclonal anti-human CRK antibody in PBS (3.0 mM KCl, 1.5 mM K2HPO4, 140 mM NaCl, 8.0 mM Na2HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3)

Storage: Store at 2-8 C for up to one year. We recommend storing at –20 C for long-term storage. Avoid repeat freezing and thawing cycles.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for CRK ANTIBODY:

DB: Tested

TESTING: (secondary reagents and protocols )

Western Blot ( anti-CRK )

CRK antibody tern B image
CRK

CRK ANTIBODY TARGET DESCRIPTION:

Synonym Names for CRK antibody: CRK; CRKII; Mouse monoclonal anti-human CRK antibody; p38; Adapter molecule crk; Homo sapiens v-crk sarcoma virus CT10 oncogene homolog (avian) (CRK), transcript variant II, mRNA; Proto-oncogene C-crk

Function: The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4.

Subunit: Interacts with ABL1, C3G, SOS, MAP4K1, MAPK8 and DOCK3 via its first SH3 domain. Interacts with BCAR1, CBL, CBLB, PXN and GAB1 via its SH2 domain upon stimulus-induced tyrosine phosphorylation. Interacts with several tyrosine-phosphorylated growth factor receptors such as EGFR, PDGFR and INSR via its SH2 domain (By similarity). Interacts with DOCK1 and DOCK4. Interacts with SHB.

Subcellular Location: Cytoplasm (By similarity). Cell membrane (By similarity). Note=Translocated to the plasma membrane upon cell adhesion (By similarity).

Domain: The C-terminal SH3 domain function as a negative modulator for transformation and the N-terminal SH3 domain appears to function as a positive regulator for transformation (By similarity).

Domain: The SH2 domain mediates interaction with SHB.

Ptm: Phosphorylation of Crk-II (40 kDa) gives rise to a 42 kDa form.

Ptm: Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway.

Similarity: Contains 1 SH2 domain.

Similarity: Contains 2 SH3 domains.

Summary: This protein is a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.

v-crk sarcoma virus CT10 oncogene homolog (avian) (CRK), transcript variant II reacts with human.

OMIM: 164762; gene. [NCBI / EBI]

Pathways:
KEGG pathway: Chronic myeloid leukemia 05220
KEGG pathway: ErbB signaling pathway 04012
KEGG pathway: Focal adhesion 04510
KEGG pathway: Insulin signaling pathway 04910
KEGG pathway: MAPK signaling pathway 04010
KEGG pathway: Regulation of actin cytoskeleton 04810
KEGG pathway: Renal cell carcinoma 05211

Products similar to CRK antibody:

IgG

CRKL (v-crk sarcoma virus CT10 oncogene homolog (avian)-like), IgG

CDK6 [DCS-90], IgG

GNB1L (guanine nucleotide binding protein (G protein). beta polypeptide 1-like), IgG

BCAR1, IgG

CDK4 [DCS-31], IgG

PRODUCT INTERACTIONS:

v-crk sarcoma virus CT10 oncogene homolog (avian) (CRK), transcript variant II interacts with KH domain-containing, RNA-binding, signal transduction-associated protein 1, IgG (GenWay Catalog #: 20-372-60296).

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

BACKGROUND REFERENCES for CRK ANTIBODY:

Background references for antibody target are not specific to GenWay products

[1] Brandt,S., Shafikhani,S., Balachandran,P., Jin,S., Hartig,R., Konig,W., Engel,J. and Backert,S.
     Use of a novel coinfection system reveals a role for Rac1, H-Ras, and CrkII phosphorylation in Helicobacter pylori-induced host cell actin cytoskeletal rearrangements
[2] Lee,W.L., Cosio,G., Ireton,K. and Grinstein,S., et al.
     Role of CrkII in Fcgamma receptor-mediated phagocytosis
[3] Olsen,J.V., Blagoev,B., Gnad,F., Macek,B., Kumar,C., Mortensen,P. and Mann,M.
     Global, in vivo, and site-specific phosphorylation dynamics in signaling networks
[4] Watanabe,T., Tsuda,M., Makino,Y., Ichihara,S., Sawa,H., Minami,A., Mochizuki,N., Nagashima,K. and Tanaka,S.
     Adaptor molecule Crk is required for sustained phosphorylation of Grb2-associated binder 1 and hepatocyte growth factor-induced cell motility of human synovial sarcoma cell lines
[5] Lim,J., Hao,T., Shaw,C., Patel,A.J., Szabo,G., Rual,J.F., Fisk,C.J., Li,N., Smolyar,A., Hill,D.E., Barabasi,A.L., Vidal,M., et al.
     A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration
[6] Matsuda M., Tanaka S., Nagata S., Kojima A., Kurata T., Shibuya M.
     Two species of human CRK cDNA encode proteins with distinct biological activities.
[7] Fioretos T., Heisterkamp N., Groffen J., Benjes S., Morris C.
     CRK proto-oncogene maps to human chromosome band 17p13.
[8] Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., et al.
     Cloning of human full-length CDSs in BD Creator(TM) system donor vector.
[9] Hasegawa H., Kiyokawa E., Tanaka S., Nagashima K., Gotoh N., Shibuya M., Kurata T., Matsuda M.
     DOCK180, a major CRK-binding protein, alters cell morphology upon translocation to the cell membrane.
[10] Matsuda M., Ota S., Tanimura R., Nakamura H., Matuoka K., Takenawa T., Nagashima K., Kurata T.
     Interaction between the amino-terminal SH3 domain of CRK and its natural target proteins.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: CRK antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of CRK antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about CRK antibody, all related information, articles, resources about CRK antibody are being stored on our online database. Let us know if you have questions regarding this product.

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