ITGB3 antibody, CD61 Antibody, Hamster Monoclonal Antibody

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CD61 Antibody, Hamster Monoclonal Antibody

Catalog Number: 20-783-74068

Buy ITGB3 antibody -

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Related Product Names:

- ITGB3 antibody; ITGB3; GP3A; CD61
- HAMSTER ANTI MOUSE CD61: Low Endotoxin; Integrin beta-3

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: ITGB3	Gene Name Synonym: GP3A
Gi #: N/A	NCBI Acc #: NP_000203.2	Swiss Prot Acc #: O54890
Length (aa): N/A	Mol. Weight (Da): 87058	Chrom Location: N/A

Specificity: CD61

NCBI Gene ID: 16416

Isotype: Specificity: CD61

Clone: HM beta 3.1

Immunogen: Mouse alpha 5 beta 3 protein purified from the mouse hybridoma 2B4

Fusion Partner: Spleen cells from immunised Armenian hamsters were fused with cells of the P3U1 mouse myeloma cell line.

Specificity Note: This product recognises the murine integrin beta 3 subunit (CD61), a 90kD a type I membrane protein, which is expressed primarily on megakaryocytes, platelets, monocytes, macrophages, granulocytes and endothelial cells. CD61 associates with either the alpha IIb integrin (CD41) or the alpha V integrin (CD51) to form the platelet glycoprotein complex IIb/IIIa and the vitronectin receptor (VNR) respectively. The heterodimers formed with CD61 are receptors for a variety of ligands including fibrinogen, fibronectin, von Willebrands factor (vWF), vitronectin and thrombospondin.

Clone HM beta 3.1 is reported to partially inhibit the binding of CD61 to fibronectin (1).

Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution: Phosphate buffered saline pH7.4

Preservative Stabilisers: None present

Suggested Dilution: Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

Suggested Dilution: Flow Cytometry - 1/25 - 1/50

Source/Host: Hamster

Clonality: Monoclonal

Crossreactivity: Target Species: Mouse
Reacts with: Rat N.B.

Format: Low Endotoxin

Storage: Store at -20 degree C only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.

Stability: 18 months from date of despatch.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for ITGB3 ANTIBODY:

FACS/FC, FA, IP: Tested

TESTING: (secondary reagents and protocols )

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul. Method sheets are available on request ( anti-ITGB3 )

ITGB3 ANTIBODY TARGET DESCRIPTION:

Synonym Names for ITGB3 antibody: ITGB3; GP3A; HAMSTER ANTI MOUSE CD61: Low Endotoxin; Integrin beta-3

Function: Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

Subunit: Heterodimer of an alpha and a beta subunit. Beta-3 associates with either alpha-IIb or alpha-V. Isoform Beta-3C interacts with FLNB. Interacts with HIV-1 Tat.

Subcellular Location: Membrane; Single-pass type I membrane protein.

Tissue Specificity: Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.

Ptm: Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. SHC binding also occurs when Tyr-785 alone is phosphorylated.

Polymorphism: Position 59 is associated with platelet-specific alloantigen HPA-1 (ZW or PL(A)). HPA-1A/ZW(A)/PL(A1) has Leu-59 and HPA-1B/ZW(B)/PL(A2) has Pro-59.

Polymorphism: Position 169 is associated with platelet-specific alloantigen HPA-4 (PEN or YUK). HPA-4A/PEN(A)/YUK(A) has Arg-169 and HPA-4B/PEN(B)/YUK(B) has Gln-169. HPA-4B is involved in neonatal alloimmune thrombocytopenia (NAIT or NATP).

Polymorphism: Position 433 is associated with platelet-specific alloantigen MO. MO(-) has Pro-433 and MO(+) has Ala-433. MO(+) is involved in NAIT.

Polymorphism: Position 515 is associated with platelet-specific alloantigen CA/TU. CA(-)/TU(-) has Arg-515 and CA(+)/TU(+) has Gln-515. CA(+) is involved in NAIT.

Polymorphism: Position 662 is associated with platelet-specific alloantigen SR(A). SR(A)(-) has Arg-662 and SR(A)(+) has Cys-662.

Disease: Defects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. Its inheritance is autosomal recessive. It is characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb-IIIa complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the GPIIb-IIIa complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT variants have normal or near normal (60-100%) expression of dysfunctional receptors.

Similarity: Belongs to the integrin beta chain family.

Similarity: Contains 1 VWFA domain.

CD61 reacts with target species: mouse
reacts with: rat n.b..

OMIM: 173470; gene+phenotype. [NCBI / EBI]
273800; phenotype. [NCBI / EBI]

Pathways:
KEGG pathway: ECM-receptor interaction 04512
KEGG pathway: Focal adhesion 04510
KEGG pathway: Hematopoietic cell lineage 04640
KEGG pathway: Regulation of actin cytoskeleton 04810
Reactome Event:Hemostasis 109582
Reactome Event:Signaling in Immune system 168256

Products similar to ITGB3 antibody:

IgG

CD61, IgG

Integrin -beta3 (Phospho-Tyr785), IgG

Peptide

Integrin -beta3 (Phospho-Tyr773) Antibody Blocking Peptide, Peptide

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

BACKGROUND REFERENCES for ITGB3 ANTIBODY:

Background references for antibody target are not specific to GenWay products

1. Yasuda. M. et al. (1995) Expression and function of fibronectin binding integrins on rat mast cells.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: ITGB3 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of ITGB3 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about ITGB3 antibody, all related information, articles, resources about ITGB3 antibody are being stored on our online database. Let us know if you have questions regarding this product.

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