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| ZW10, kinetochore associated, homolog (Drosophila) (ZW10) Antibody, Mouse Monoclonal Antibody |
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| Catalog Number: 20-372-60315 |
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| Related Product Names:
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ZW10 antibody; ZW10; HZW10; KNTC1AP; MGC149821; ZW10, kinetochore associated, homolog (Drosophila) (ZW10)
- Mouse monoclonal anti-human ZW10 antibody; N/A; Homo sapiens ZW10, kinetochore associated, homolog (Drosophila) (ZW10), mRNA; Centromere/kinetochore protein zw10 homolog |
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- Gene Information -
Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.
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| Gene Name: ZW10 |
Gene Name Synonym: HZW10; KNTC1AP; MGC149821 |
Gi #:
17136150 |
NCBI Acc #:
NM_004724.2 |
Swiss Prot Acc #:
O43264 |
Length (aa): N/A |
Mol. Weight (Da): 88829 |
Chrom Location: N/A |
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 Clone: 3363C4a
IGG Subclass: IgG1
Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility: Filtered through a 0.22 um membrane.
Source/Host: Mouse
Clonality: Monoclonal
Crossreactivity: Human
Format: Mouse monoclonal anti-human ZW10 antibody in PBS (3.0 mM KCl, 1.5 mM K2HPO4, 140 mM NaCl, 8.0 mM Na2HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3).
Storage: Store at 2-8 C for up to one year. We recommend storing at –20 C for long-term storage. Avoid repeat freezing and thawing cycles.
Shipping: Products may be shipped on ice pack or dry ice. |
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| APPLICATIONS for ZW10 ANTIBODY: |
| DB: Tested |
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| ZW10 ANTIBODY TARGET DESCRIPTION: |
Synonym Names for ZW10 antibody: ZW10; HZW10; KNTC1AP; MGC149821; Mouse monoclonal anti-human ZW10 antibody; N/A; Homo sapiens ZW10, kinetochore associated, homolog (Drosophila) (ZW10), mRNA; Centromere/kinetochore protein zw10 homolog
Function: Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin complex onto kinetochores. Involved in regulation of membrane traffic between the Golgi and the endoplasmic reticulum.
Subunit: Associated with a SNARE complex consisting of STX18, USE1L, BNIP1/SEC20L, and SEC22B through direct interaction with RINT1/TIP20L bound to BNIP1/SEC20L. Interacts with C19orf25, KNTC1, ZWILCH and ZWINT.
Subcellular Location: Cytoplasm. Endoplasmic reticulum membrane; Peripheral membrane protein. Kinetochore. Spindle. Note=Dynamic pattern of localization during the cell cycle. In most cells at interphase, present diffusely in the cytoplasm. In prometaphase, associated with the kinetochore. At metaphase, detected both at the kinetochores and, most prominently, at the spindle, particularly at the spindle poles. In very early anaphase, detected on segregating kinetochores. In late anaphase and telophase, accumulates at the spindle midzone.
Tissue Specificity: Widely expressed.
Developmental Stage: No significant variation in expression during cell cycle.
Miscellaneous: Overexpression as well as silencing of ZW10 disrupts the morphology of the ER-Golgi intermediate compartment as well as the Golgi apparatus and slows down ER-Golgi transport.
Similarity: Belongs to the ZW10 family.
Summary: This protein is a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. The encoded protein binds to centromeres during the prophase, metaphase, and early anaphase cell division stages and to kinetochore microtubules during metaphase.
ZW10, kinetochore associated, homolog (Drosophila) (ZW10) reacts with human.
OMIM: 603954; gene. [NCBI / EBI]
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| PRODUCT INTERACTIONS: |
ZW10, kinetochore associated, homolog (Drosophila) (ZW10) interacts with Gstk1, IgG (GenWay Catalog #: 20-372-60315).
ZW10, kinetochore associated, homolog (Drosophila) (ZW10) interacts with Gstk1, IgG (GenWay Catalog #: 20-372-60315).
ZW10, kinetochore associated, homolog (Drosophila) (ZW10) interacts with Recombinant Human Glutathione S-Transferase Kappa 1, Protein (GenWay Catalog #: 20-372-60315).
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SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.
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| BACKGROUND
REFERENCES for ZW10 ANTIBODY: |
| Background references for antibody target are not specific to GenWay products |
[1] Arasaki,K., Uemura,T., Tani,K. and Tagaya,M., et al.
Correlation of Golgi localization of ZW10 and centrosomal accumulation of dynactin
[2] Lin,Y.T., Chen,Y., Wu,G. and Lee,W.H., et al.
Hec1 sequentially recruits Zwint-1 and ZW10 to kinetochores for faithful chromosome segregation and spindle checkpoint control
[3] Arasaki,K., Taniguchi,M., Tani,K. and Tagaya,M., et al.
RINT-1 regulates the localization and entry of ZW10 to the syntaxin 18 complex
[4] Nousiainen,M., Sillje,H.H., Sauer,G., Nigg,E.A. and Korner,R., et al.
Phosphoproteome analysis of the human mitotic spindle
[5] Varma,D., Dujardin,D.L., Stehman,S.A. and Vallee,R.B., et al.
Role of the kinetochore/cell cycle checkpoint protein ZW10 in interphase cytoplasmic dynein function
[6] Starr D.A., Williams B.C., Li Z., Etemad-Moghadam B., Dawe R.K., Goldberg M.L.
Conservation of the centromere/kinetochore protein ZW10.
[7] Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.
Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.
[8] Scaeerou F., Starr D.A., Piano F., Papoulas O., Karess R.E., Goldberg M.L.
The ZW10 and Rough Deal checkpoint proteins function together in a large, evolutionarily conserved complex targeted to the kinetochore.
[9] Hirose H., Arasaki K., Dohmae N., Takio K., Hatsuzawa K., Nagahama M., Tani K., Yamamoto A., Tohyama M., Tagaya M., et al.
Implication of ZW10 in membrane trafficking between the endoplasmic reticulum and Golgi.
[10] Nakajima K., Hirose H., Taniguchi M., Kurashina H., Arasaki K., Nagahama M., Tani K., Yamamoto A., Tagaya M.
Involvement of BNIP1 in apoptosis and endoplasmic reticulum membrane fusion. |
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Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.
Precautions: ZW10 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.
Important Notes: During shipment, small volumes of ZW10 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.
Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about ZW10 antibody, all related information, articles, resources about ZW10 antibody are being stored on our online database. Let us know if you have questions regarding this product.
Disclaimer: For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed. |
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