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| 5-METHYLCYTIDINE Antibody, Mouse Monoclonal Antibody |
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| Catalog Number: 20-783-71663 |
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| Related Product Names:
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5-METHYLCYTIDINE antibody; 5-METHYLCYTIDINE
- MOUSE ANTI 5-METHYLCYTIDINE |
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- Gene Information -
Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.
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| Gene Name: N/A |
Gene Name Synonym: N/A |
Gi #:
N/A |
NCBI Acc #:
N/A |
Swiss Prot Acc #:
N/A |
Length (aa): N/A |
Mol. Weight (Da): N/A |
Chrom Location: N/A |
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 Specificity: 5-METHYLCYTIDINE
Isotype: IgG1
Clone: 33D3
Target Species: Broad
Flow Control: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Membrane permeabilisation may be required for this application. Cell pretreatment before staining is described in reference 4. Giraldo, A. M et al.
Fusion Partner: Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0-Ag 14 myeloma cell line.
Specificity Note: This product recognises the modified base 5-methylcytidine (5-MeCyd) found in DNA of plants and vertebrates. DNA methylation is a DNA modification process, which is involved in the control of gene expression. Reports suggest that in tumours, DNA is frequently globally hypomethylated compared to the DNA from normal tissue.
Clone 33D3 has been developed to discriminate between the modified base 5-MeCyd and the normal counterpart cytosine.
This product has been reported for use in Methylated DNA immunoprecipitation (MeDIP).
Immunohistology: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose.
Buffer Solution: Phosphate buffered saline pH7.4
Preservative Stabilisers: 0.01% Thimerosal
Suggested Dilution: Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Membrane permeabilisation may be required for this application. Cell pretreatment before staining is described in reference 4. Giraldo. A. M. et al.
Source/Host: Mouse
Clonality: Monoclonal
Crossreactivity: Reacts with: Human. Rat. Mouse N.B.Antibody reactivity and working conditions may vary between species.
Format: Purified
Storage: Store at +4Cor at -20Cif preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Stability: 18 months from date of despatch.
Shipping: Products may be shipped on ice pack or dry ice. |
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| APPLICATIONS for 5-METHYLCYTIDINE ANTIBODY: |
| IHC-F, FACS/FC, IB, IF, Methylated DNA IP: Tested |
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| TESTING: (secondary reagents and protocols
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| Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul Method sheets are available on request ( anti-5-METHYLCYTIDINE )
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| 5-METHYLCYTIDINE ANTIBODY TARGET DESCRIPTION: |
Synonym Names for 5-METHYLCYTIDINE antibody: N/A; MOUSE ANTI 5-METHYLCYTIDINE
5-METHYLCYTIDINE reacts with reacts with: human. rat. mouse n.b.antibody reactivity and working conditions may vary between species..
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SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.
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| BACKGROUND
REFERENCES for 5-METHYLCYTIDINE ANTIBODY: |
| Background references for antibody target are not specific to GenWay products |
| 1. Reynaud. C. et al. (1992) Monitoring of urinary excretion of modified nucleosides in cancer patients using a set of six monoclonal antibodies. 2. Habib. M. et al. (1999) DNA Global Hypomethylation in EBV - Transformed Interphase Nuclei. 3. Hernandez-Blazquez. F. et al. (2000) Evaluation of global DNA hypomethylation in human colon cancer tissues by immunohistochemistry and image analysis. |
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Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.
Precautions: 5-METHYLCYTIDINE antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.
Important Notes: During shipment, small volumes of 5-METHYLCYTIDINE antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.
Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about 5-METHYLCYTIDINE antibody, all related information, articles, resources about 5-METHYLCYTIDINE antibody are being stored on our online database. Let us know if you have questions regarding this product.
Disclaimer: For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed. |
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GenWay is a Protein and Antibody Solutions Provider. Customer Satisfaction is Our Top Priority.
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Thank you for visiting GenWay Biotech's Datasheet. GenWay is the world's leader in chicken, IgY antibody production, producing over 900 quality polyclonal IgY antibodies to date. From our unique recombinant protein production, utilizing a variety of protein expression systems, GenWay's polyclonal IgY antibodies incorporate more epitopes, increasing the success rate for ELISA, western blot, immunohistochemistry (IHC), immunocytochemistry (ICC), and many more immunoassays. |
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