|
|
| CD45 Antibody, Mouse Monoclonal Antibody |
 |
|
|
 |
| Catalog Number: 20-783-72637 |
|
|
|
| Related Product Names:
|
-
PTPRC antibody; PTPRC; CD45; CD45
- MOUSE ANTI HUMAN CD45: APC; CD45 [Takifugu rubripes]; Leukocyte common antigen |
|
- Gene Information -
Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.
|
|
| Gene Name: PTPRC |
Gene Name Synonym: CD45 |
Gi #:
74095921 |
NCBI Acc #:
NP_001027788.1 |
Swiss Prot Acc #:
P08575 |
Length (aa): N/A |
Mol. Weight (Da): 147254 |
Chrom Location: N/A |
|
|
 Specificity: CD45
NCBI Gene ID: 5788
Isotype: Specificity: CD45
Clone: F10-89-4
Immunogen: Human T lymphocytes
Fusion Partner: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Specificity Note: This product recognises the human CD45 cell surface antigen, also known as the leucocyte common antigen (LCA). CD45 is a complex molecule existing in a number of isoforms.
Antibodies recognising a common epitope on all of these isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
Clone F10-89-4 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes.
This product is routinely tested in flow cytometry on human peripheral blood leucocytes
Preparation: Purified IgG prepared by affinity chromatography from tissue culture supernatant
Buffer Solution: Phosphate buffered saline pH7.4
Preservative Stabilisers: | 0.09% | Sodium Azide | | 1% | Bovine Serum Albumin |
Reconstitution: Reconstitute with 1 ml distilled water
Suggested Dilution: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Suggested Dilution: Flow Cytometry - Neat
Source/Host: Mouse
Clonality: Monoclonal
Crossreactivity: Human
Format: APC
Storage: Prior to reconstitution store at +4 degree C. Following reconstitution store at +4 degree C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Stability: 12 months from date of reconstitution.
Shipping: Products may be shipped on ice pack or dry ice. |
|
| APPLICATIONS for PTPRC ANTIBODY: |
| FACS/FC: Tested |
|
|
| TESTING: (secondary reagents and protocols
) |
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood ( anti-PTPRC ) Flow Cytometry: Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD45:APC .
( anti-PTPRC )PTPRC antibody Flow Cytometry image
|
|
|
| PTPRC ANTIBODY TARGET DESCRIPTION: |
Synonym Names for PTPRC antibody: PTPRC; CD45; MOUSE ANTI HUMAN CD45: APC; CD45 [Takifugu rubripes]; Leukocyte common antigen
Function: Required for T-cell activation through the antigen receptor. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits an dephosphorylates SKAP1 and FYN.
Catalytic Activity: Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Subunit: Binds GANAB and PRKCSH (By similarity). Interacts with SKAP1.
Subcellular Location: Membrane; Single-pass type I membrane protein.
Domain: The first PTPase domain interacts with SKAP1.
Ptm: Heavily N- and O-glycosylated.
Disease: Defects in PTPRC are a cause of autosomal recessive severe combined immunodeficiency T-cell-negative/B-cell-positive/NK cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. SCID refers to a genetically and clinically group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms, including Candida albicans, Pneumocystis carinii, and cytomegalovirus, among many others. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Disease: Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
Similarity: Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Similarity: Contains 2 fibronectin type-III domains.
Similarity: Contains 2 tyrosine-protein phosphatase domains.
CD45 reacts with human.
OMIM: 126200; phenotype. [NCBI / EBI]
151460; gene. [NCBI / EBI]
608971; phenotype. [NCBI / EBI]
Products similar to PTPRC antibody:
IgG
 Mouse Anti-Mouse CD45.1/Ly-5.1, IgG
CD45RO, IgG
CD45R, IgG
CD45RO, IgG
CD45RB [B344 (BRA-11G)] (FITC), IgG
Protein
CD45 Human, Protein
CD45 (584-1256) HUMAN, Protein
Leukocyte common antigen, Protein
|
|
|
SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.
|
|
| BACKGROUND
REFERENCES for PTPRC ANTIBODY: |
| Background references for antibody target are not specific to GenWay products |
| 1. Dalchau. R. et al. (1980) Monoclonal antibody to a human leukocyte-specific membrane glycoprotein probably homologous to the leukocyte-common (L-C) antigen of the rat 2. Quenby. S et al. (1999) Pre-implantation endometrial leukocytes in women with recurrent miscarriage. |
|
Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.
Precautions: PTPRC antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.
Important Notes: During shipment, small volumes of PTPRC antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.
Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about PTPRC antibody, all related information, articles, resources about PTPRC antibody are being stored on our online database. Let us know if you have questions regarding this product.
Disclaimer: For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed. |
|
|
|
GenWay is a Protein and Antibody Solutions Provider. Customer Satisfaction is Our Top Priority.
|
|
Thank you for visiting GenWay Biotech's Datasheet. GenWay is the world's leader in chicken, IgY antibody production, producing over 900 quality polyclonal IgY antibodies to date. From our unique recombinant protein production, utilizing a variety of protein expression systems, GenWay's polyclonal IgY antibodies incorporate more epitopes, increasing the success rate for ELISA, western blot, immunohistochemistry (IHC), immunocytochemistry (ICC), and many more immunoassays. |
|
|
