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| MHC CLASS II MONOMORPHIC Antibody, Rat Monoclonal Antibody |
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| Catalog Number: 20-783-70066 |
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| Related Product Names:
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HLA-DRB1 antibody; HLA-DRB1; MHC CLASS II MONOMORPHIC
- RAT ANTI DOG MHC CLASS II MONOMORPHIC; Major histocompatibility complex, class II, DR beta 1; MHC class II antigen |
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- Gene Information -
Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.
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| Gene Name: HLA-DRB1 |
Gene Name Synonym: N/A |
Gi #:
4504411 |
NCBI Acc #:
NP_002115.1 |
Swiss Prot Acc #:
N/A |
Length (aa): N/A |
Mol. Weight (Da): 10864 |
Chrom Location: N/A |
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 Specificity: MHC CLASS II MONOMORPHIC
Isotype: Specificity: MHC CLASS II MONOMORPHIC
Clone: YKIX334.2
Immunogen: Con A activated canine peripheral blood cells.
Fusion Partner: Spleen cells from immunised DA rats were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.
Specificity Note: This product recognises canine MHC Class II and was classified at the First Canine Leucocyte Antigen Workshop. The antibody immunoprecipitates an antigen of 32/34kD and blocks the proliferation of MHC Class II dependent responses in vitro. In dogs, MHC Class II is expressed by all peripheral blood mononuclear cells.
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers:
Suggested Dilution: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Suggested Dilution: Flow Cytometry - 1/50 - 1/100
Source/Host: Rat
Clonality: Monoclonal
Crossreactivity: Dog
Format: Purified
Storage: Store at +4Cor at -20Cif preferred.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Stability: 18 months from date of despatch.
Shipping: Products may be shipped on ice pack or dry ice. |
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| APPLICATIONS for HLA-DRB1 ANTIBODY: |
| IHC-F, FACS/FC, IP: Tested |
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| TESTING: (secondary reagents and protocols
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| Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. ( anti-HLA-DRB1 )
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| HLA-DRB1 ANTIBODY TARGET DESCRIPTION: |
Synonym Names for HLA-DRB1 antibody: HLA-DRB1; RAT ANTI DOG MHC CLASS II MONOMORPHIC; Major histocompatibility complex, class II, DR beta 1; MHC class II antigen
Summary: HLA-DRB1 belongs to the HLA class II beta chain paralogues. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons, exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. DRB1 is present in all individuals. Allelic variants of DRB1 are linked with either none or one of the genes DRB3, DRB4 and DRB5. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9.
MHC CLASS II MONOMORPHIC reacts with dog.
OMIM: NCBI [142857]
Pathways:
KEGG pathway: Antigen processing and presentation 04612
KEGG pathway: Cell adhesion molecules (CAMs) 04514
KEGG pathway: Hematopoietic cell lineage 04640
KEGG pathway: Type I diabetes mellitus 04940
Products similar to HLA-DRB1 antibody:
IgG
 DRB1 (developmentally regulated RNA-binding protein 1), IgG
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SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.
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| BACKGROUND
REFERENCES for HLA-DRB1 ANTIBODY: |
| Background references for antibody target are not specific to GenWay products |
| 1. Cobbold. S. and Metcalfe. S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the first International Canine Leucocyte Antigen Workshop. 2. Watson. C.J.E. et al. (1994) Immunosuppression of canine renal allograft recipients by CD4 and CD8 monoclonal antibodies. |
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Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.
Precautions: HLA-DRB1 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.
Important Notes: During shipment, small volumes of HLA-DRB1 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.
Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about HLA-DRB1 antibody, all related information, articles, resources about HLA-DRB1 antibody are being stored on our online database. Let us know if you have questions regarding this product.
Disclaimer: For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed. |
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