COL4A1 native protein, COLLAGEN IV native protein

Alphabetical Listing of Primary Antibodies:	f\| 0-4 \| 5-9 \| A \| B \| C \| D \| E \| F \| G \| H \| I \| J \| K \| L \| M \| N \| O \| P \| Q \| R \| S \| T \| U \| V \| W \| X \| Y \| Z \|
Alphabetical Listing of Proteins:	f\| 0-4 \| 5-9 \| A \| B \| C \| D \| E \| F \| G \| H \| I \| J \| K \| L \| M \| N \| O \| P \| Q \| R \| S \| T \| U \| V \| W \| X \| Y \| Z \|
Peptides \| Secondary Antibodies \| ELISA Kits

COLLAGEN IV native protein

Catalog Number: 11-783-79508

Buy COL4A1 native protein -

Size:

Related Product Names:

- COL4A1 native protein; COL4A1; COLLAGEN IV
- NATIVE HUMAN COLLAGEN IV; Collagen alpha-1(IV) chain

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: COL4A1	Gene Name Synonym: N/A
Gi #: N/A	NCBI Acc #: NP_001836.2	Swiss Prot Acc #: N/A
Length (aa): N/A	Mol. Weight (Da): 160615	Chrom Location: N/A

Specificity: COLLAGEN IV

Preparation: Salt and alcohol precipitations, DEAE chromatography. Purity and retention of native helical structure was controlled by SDS-PAGE, ORD measurement, ability to form microfibrils and by reaction with anti-collagen type specific monoclonal antibodies.

Buffer Solution: Phosphate buffered saline

Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)

Purity/Purification: SDS PAGE: >95%

Crossreactivity: Human

Format: Purified

Storage: Store at +4 degree C or at -20 degree C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

Stability: 18 months from date of despatch.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for COL4A1 NATIVE PROTEIN:

ELISA: Tested

TESTING: (secondary reagents and protocols )

Not Available

COL4A1 NATIVE PROTEIN TARGET DESCRIPTION:

Synonym Names for COL4A1 native protein: COL4A1; NATIVE HUMAN COLLAGEN IV; Collagen alpha-1(IV) chain

Function: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Potently inhibits endothelial cell proliferation and angiogenesis. Inhibits angiogenesis potentially via mechanisms involving cell surface proteoglycans and the alpha and beta integrins of endothelial cells.

Subunit: There are six type IV collagen isoforms, alpha 1(IV)-alpha 6(IV), each of which can form a triple helix structure with 2 other chains to generate type IV collagen network.

Subcellular Location: Secreted, extracellular space, extracellular matrix, basement membrane.

Tissue Specificity: Highly expressed in placenta.

Domain: Alpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain.

Ptm: Lysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in all cases and bind carbohydrates.

Ptm: Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

Ptm: Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.

Ptm: The trimeric structure of the NC1 domains may be stabilized by covalent bonds between Lys and Met residues.

Disease: Defects in COL4A1 are a cause of brain small vessel disease with hemorrhage [MIM:607595]. Brain small vessel diseases underlie 20 to 30 percent of ischemic strokes and a larger proportion of intracerebral hemorrhages. Inheritance is autosomal dominant.

Disease: Defects in COL4A1 are the cause of hereditary angiopathy with nephropathy, aneurysms, and muscle cramps (HANAC) [MIM:611773]. The clinical renal manifestations include hematuria and bilateral large cysts. Histologic analysis revealed complex basement membrane defects in kidney and skin. The systemic angiopathy appears to affect both small vessels and large arteries.

Disease: Defects in COL4A1 are a cause of porencephaly type 1 [MIM:175780]; also known as encephaloclastic porencephaly. Porencephaly is a term used for any cavitation or cerebrospinal fluid-filled cyst in the brain. Porencephaly type 1 is usually unilateral and results from focal destructive lesions such as fetal vascular occlusion or birth trauma. Inheritance is autosomal dominant.

Similarity: Belongs to the type IV collagen family.

Similarity: Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain.

COLLAGEN IV reacts with human.

OMIM: 120130; gene. [NCBI / EBI]
175780; phenotype. [NCBI / EBI]
607595; phenotype. [NCBI / EBI]
611773; phenotype. [NCBI / EBI]

Pathways:
KEGG pathway: Cell Communication 01430
KEGG pathway: ECM-receptor interaction 04512
KEGG pathway: Focal adhesion 04510
KEGG pathway: Small cell lung cancer 05222

Products similar to COL4A1 native protein:

IgG

Collagen IV, IgG

Collagen IV [COL-94], IgG

Collagen IV (Biotin), IgG

Collagen IV [SPM131] (ready-to-use), IgG

SUGGESTED PROTEIN REAGENTS - GenWay offers reagents to facilitate your research with cell-based assays, Mass Spectrometry, standards, calibrators, Western blot, cell culture, antibiotics, microwell substrates, and more!

BACKGROUND REFERENCES for COL4A1 NATIVE PROTEIN:

Background references for antibody target are not specific to GenWay products

1. Glanville. R.W. et al. (1979) Isolation and characterization of a native placental basement-membrane collagen and its component alpha chains 2. Sage. H. et al . (1979) Characterization of a novel collagen chain in human placenta and its relation to AB collagen 3. Klasson. S.C. et al . (1986) The effects of tissue pretreatment and pepsin levels on the isolation of collagens from human placenta. 4. Bornstein. P. (1980) Structurally distinct collagen types

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: COL4A1 native protein is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of COL4A1 native protein vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about COL4A1 native protein, all related information, articles, resources about COL4A1 native protein are being stored on our online database. Let us know if you have questions regarding this product.

Disclaimer: For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed.

GenWay is a Protein and Antibody Solutions Provider. Customer Satisfaction is Our Top Priority.

GenWay has a diverse line of recombinant protein expression platforms, including E. coli expression, Bacliovirus expression, yeast expression, and mammalian expression. Combining the technologies of bioinformatics, protein domain analysis, gene codon optimization, protein fusion, and protein expression in different host cells, GenWay can produce soluble and functional proteins. In particular, GenWay has the capability and vector systems for expression of difficult proteins such as proteases, kinases, glycosylated and membrane proteins. We have a very high success rate (almost 100%) and can deliver the proteins in a timely fashion. This is a core competency necessary for efficient and effective production of immunogens and affinity ligands for antibody production and purification. GenWay has over 300

recombinant proteins

offered in our catalog.



GenWay Biotech, Inc. 6777 Nancy Ridge Drive, San Diego, CA 92121 Tel: (858)458-0866 Fax: (858)458-0833 Email: sales@genwaybio.com www.genwaybio.com ©1998 - 2010 GenWay Biotech, Inc. - All Rights Reserved